Screening assays for heart function mutants in Drosophila
The rapid life cycle and genetic tractability of Drosophila make it an ideal organism for large-scale genetic screens. Here we describe a novel assay for pupal heart rate and rhythmicity, as well as techniques to measure adult cardiac stress response. These assays can be powerfully combined to concu...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-07-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04371ST01 |
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| _version_ | 1850151683804364800 |
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| author | Robert J. Wessells Rolf Bodmer |
| author_facet | Robert J. Wessells Rolf Bodmer |
| author_sort | Robert J. Wessells |
| collection | DOAJ |
| description | The rapid life cycle and genetic tractability of Drosophila make it an ideal organism for large-scale genetic screens. Here we describe a novel assay for pupal heart rate and rhythmicity, as well as techniques to measure adult cardiac stress response. These assays can be powerfully combined to concurrently screen for both mutations affecting cardiac function and mutations affecting the age-dependent decline in adult cardiac stress response. Mutations identified in such screens have the potential to contribute greatly to the understanding of both congenital heart disease and the regulation of age-dependent decline in cardiac function in the human population. |
| format | Article |
| id | doaj-art-14300df035724dbc93bb82cf18f4eaf2 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2004-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-14300df035724dbc93bb82cf18f4eaf22025-08-20T02:26:09ZengTaylor & Francis GroupBioTechniques0736-62051940-98182004-07-01371586610.2144/04371ST01Screening assays for heart function mutants in DrosophilaRobert J. Wessells0Rolf Bodmer11The Burnham Institute, La Jolla, CA, USA1The Burnham Institute, La Jolla, CA, USAThe rapid life cycle and genetic tractability of Drosophila make it an ideal organism for large-scale genetic screens. Here we describe a novel assay for pupal heart rate and rhythmicity, as well as techniques to measure adult cardiac stress response. These assays can be powerfully combined to concurrently screen for both mutations affecting cardiac function and mutations affecting the age-dependent decline in adult cardiac stress response. Mutations identified in such screens have the potential to contribute greatly to the understanding of both congenital heart disease and the regulation of age-dependent decline in cardiac function in the human population.https://www.future-science.com/doi/10.2144/04371ST01 |
| spellingShingle | Robert J. Wessells Rolf Bodmer Screening assays for heart function mutants in Drosophila BioTechniques |
| title | Screening assays for heart function mutants in Drosophila |
| title_full | Screening assays for heart function mutants in Drosophila |
| title_fullStr | Screening assays for heart function mutants in Drosophila |
| title_full_unstemmed | Screening assays for heart function mutants in Drosophila |
| title_short | Screening assays for heart function mutants in Drosophila |
| title_sort | screening assays for heart function mutants in drosophila |
| url | https://www.future-science.com/doi/10.2144/04371ST01 |
| work_keys_str_mv | AT robertjwessells screeningassaysforheartfunctionmutantsindrosophila AT rolfbodmer screeningassaysforheartfunctionmutantsindrosophila |