Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell Model
The vascular system is primarily responsible for orchestrating the underlying healing processes to achieve tissue regeneration, thus the promotion of angiogenic events could be a useful strategy to repair injured tissues. Among several approaches to stimulate tissue regeneration, non-invasive device...
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2025-02-01
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| author | Alessia Ricci Amelia Cataldi Marialucia Gallorini Viviana di Giacomo Monica Rapino Natalia Di Pietro Marco Mantarro Adriano Piattelli Susi Zara |
| author_facet | Alessia Ricci Amelia Cataldi Marialucia Gallorini Viviana di Giacomo Monica Rapino Natalia Di Pietro Marco Mantarro Adriano Piattelli Susi Zara |
| author_sort | Alessia Ricci |
| collection | DOAJ |
| description | The vascular system is primarily responsible for orchestrating the underlying healing processes to achieve tissue regeneration, thus the promotion of angiogenic events could be a useful strategy to repair injured tissues. Among several approaches to stimulate tissue regeneration, non-invasive devices are currently widely diffused. Complex Magnetic Fields (CMFs) are innovative pulsed multifrequency electromagnetic fields used for their promising results in clinical applications, such as diabetic foot treatment or edema resorption. Nevertheless, few papers are available demonstrating the biological mechanisms involved. In this paper, in order to understand CMFs’ capability to promote angiogenic events, Regenerative Tissue Program (RTP) was applied to an in vitro Endothelial Cells (ECs) model. ECs were stimulated with (I) 2 RTP consecutive cycles, (II) with an interval of 8 h (T0 + T8), or (III) 24 h (T0 + T24) from one cycle to another. Results demonstrate that (I) extracellular matrix degradation is promoted through matrix metalloproteinases 2 and 9 modulation, leading to an increased cell migratory capability; (II) CMFs support EC growth, activating Integrin β1-Erk-Cdk2 pathway and sustaining G1/S transition; (III) vessel morphogenesis is promoted when CMFs are applied. In conclusion, the promising clinical results are supported by in vitro analyses which evidence that main angiogenic events are stimulated by CMFs. |
| format | Article |
| id | doaj-art-141ceaecf7f445b59f7c12c901df6191 |
| institution | DOAJ |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-141ceaecf7f445b59f7c12c901df61912025-08-20T02:58:58ZengMDPI AGCells2073-44092025-02-0114533210.3390/cells14050332Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell ModelAlessia Ricci0Amelia Cataldi1Marialucia Gallorini2Viviana di Giacomo3Monica Rapino4Natalia Di Pietro5Marco Mantarro6Adriano Piattelli7Susi Zara8Department of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyUnit of Chieti, Genetic Molecular Institute of CNR, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyIndependent Researcher, MFI Srl, 00163 Rome, ItalySchool of Dentistry, UniCamillus-Saint Camillus International University of Health and Medical Sciences, 00131 Rome, ItalyDepartment of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyThe vascular system is primarily responsible for orchestrating the underlying healing processes to achieve tissue regeneration, thus the promotion of angiogenic events could be a useful strategy to repair injured tissues. Among several approaches to stimulate tissue regeneration, non-invasive devices are currently widely diffused. Complex Magnetic Fields (CMFs) are innovative pulsed multifrequency electromagnetic fields used for their promising results in clinical applications, such as diabetic foot treatment or edema resorption. Nevertheless, few papers are available demonstrating the biological mechanisms involved. In this paper, in order to understand CMFs’ capability to promote angiogenic events, Regenerative Tissue Program (RTP) was applied to an in vitro Endothelial Cells (ECs) model. ECs were stimulated with (I) 2 RTP consecutive cycles, (II) with an interval of 8 h (T0 + T8), or (III) 24 h (T0 + T24) from one cycle to another. Results demonstrate that (I) extracellular matrix degradation is promoted through matrix metalloproteinases 2 and 9 modulation, leading to an increased cell migratory capability; (II) CMFs support EC growth, activating Integrin β1-Erk-Cdk2 pathway and sustaining G1/S transition; (III) vessel morphogenesis is promoted when CMFs are applied. In conclusion, the promising clinical results are supported by in vitro analyses which evidence that main angiogenic events are stimulated by CMFs.https://www.mdpi.com/2073-4409/14/5/332endothelial cellsangiogenesiscomplex magnetic fieldsextracellular matrix remodelingtissue regenerationhealing processes |
| spellingShingle | Alessia Ricci Amelia Cataldi Marialucia Gallorini Viviana di Giacomo Monica Rapino Natalia Di Pietro Marco Mantarro Adriano Piattelli Susi Zara Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell Model Cells endothelial cells angiogenesis complex magnetic fields extracellular matrix remodeling tissue regeneration healing processes |
| title | Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell Model |
| title_full | Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell Model |
| title_fullStr | Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell Model |
| title_full_unstemmed | Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell Model |
| title_short | Angiogenic Events Positively Modulated by Complex Magnetic Fields in an In Vitro Endothelial Cell Model |
| title_sort | angiogenic events positively modulated by complex magnetic fields in an in vitro endothelial cell model |
| topic | endothelial cells angiogenesis complex magnetic fields extracellular matrix remodeling tissue regeneration healing processes |
| url | https://www.mdpi.com/2073-4409/14/5/332 |
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