Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i>
<i>Prunus avium</i> is a woody plant of economic importance within the genus <i>Prunus</i>, the family Rosaceae, which is affected by various environmental factors during its long growth period. Growth-regulating factors (<i>GRFs</i>) and GRF-interacting factors (...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/1/153 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589378565701632 |
|---|---|
| author | Hongxu Chen Yangang Pei Wanjia Tang Hongfen Li Yidi Huang Yizhe Chu Guanqiong Kou Wenyi Niu Runmei He Ronggao Gong |
| author_facet | Hongxu Chen Yangang Pei Wanjia Tang Hongfen Li Yidi Huang Yizhe Chu Guanqiong Kou Wenyi Niu Runmei He Ronggao Gong |
| author_sort | Hongxu Chen |
| collection | DOAJ |
| description | <i>Prunus avium</i> is a woody plant of economic importance within the genus <i>Prunus</i>, the family Rosaceae, which is affected by various environmental factors during its long growth period. Growth-regulating factors (<i>GRFs</i>) and GRF-interacting factors (<i>GIFs</i>) are essential in regulating plant growth and development, responding to environmental stresses, and responding to exogenous hormone induction. Genome-wide analysis showed 13 <i>GRF</i> genes on eight chromosomes and three <i>GIF</i> genes on three chromosomes in <i>P. avium</i>, clustered into three and two branches, respectively. Cis-acting element analysis indicated that the PavGRF promoters contained regulatory elements associated with hormones, light stress, and growth development. Therefore, we evaluated the effects of gibberellin and light stress on the GRF and GIF genes in <i>P. avium</i> at different stages. Transcriptome data revealed that five <i>PavGRFs</i> exhibited elevated expression levels during the green ripening and color conversion stages in <i>P. avium</i>, <i>PavGRF9</i> and <i>PavGIF1</i> displayed higher expression during the full red stage, and gibberellin treatment led to the upregulation of these five <i>PavGRFs</i> and <i>PavGIF1</i> during the full red stage. However, light stress did not significantly impact the expression of <i>PavGRFs</i> and <i>PavGIFs</i>. Additionally, miR396 could bind to the <i>PavGRFs</i>, thereby regulating the expression level of <i>PavGIF</i> after transcription. This study revealed the potential roles of the GRF and GIF transcription factor families in <i>P. avium</i> fruit growth and development, exogenous hormone treatment, and light stress, laying the foundation for further research on the roles of the <i>GRF</i> and <i>GIF</i> gene families in <i>P. avium</i>. |
| format | Article |
| id | doaj-art-1426395ab34b41689b0d087c26c36e76 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-1426395ab34b41689b0d087c26c36e762025-01-24T13:16:57ZengMDPI AGAgronomy2073-43952025-01-0115115310.3390/agronomy15010153Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i>Hongxu Chen0Yangang Pei1Wanjia Tang2Hongfen Li3Yidi Huang4Yizhe Chu5Guanqiong Kou6Wenyi Niu7Runmei He8Ronggao Gong9College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, China<i>Prunus avium</i> is a woody plant of economic importance within the genus <i>Prunus</i>, the family Rosaceae, which is affected by various environmental factors during its long growth period. Growth-regulating factors (<i>GRFs</i>) and GRF-interacting factors (<i>GIFs</i>) are essential in regulating plant growth and development, responding to environmental stresses, and responding to exogenous hormone induction. Genome-wide analysis showed 13 <i>GRF</i> genes on eight chromosomes and three <i>GIF</i> genes on three chromosomes in <i>P. avium</i>, clustered into three and two branches, respectively. Cis-acting element analysis indicated that the PavGRF promoters contained regulatory elements associated with hormones, light stress, and growth development. Therefore, we evaluated the effects of gibberellin and light stress on the GRF and GIF genes in <i>P. avium</i> at different stages. Transcriptome data revealed that five <i>PavGRFs</i> exhibited elevated expression levels during the green ripening and color conversion stages in <i>P. avium</i>, <i>PavGRF9</i> and <i>PavGIF1</i> displayed higher expression during the full red stage, and gibberellin treatment led to the upregulation of these five <i>PavGRFs</i> and <i>PavGIF1</i> during the full red stage. However, light stress did not significantly impact the expression of <i>PavGRFs</i> and <i>PavGIFs</i>. Additionally, miR396 could bind to the <i>PavGRFs</i>, thereby regulating the expression level of <i>PavGIF</i> after transcription. This study revealed the potential roles of the GRF and GIF transcription factor families in <i>P. avium</i> fruit growth and development, exogenous hormone treatment, and light stress, laying the foundation for further research on the roles of the <i>GRF</i> and <i>GIF</i> gene families in <i>P. avium</i>.https://www.mdpi.com/2073-4395/15/1/153<i>Prunus avium</i>growth regulatory factor (<i>GRF</i>)<i>GRF-interacting factor</i> (<i>GIF</i>)growth and developmentlight stressexogenous hormone |
| spellingShingle | Hongxu Chen Yangang Pei Wanjia Tang Hongfen Li Yidi Huang Yizhe Chu Guanqiong Kou Wenyi Niu Runmei He Ronggao Gong Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i> Agronomy <i>Prunus avium</i> growth regulatory factor (<i>GRF</i>) <i>GRF-interacting factor</i> (<i>GIF</i>) growth and development light stress exogenous hormone |
| title | Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i> |
| title_full | Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i> |
| title_fullStr | Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i> |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i> |
| title_short | Genome-Wide Identification and Expression Analysis of the <i>GRF</i> and <i>GIF</i> Gene Families in <i>Prunus avium</i> |
| title_sort | genome wide identification and expression analysis of the i grf i and i gif i gene families in i prunus avium i |
| topic | <i>Prunus avium</i> growth regulatory factor (<i>GRF</i>) <i>GRF-interacting factor</i> (<i>GIF</i>) growth and development light stress exogenous hormone |
| url | https://www.mdpi.com/2073-4395/15/1/153 |
| work_keys_str_mv | AT hongxuchen genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT yangangpei genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT wanjiatang genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT hongfenli genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT yidihuang genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT yizhechu genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT guanqiongkou genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT wenyiniu genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT runmeihe genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi AT ronggaogong genomewideidentificationandexpressionanalysisoftheigrfiandigifigenefamiliesiniprunusaviumi |