Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM Technology
Single nucleotide polymorphism (SNP) association studies searching for differences in allele frequencies between cases and controls have been widely used for genetic analysis. Individual genotyping is prohibitively expensive in large sample sizes. Pooling of samples provides the obvious advantage of...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2002-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02325dd04 |
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| _version_ | 1850152195257794560 |
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| author | Jon Wasson Gary Skolnick Latisha Love-Gregory M. Alan Permutt |
| author_facet | Jon Wasson Gary Skolnick Latisha Love-Gregory M. Alan Permutt |
| author_sort | Jon Wasson |
| collection | DOAJ |
| description | Single nucleotide polymorphism (SNP) association studies searching for differences in allele frequencies between cases and controls have been widely used for genetic analysis. Individual genotyping is prohibitively expensive in large sample sizes. Pooling of samples provides the obvious advantage of higher throughput and lower cost. Here we report our results with the analysis of SNP allele frequencies in DNA pools using PyrosequencingTM technology. For seven different SNPs, we observed a mean difference of 1.1 ± 0.6% between allele frequencies determined in two different DNA pools (n = 150 cases and 150 controls) compared to individually genotyped samples. |
| format | Article |
| id | doaj-art-142aaeb6a55c42f4836661d6f48dec3b |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2002-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-142aaeb6a55c42f4836661d6f48dec3b2025-08-20T02:26:03ZengTaylor & Francis GroupBioTechniques0736-62051940-98182002-05-013251144115210.2144/02325dd04Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM TechnologyJon Wasson0Gary Skolnick1Latisha Love-Gregory2M. Alan Permutt31Washington University School of Medicine, St. Louis, MO, USA1Washington University School of Medicine, St. Louis, MO, USA1Washington University School of Medicine, St. Louis, MO, USA1Washington University School of Medicine, St. Louis, MO, USASingle nucleotide polymorphism (SNP) association studies searching for differences in allele frequencies between cases and controls have been widely used for genetic analysis. Individual genotyping is prohibitively expensive in large sample sizes. Pooling of samples provides the obvious advantage of higher throughput and lower cost. Here we report our results with the analysis of SNP allele frequencies in DNA pools using PyrosequencingTM technology. For seven different SNPs, we observed a mean difference of 1.1 ± 0.6% between allele frequencies determined in two different DNA pools (n = 150 cases and 150 controls) compared to individually genotyped samples.https://www.future-science.com/doi/10.2144/02325dd04 |
| spellingShingle | Jon Wasson Gary Skolnick Latisha Love-Gregory M. Alan Permutt Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM Technology BioTechniques |
| title | Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM Technology |
| title_full | Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM Technology |
| title_fullStr | Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM Technology |
| title_full_unstemmed | Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM Technology |
| title_short | Assessing Allele Frequencies of Single Nucleotide Polymorphisms in DNA Pools by PyrosequencingTM Technology |
| title_sort | assessing allele frequencies of single nucleotide polymorphisms in dna pools by pyrosequencingtm technology |
| url | https://www.future-science.com/doi/10.2144/02325dd04 |
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