The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them
Aim. To study the metabolomic profile of bacterial strains of the genus Bacillus promising for the development of biofungicides using thin layer chromatography (TLC), bioautography and high performance liquid chromatography with mass spectrometry (HPLC‐MS).Materials and Methods. The objects of study...
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Kamerton
2023-07-01
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| Series: | Юг России: экология, развитие |
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| Online Access: | https://ecodag.elpub.ru/ugro/article/view/2830 |
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| author | N. S. Tomashevich T. M. Sidorova V. V. Allahverdyan A. M. Asaturova |
| author_facet | N. S. Tomashevich T. M. Sidorova V. V. Allahverdyan A. M. Asaturova |
| author_sort | N. S. Tomashevich |
| collection | DOAJ |
| description | Aim. To study the metabolomic profile of bacterial strains of the genus Bacillus promising for the development of biofungicides using thin layer chromatography (TLC), bioautography and high performance liquid chromatography with mass spectrometry (HPLC‐MS).Materials and Methods. The objects of study are strains of bacteria of the genus Bacillus. Exometabolites were isolated from the liquid culture and their metabolomic profile was analyzed by TLC, bioautography, and HPLC‐MS.Results. By the method of bioautography with a test culture of the fungus F. oxysporum var. orthoceras BZR F6 metabolites of bacterial strains of the genus Bacillus surfactin, iturin and fengycin were identified. Quantitative analysis using HPLC‐MS analysis allows us to state that the B. velenzensis BZR 336g and B. amyloliquefaciens BZR 277 strains produce more surfactin than the others. An increased content of ituric lipopeptides was found in strains B. velezensis BZR 517 and B. velenzensis BZR 336g. According to the ability to produce fengycins, strains of B. velezensis BZR 517 and B. velenzensis BZR 336g are ahead of other strains.Conclusion. Studies using two analytical methods reveal that the strains produce all three antifungal lipopeptides. This is important, since metabolites are able not only to suppress phytopathogenic fungi, but also to enhance the antifungal effect due to synergism. The results obtained allow us to state the possibility of using all four strains as producers of effective biofungicides. |
| format | Article |
| id | doaj-art-1438e774687a4faf92d2e50f0a9808e9 |
| institution | DOAJ |
| issn | 1992-1098 2413-0958 |
| language | Russian |
| publishDate | 2023-07-01 |
| publisher | Kamerton |
| record_format | Article |
| series | Юг России: экология, развитие |
| spelling | doaj-art-1438e774687a4faf92d2e50f0a9808e92025-08-20T03:18:48ZrusKamertonЮг России: экология, развитие1992-10982413-09582023-07-0118210.18470/1992-1098-2023-2-70-811310The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on themN. S. Tomashevich0T. M. Sidorova1V. V. Allahverdyan2A. M. Asaturova3Federal Research Centre of Biological Plant ProtectionFederal Research Centre of Biological Plant ProtectionFederal Research Centre of Biological Plant ProtectionFederal Research Centre of Biological Plant ProtectionAim. To study the metabolomic profile of bacterial strains of the genus Bacillus promising for the development of biofungicides using thin layer chromatography (TLC), bioautography and high performance liquid chromatography with mass spectrometry (HPLC‐MS).Materials and Methods. The objects of study are strains of bacteria of the genus Bacillus. Exometabolites were isolated from the liquid culture and their metabolomic profile was analyzed by TLC, bioautography, and HPLC‐MS.Results. By the method of bioautography with a test culture of the fungus F. oxysporum var. orthoceras BZR F6 metabolites of bacterial strains of the genus Bacillus surfactin, iturin and fengycin were identified. Quantitative analysis using HPLC‐MS analysis allows us to state that the B. velenzensis BZR 336g and B. amyloliquefaciens BZR 277 strains produce more surfactin than the others. An increased content of ituric lipopeptides was found in strains B. velezensis BZR 517 and B. velenzensis BZR 336g. According to the ability to produce fengycins, strains of B. velezensis BZR 517 and B. velenzensis BZR 336g are ahead of other strains.Conclusion. Studies using two analytical methods reveal that the strains produce all three antifungal lipopeptides. This is important, since metabolites are able not only to suppress phytopathogenic fungi, but also to enhance the antifungal effect due to synergism. The results obtained allow us to state the possibility of using all four strains as producers of effective biofungicides.https://ecodag.elpub.ru/ugro/article/view/2830metabolites<i>bacillus velezensis</i>antifungal lipopeptidesgas chromatography‐mass spectrometrymetabolomic profile |
| spellingShingle | N. S. Tomashevich T. M. Sidorova V. V. Allahverdyan A. M. Asaturova The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them Юг России: экология, развитие metabolites <i>bacillus velezensis</i> antifungal lipopeptides gas chromatography‐mass spectrometry metabolomic profile |
| title | The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them |
| title_full | The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them |
| title_fullStr | The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them |
| title_full_unstemmed | The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them |
| title_short | The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them |
| title_sort | study of metabolites of new promising strains of bacterial antagonists of the genus i bacillus i to increase the effectiveness of fungicidal biological products based on them |
| topic | metabolites <i>bacillus velezensis</i> antifungal lipopeptides gas chromatography‐mass spectrometry metabolomic profile |
| url | https://ecodag.elpub.ru/ugro/article/view/2830 |
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