Biosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospects
Background/Aim: Inonotus obliquus, commonly known as the Chaga mushroom, is an extensively studied source of bioactive compounds, including polyphenols, triterpenoids and polysaccharides. These metabolites exhibit potent antioxidant, anti-inflammatory and immunomodulatory properties, underscoring I...
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| Format: | Article |
| Language: | English |
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Medical Society of the Republic of Srpska, Banja Luka, University of Banja Luka. Faculty of Medicine
2025-01-01
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| Series: | Scripta Medica |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/2490-3329/2025/2490-33292502221P.pdf |
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| author | Parshenkov Mikhail Alekseevich Skovorodko Polina Petrovna Petrusevich Danila Alexandrovich Makaeva Shagun Narimanovna Osipova Svetlana Guramovna Ibragimova Gumru Istyagina Alexandra Olegovna Balaeva Kristina Anatolievna |
| author_facet | Parshenkov Mikhail Alekseevich Skovorodko Polina Petrovna Petrusevich Danila Alexandrovich Makaeva Shagun Narimanovna Osipova Svetlana Guramovna Ibragimova Gumru Istyagina Alexandra Olegovna Balaeva Kristina Anatolievna |
| author_sort | Parshenkov Mikhail Alekseevich |
| collection | DOAJ |
| description | Background/Aim: Inonotus obliquus, commonly known as the Chaga mushroom, is an extensively studied source of bioactive compounds, including polyphenols, triterpenoids and polysaccharides. These metabolites exhibit potent antioxidant, anti-inflammatory and immunomodulatory properties, underscoring I obliquus as a valuable resource for advanced biopharmaceuticals and functional therapeutics. Yet, achieving consistent and high-yield biosynthesis of these compounds remains challenging. Aim of this study was to develop a biotechnological approach leveraging co-cultivation with the bacterial cellulose producer Gluconacetobacter hansenii to improve both the yield and stability of these bioactive metabolites. Methods: I obliquus was cultivated on solid and liquid-phase substrates, with and without G hansenii co-cultivation. Biomass yield, morphological changes and growth dynamics were assessed. Total phenolic content and antioxidant activity were quantified using the Folin-Ciocalteu method and DPPH/ABTS assays. Anti-inflammatory properties were evaluated by measuring IL-6, TNF-a and IL-1b levels in LPS-stimulated THP-1 macrophages via ELISA. Results: Co-cultivation with G hansenii resulted in a 1.8-fold increase in fungal biomass yield (30.6 g/L on HS medium) and a 1.3-fold enhancement in phenolic content (85.4 mg GAE/g) compared to monoculture. Extracts from HS medium exhibited the highest antioxidant activity (IC50 = 43.2 µg/mL in DPPH and 29.8 µg/mL in ABTS assays) and significantly reduced pro-inflammatory cytokine levels (IL-6 by 47.5 %, TNF-a by 42.8 % and IL-1b by 39.6 %) in LPS-stimulated macrophages. Morphological analysis revealed a denser, more organised fungal network under co-cultivation, suggesting improved metabolic efficiency and bioactive compound synthesis. Conclusion: Co-cultivation with G hansenii provides a scalable and efficient strategy to enhance the bioactive potential of I obliquus. This approach significantly improves metabolite yield, antioxidant capacity and anti-inflammatory activity, opening new avenues for the development of standardised therapeutic formulations targeting oxidative stress and immune modulation. |
| format | Article |
| id | doaj-art-141e5673943a4d369906cf448a68302a |
| institution | DOAJ |
| issn | 2490-3329 2303-7954 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Medical Society of the Republic of Srpska, Banja Luka, University of Banja Luka. Faculty of Medicine |
| record_format | Article |
| series | Scripta Medica |
| spelling | doaj-art-141e5673943a4d369906cf448a68302a2025-08-20T03:19:25ZengMedical Society of the Republic of Srpska, Banja Luka, University of Banja Luka. Faculty of MedicineScripta Medica2490-33292303-79542025-01-0156222123110.5937/scriptamed56-574362490-33292502221PBiosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospectsParshenkov Mikhail Alekseevich0https://orcid.org/0009-0004-7170-8783Skovorodko Polina Petrovna1https://orcid.org/0009-0000-5624-4731Petrusevich Danila Alexandrovich2https://orcid.org/0009-0005-2283-6372Makaeva Shagun Narimanovna3https://orcid.org/0009-0008-3396-6684Osipova Svetlana Guramovna4https://orcid.org/0009-0006-6457-9543Ibragimova Gumru5https://orcid.org/0009-0007-0478-7137Istyagina Alexandra Olegovna6https://orcid.org/0009-0001-3295-8462Balaeva Kristina Anatolievna7https://orcid.org/0009-0002-2930-6006I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaI.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaI.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaI.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaI.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaI.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaI.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaI.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaBackground/Aim: Inonotus obliquus, commonly known as the Chaga mushroom, is an extensively studied source of bioactive compounds, including polyphenols, triterpenoids and polysaccharides. These metabolites exhibit potent antioxidant, anti-inflammatory and immunomodulatory properties, underscoring I obliquus as a valuable resource for advanced biopharmaceuticals and functional therapeutics. Yet, achieving consistent and high-yield biosynthesis of these compounds remains challenging. Aim of this study was to develop a biotechnological approach leveraging co-cultivation with the bacterial cellulose producer Gluconacetobacter hansenii to improve both the yield and stability of these bioactive metabolites. Methods: I obliquus was cultivated on solid and liquid-phase substrates, with and without G hansenii co-cultivation. Biomass yield, morphological changes and growth dynamics were assessed. Total phenolic content and antioxidant activity were quantified using the Folin-Ciocalteu method and DPPH/ABTS assays. Anti-inflammatory properties were evaluated by measuring IL-6, TNF-a and IL-1b levels in LPS-stimulated THP-1 macrophages via ELISA. Results: Co-cultivation with G hansenii resulted in a 1.8-fold increase in fungal biomass yield (30.6 g/L on HS medium) and a 1.3-fold enhancement in phenolic content (85.4 mg GAE/g) compared to monoculture. Extracts from HS medium exhibited the highest antioxidant activity (IC50 = 43.2 µg/mL in DPPH and 29.8 µg/mL in ABTS assays) and significantly reduced pro-inflammatory cytokine levels (IL-6 by 47.5 %, TNF-a by 42.8 % and IL-1b by 39.6 %) in LPS-stimulated macrophages. Morphological analysis revealed a denser, more organised fungal network under co-cultivation, suggesting improved metabolic efficiency and bioactive compound synthesis. Conclusion: Co-cultivation with G hansenii provides a scalable and efficient strategy to enhance the bioactive potential of I obliquus. This approach significantly improves metabolite yield, antioxidant capacity and anti-inflammatory activity, opening new avenues for the development of standardised therapeutic formulations targeting oxidative stress and immune modulation.https://scindeks-clanci.ceon.rs/data/pdf/2490-3329/2025/2490-33292502221P.pdfinonotus obliquuscellulose, bacterialantioxidantsactivityanti-inflammatory effectsbiotechnological cultivationphytochemicals |
| spellingShingle | Parshenkov Mikhail Alekseevich Skovorodko Polina Petrovna Petrusevich Danila Alexandrovich Makaeva Shagun Narimanovna Osipova Svetlana Guramovna Ibragimova Gumru Istyagina Alexandra Olegovna Balaeva Kristina Anatolievna Biosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospects Scripta Medica inonotus obliquus cellulose, bacterial antioxidants activity anti-inflammatory effects biotechnological cultivation phytochemicals |
| title | Biosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospects |
| title_full | Biosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospects |
| title_fullStr | Biosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospects |
| title_full_unstemmed | Biosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospects |
| title_short | Biosynthetic potential of Inonotus obliquus in symbiosis with bacterial cellulose: Optimisation and prospects |
| title_sort | biosynthetic potential of inonotus obliquus in symbiosis with bacterial cellulose optimisation and prospects |
| topic | inonotus obliquus cellulose, bacterial antioxidants activity anti-inflammatory effects biotechnological cultivation phytochemicals |
| url | https://scindeks-clanci.ceon.rs/data/pdf/2490-3329/2025/2490-33292502221P.pdf |
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