Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease
IntroductionHypoxia-inducible factors (HIF) regulate gene transcription, which aids hypoxia adaptation while promoting renal fibrosis. Non-transferrin-bound iron (NTBI) is a catalytic form of iron that can lead to oxidative damage. However, NTBI in cat biofluids has rarely been evaluated.AimsWe asse...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1482998/full |
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| author | Chien-Hui Chen Chien-Hui Chen Wei-Li Hsu Pei-Shiue Jason Tsai Chun-Fu Lai Meng-Ting Wu Ya-Jane Lee Ya-Jane Lee |
| author_facet | Chien-Hui Chen Chien-Hui Chen Wei-Li Hsu Pei-Shiue Jason Tsai Chun-Fu Lai Meng-Ting Wu Ya-Jane Lee Ya-Jane Lee |
| author_sort | Chien-Hui Chen |
| collection | DOAJ |
| description | IntroductionHypoxia-inducible factors (HIF) regulate gene transcription, which aids hypoxia adaptation while promoting renal fibrosis. Non-transferrin-bound iron (NTBI) is a catalytic form of iron that can lead to oxidative damage. However, NTBI in cat biofluids has rarely been evaluated.AimsWe assessed cat plasma and urine HIF-1α (pHIF-1α/uHIF-1α) concentrations and urine NTBI (uNTBI) concentrations to investigate their relationship with chronic kidney disease (CKD) severity.MethodspHIF-1α and uHIF-1α concentrations were measured using commercial ELISA kits, while uNTBI concentrations were detected by inductively coupled plasma mass spectrometry.ResultsHealthy cats (n = 35) and cats with CKD (n = 84) formed the study cohorts. pHIF-1α concentrations increased from 9.48 pg./mL (median) in the healthy cohort to 11.42 pg./mL in early-stage CKD cats but decreased to 8.50 pg./mL in late-stage CKD cats. uHIF-1α concentrations gradually decreased with a significant difference between the control group (44.61 pg./mL) and the late-stage CKD group (36.79 pg./mL, p < 0.001). Cats with proteinuria had significantly higher uNTBI concentrations (35.61 ppb) than non-proteinuric cats (25.13 ppb, p = 0.019). Finally, the concentrations of pHIF-1α and uHIF-1α were positively correlated independent of renal function.Conclusion and clinical importanceOverall, pHIF-1α and uHIF-1α concentrations are lower in advanced CKD cats, while uNTBI concentrations are significantly higher in proteinuric cats. |
| format | Article |
| id | doaj-art-142307aded44469c812ac4bb796cd3ee |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-142307aded44469c812ac4bb796cd3ee2025-08-20T02:34:44ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14829981482998Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney diseaseChien-Hui Chen0Chien-Hui Chen1Wei-Li Hsu2Pei-Shiue Jason Tsai3Chun-Fu Lai4Meng-Ting Wu5Ya-Jane Lee6Ya-Jane Lee7School of Veterinary Medicine, College of Bio-Resources and Agriculture, Institute of Veterinary Clinical Science, National Taiwan University, Taipei, TaiwanVeterinary Hospital, College of Bio-Resources and Agriculture, National Taiwan University, Taipei, TaiwanCollege of Veterinary Medicine, Graduate Institute of Microbiology and Public Health, National Chung-Hsing University, Taichung, TaiwanSchool of Veterinary Medicine, College of Bio-Resources and Agriculture, Institute of Veterinary Medicine, National Taiwan University, Taipei, TaiwanNational Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Chemistry, College of Science, National Taiwan University, Taipei, TaiwanSchool of Veterinary Medicine, College of Bio-Resources and Agriculture, Institute of Veterinary Clinical Science, National Taiwan University, Taipei, TaiwanVeterinary Hospital, College of Bio-Resources and Agriculture, National Taiwan University, Taipei, TaiwanIntroductionHypoxia-inducible factors (HIF) regulate gene transcription, which aids hypoxia adaptation while promoting renal fibrosis. Non-transferrin-bound iron (NTBI) is a catalytic form of iron that can lead to oxidative damage. However, NTBI in cat biofluids has rarely been evaluated.AimsWe assessed cat plasma and urine HIF-1α (pHIF-1α/uHIF-1α) concentrations and urine NTBI (uNTBI) concentrations to investigate their relationship with chronic kidney disease (CKD) severity.MethodspHIF-1α and uHIF-1α concentrations were measured using commercial ELISA kits, while uNTBI concentrations were detected by inductively coupled plasma mass spectrometry.ResultsHealthy cats (n = 35) and cats with CKD (n = 84) formed the study cohorts. pHIF-1α concentrations increased from 9.48 pg./mL (median) in the healthy cohort to 11.42 pg./mL in early-stage CKD cats but decreased to 8.50 pg./mL in late-stage CKD cats. uHIF-1α concentrations gradually decreased with a significant difference between the control group (44.61 pg./mL) and the late-stage CKD group (36.79 pg./mL, p < 0.001). Cats with proteinuria had significantly higher uNTBI concentrations (35.61 ppb) than non-proteinuric cats (25.13 ppb, p = 0.019). Finally, the concentrations of pHIF-1α and uHIF-1α were positively correlated independent of renal function.Conclusion and clinical importanceOverall, pHIF-1α and uHIF-1α concentrations are lower in advanced CKD cats, while uNTBI concentrations are significantly higher in proteinuric cats.https://www.frontiersin.org/articles/10.3389/fvets.2024.1482998/fullHIFNTBICKDnoninvasive biomarkersrenal disease |
| spellingShingle | Chien-Hui Chen Chien-Hui Chen Wei-Li Hsu Pei-Shiue Jason Tsai Chun-Fu Lai Meng-Ting Wu Ya-Jane Lee Ya-Jane Lee Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease Frontiers in Veterinary Science HIF NTBI CKD noninvasive biomarkers renal disease |
| title | Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease |
| title_full | Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease |
| title_fullStr | Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease |
| title_full_unstemmed | Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease |
| title_short | Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease |
| title_sort | evaluation of hypoxia inducible factor 1α and urine non transferrin bound iron concentrations in cats with chronic kidney disease |
| topic | HIF NTBI CKD noninvasive biomarkers renal disease |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1482998/full |
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