In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)

Trees play an important role in urban landscapes by offering ecological and functional benefits such as heat mitigation and biodiversity support. Native fruit trees, such as the Rio Grande cherry (Eugenia involucrata DC.), may have great potential to be used in this context, by providing food for l...

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Main Authors: Marcelo Almeida de Oliveira Junior, Bruna do Amaral Brogio Colli, Liliane Cristina Libório Stipp, Rodrigo Rocha Latado, Sônia Maria de Stefano Piedade, Francisco de Assis Alves Mourao Filho
Format: Article
Language:English
Published: Sociedade Brasileira de Floricultura e Plantas Ornamentais 2025-06-01
Series:Ornamental Horticulture
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Online Access:https://ornamentalhorticulture.com.br/rbho/article/view/2852
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author Marcelo Almeida de Oliveira Junior
Bruna do Amaral Brogio Colli
Liliane Cristina Libório Stipp
Rodrigo Rocha Latado
Sônia Maria de Stefano Piedade
Francisco de Assis Alves Mourao Filho
author_facet Marcelo Almeida de Oliveira Junior
Bruna do Amaral Brogio Colli
Liliane Cristina Libório Stipp
Rodrigo Rocha Latado
Sônia Maria de Stefano Piedade
Francisco de Assis Alves Mourao Filho
author_sort Marcelo Almeida de Oliveira Junior
collection DOAJ
description Trees play an important role in urban landscapes by offering ecological and functional benefits such as heat mitigation and biodiversity support. Native fruit trees, such as the Rio Grande cherry (Eugenia involucrata DC.), may have great potential to be used in this context, by providing food for local fauna and promoting ecological balance, and easily adapt to this environment. On the other hand, propagation is limited by its annual fruiting cycle, recalcitrant seeds, and the low efficiency of clonal propagation methods. Tissue culture offers a promising alternative for large-scale seedling production using in vitro germinated seedlings for micropropagation. This study aimed to optimize in vitro establishment of Rio Grande cherry by evaluating seed preparation and cultivation conditions to enhance in vitro germination and nursery tree production. Factors such as light conditions, tegument removal, MS salt concentrations, sucrose concentrations, and seed fractioning were evaluated in several distinct experiments. High germination and seedling development rates were achieved by removing the tegument and cultivating seeds in the dark. Using a complete MS medium and supplementation of 15 g L -1 of sucrose also enhanced seedling development. Despite fractioned seeds having some germination potential, seedling development was negatively affected.
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publishDate 2025-06-01
publisher Sociedade Brasileira de Floricultura e Plantas Ornamentais
record_format Article
series Ornamental Horticulture
spelling doaj-art-142fcf48b2b44120a7132931a8d2aed52025-08-20T02:40:28ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2025-06-013110.1590/2447-536X.v31.e312852In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)Marcelo Almeida de Oliveira Junior0Bruna do Amaral Brogio Colli1Liliane Cristina Libório Stipp2Rodrigo Rocha Latado3Sônia Maria de Stefano Piedade4Francisco de Assis Alves Mourao Filho5Universidade de São PauloUniversidade de São PauloUniversidade de São PauloInstituto AgronômicoUniversidade de São PauloUniversidade de São Paulo Trees play an important role in urban landscapes by offering ecological and functional benefits such as heat mitigation and biodiversity support. Native fruit trees, such as the Rio Grande cherry (Eugenia involucrata DC.), may have great potential to be used in this context, by providing food for local fauna and promoting ecological balance, and easily adapt to this environment. On the other hand, propagation is limited by its annual fruiting cycle, recalcitrant seeds, and the low efficiency of clonal propagation methods. Tissue culture offers a promising alternative for large-scale seedling production using in vitro germinated seedlings for micropropagation. This study aimed to optimize in vitro establishment of Rio Grande cherry by evaluating seed preparation and cultivation conditions to enhance in vitro germination and nursery tree production. Factors such as light conditions, tegument removal, MS salt concentrations, sucrose concentrations, and seed fractioning were evaluated in several distinct experiments. High germination and seedling development rates were achieved by removing the tegument and cultivating seeds in the dark. Using a complete MS medium and supplementation of 15 g L -1 of sucrose also enhanced seedling development. Despite fractioned seeds having some germination potential, seedling development was negatively affected. https://ornamentalhorticulture.com.br/rbho/article/view/2852Atlantic ForestMyrtaceae native species tissue culture woody plant
spellingShingle Marcelo Almeida de Oliveira Junior
Bruna do Amaral Brogio Colli
Liliane Cristina Libório Stipp
Rodrigo Rocha Latado
Sônia Maria de Stefano Piedade
Francisco de Assis Alves Mourao Filho
In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)
Ornamental Horticulture
Atlantic Forest
Myrtaceae
native species
tissue culture
woody plant
title In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)
title_full In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)
title_fullStr In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)
title_full_unstemmed In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)
title_short In vitro seed germination and seedling development of Rio Grande cherry (Eugenia involucrata DC.)
title_sort in vitro seed germination and seedling development of rio grande cherry eugenia involucrata dc
topic Atlantic Forest
Myrtaceae
native species
tissue culture
woody plant
url https://ornamentalhorticulture.com.br/rbho/article/view/2852
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