Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo Study
<b>Background/Objectives:</b><i>Porphyromonas gingivalis</i> infection has been associated with various systemic diseases and may cause delayed healing of muscle injury. However, the relationship between muscle injury healing and <i>P. gingivalis</i> infection rem...
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2024-10-01
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| author | Shintaro Shimizu Kairi Hayashi Yasuo Takeuchi Gen Tanabe Hiroshi Churei Hiroaki Kobayashi Toshiaki Ueno Kenji Fueki |
| author_facet | Shintaro Shimizu Kairi Hayashi Yasuo Takeuchi Gen Tanabe Hiroshi Churei Hiroaki Kobayashi Toshiaki Ueno Kenji Fueki |
| author_sort | Shintaro Shimizu |
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| description | <b>Background/Objectives:</b><i>Porphyromonas gingivalis</i> infection has been associated with various systemic diseases and may cause delayed healing of muscle injury. However, the relationship between muscle injury healing and <i>P. gingivalis</i> infection remains unclear. Our hypothesis was that <i>P. gingivalis</i> infection delays the healing of muscle injuries. <b>Methods:</b> Fifty-six 8-week-old male Wistar rats were randomly divided into two groups: sonicated <i>P. gingivalis</i> was intraperitoneally administered in one group (PG group), whereas saline was administered in the other group (CO group). Skeletal muscle injury was induced via cardiotoxin injections in all animals. The cross-sectional area of regenerating muscle cells was evaluated by haematoxylin–eosin staining, and the degree of muscle fibrosis was evaluated by Masson’s trichrome staining. The expression of paired box protein (Pax7) and myoblast determination protein (MyoD) and the identified stages of myocyte regeneration were analysed by immunohistochemical staining. Motion analysis was performed during walking. <b>Results:</b> The cross-sectional area of muscle cells was significantly smaller in the PG group on days 7 and 14 post-injury than in the CO group. The Pax7+/MyoD− ratio was significantly lower in the PG group on day 1 post-injury than in the CO group. Motion analysis of treadmill walking showed that the PG group had a lower minimum calcaneal height on days 3 and 7 post-injury than the CO group. <b>Conclusions:</b> This study suggests that administration of sonicated <i>P. gingivalis</i> in rats can delay the healing process of muscle injury. Further research is needed to understand this mechanism of delay of <i>P. gingivalis</i>. |
| format | Article |
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| language | English |
| publishDate | 2024-10-01 |
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| series | Dentistry Journal |
| spelling | doaj-art-140e4e8ac232431ca3edbee6c4d9993a2025-08-20T01:53:44ZengMDPI AGDentistry Journal2304-67672024-10-01121134610.3390/dj12110346Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo StudyShintaro Shimizu0Kairi Hayashi1Yasuo Takeuchi2Gen Tanabe3Hiroshi Churei4Hiroaki Kobayashi5Toshiaki Ueno6Kenji Fueki7Department of Masticatory Function and Health Science, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8549, JapanDepartment of Masticatory Function and Health Science, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8549, JapanDepartment of Lifetime Oral Health Care Science, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8549, JapanDepartment of Sports Dentistry, School of Dentistry, Meikai University, Saitama 350-0283, JapanDepartment of Masticatory Function and Health Science, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8549, JapanDepartment of Periodontology, Graduate School of Medical and Dental Science, Institute of Science Tokyo, Tokyo 113-8549, JapanDepartment of Sports Dentistry, School of Dentistry, Meikai University, Saitama 350-0283, JapanDepartment of Masticatory Function and Health Science, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8549, Japan<b>Background/Objectives:</b><i>Porphyromonas gingivalis</i> infection has been associated with various systemic diseases and may cause delayed healing of muscle injury. However, the relationship between muscle injury healing and <i>P. gingivalis</i> infection remains unclear. Our hypothesis was that <i>P. gingivalis</i> infection delays the healing of muscle injuries. <b>Methods:</b> Fifty-six 8-week-old male Wistar rats were randomly divided into two groups: sonicated <i>P. gingivalis</i> was intraperitoneally administered in one group (PG group), whereas saline was administered in the other group (CO group). Skeletal muscle injury was induced via cardiotoxin injections in all animals. The cross-sectional area of regenerating muscle cells was evaluated by haematoxylin–eosin staining, and the degree of muscle fibrosis was evaluated by Masson’s trichrome staining. The expression of paired box protein (Pax7) and myoblast determination protein (MyoD) and the identified stages of myocyte regeneration were analysed by immunohistochemical staining. Motion analysis was performed during walking. <b>Results:</b> The cross-sectional area of muscle cells was significantly smaller in the PG group on days 7 and 14 post-injury than in the CO group. The Pax7+/MyoD− ratio was significantly lower in the PG group on day 1 post-injury than in the CO group. Motion analysis of treadmill walking showed that the PG group had a lower minimum calcaneal height on days 3 and 7 post-injury than the CO group. <b>Conclusions:</b> This study suggests that administration of sonicated <i>P. gingivalis</i> in rats can delay the healing process of muscle injury. Further research is needed to understand this mechanism of delay of <i>P. gingivalis</i>.https://www.mdpi.com/2304-6767/12/11/346<i>P. gingivalis</i>periodontitismotion analysisskeletal muscle injurysatellite cell |
| spellingShingle | Shintaro Shimizu Kairi Hayashi Yasuo Takeuchi Gen Tanabe Hiroshi Churei Hiroaki Kobayashi Toshiaki Ueno Kenji Fueki Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo Study Dentistry Journal <i>P. gingivalis</i> periodontitis motion analysis skeletal muscle injury satellite cell |
| title | Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo Study |
| title_full | Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo Study |
| title_fullStr | Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo Study |
| title_full_unstemmed | Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo Study |
| title_short | Effect of <i>Porphyromonas gingivalis</i> Infection on Healing of Skeletal Muscle Injury: An In Vivo Study |
| title_sort | effect of i porphyromonas gingivalis i infection on healing of skeletal muscle injury an in vivo study |
| topic | <i>P. gingivalis</i> periodontitis motion analysis skeletal muscle injury satellite cell |
| url | https://www.mdpi.com/2304-6767/12/11/346 |
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