Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit model
Abstract Background To investigate Quorum-Sensing inhibition by furanone compounds in Pseudomonas aeruginosa keratitis rabbit model. Methods Thirty adult New Zealand White rabbits were used. Anesthetized rabbits were intrastromally injected with Pseudomonas aeruginosa (P. aeruginosa). The rabbits we...
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BMC
2025-04-01
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| Series: | BMC Ophthalmology |
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| Online Access: | https://doi.org/10.1186/s12886-025-04061-4 |
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| author | Aleyna Dal Rümeysa Hekimoğlu Bilge Sümbül Fatmanur Babalı Balıbey Zeynep Özman Gökçe Ersin Akbulut Ahmet Elbay |
| author_facet | Aleyna Dal Rümeysa Hekimoğlu Bilge Sümbül Fatmanur Babalı Balıbey Zeynep Özman Gökçe Ersin Akbulut Ahmet Elbay |
| author_sort | Aleyna Dal |
| collection | DOAJ |
| description | Abstract Background To investigate Quorum-Sensing inhibition by furanone compounds in Pseudomonas aeruginosa keratitis rabbit model. Methods Thirty adult New Zealand White rabbits were used. Anesthetized rabbits were intrastromally injected with Pseudomonas aeruginosa (P. aeruginosa). The rabbits were divided into six groups: the control group (infected only with P. aeruginosa), group A (50 mg/mL ceftazidime), group B (0.1 mg/mL furanone), group C (0.2 mg/mL furanone), group D (0.3 mg/mL furanone), and group E (20% dimethyl sulfoxide). One drop of the treatment was applied every hour for 3 days, starting 1-h post-inoculation. Rabbits were then sacrificed, and corneas were analysed clinically, microbiologically, histologically, and biochemically. One-way analysis of variance was used for the mean comparison of independent groups. The Least Significant Difference method was used as a post-hoc test for pairwise comparisons. Results In all evaluations, the antibiotic group (group A) showed the best therapeutic response. The slit-lamp examination score of group C was significantly lowered than those compared of to the control (p = 0.009) and E groups (p = 0.014). Histological evaluation showed that inflammation is decreased in groups B, C, and D. Levels of cyclooxygenase-2 (COX-2), superoxide dismutase-1, and reactive oxygen species (ROS) were lowest in the antibiotic-treated group, whereas the highest levels were detected in the control group. Notably, the COX-2 levels in group B and ROS levels in groups B and C were significantly lower than in control group. (p = 0.045, p = 0.039 and p = 0.045, respectively). Conclusion Furanone compounds may have minimal therapeutic effects against Pseudomonas keratitis. Its therapeutic effect has not been observed to be sufficient compared with that of antibiotics. Further studies are needed to investigate their protective effects and mechanisms. |
| format | Article |
| id | doaj-art-13ffcd66f0cb41f2a7b3a9dbe059651d |
| institution | DOAJ |
| issn | 1471-2415 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| series | BMC Ophthalmology |
| spelling | doaj-art-13ffcd66f0cb41f2a7b3a9dbe059651d2025-08-20T03:14:07ZengBMCBMC Ophthalmology1471-24152025-04-0125111010.1186/s12886-025-04061-4Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit modelAleyna Dal0Rümeysa Hekimoğlu1Bilge Sümbül2Fatmanur Babalı Balıbey3Zeynep Özman Gökçe4Ersin Akbulut5Ahmet Elbay6Faculty of Medicine, Bezmialem Vakıf UniversityFaculty of Medicine, Department of Histology and Embryology, Bezmialem Vakıf UniversityFaculty of Medicine, Department of Clinical Microbiology, Bezmialem Vakıf UniversityFaculty of Medicine, Department of Medical Biochemistry, Bezmialem Vakıf UniversityDepartment of Medical Biochemistry, Institute of Health Sciences, Bezmialem Vakıf UniversityFaculty of Medicine, Department of Ophthalmology, Bezmialem Vakıf UniversityFaculty of Medicine, Department of Ophthalmology, Bezmialem Vakıf UniversityAbstract Background To investigate Quorum-Sensing inhibition by furanone compounds in Pseudomonas aeruginosa keratitis rabbit model. Methods Thirty adult New Zealand White rabbits were used. Anesthetized rabbits were intrastromally injected with Pseudomonas aeruginosa (P. aeruginosa). The rabbits were divided into six groups: the control group (infected only with P. aeruginosa), group A (50 mg/mL ceftazidime), group B (0.1 mg/mL furanone), group C (0.2 mg/mL furanone), group D (0.3 mg/mL furanone), and group E (20% dimethyl sulfoxide). One drop of the treatment was applied every hour for 3 days, starting 1-h post-inoculation. Rabbits were then sacrificed, and corneas were analysed clinically, microbiologically, histologically, and biochemically. One-way analysis of variance was used for the mean comparison of independent groups. The Least Significant Difference method was used as a post-hoc test for pairwise comparisons. Results In all evaluations, the antibiotic group (group A) showed the best therapeutic response. The slit-lamp examination score of group C was significantly lowered than those compared of to the control (p = 0.009) and E groups (p = 0.014). Histological evaluation showed that inflammation is decreased in groups B, C, and D. Levels of cyclooxygenase-2 (COX-2), superoxide dismutase-1, and reactive oxygen species (ROS) were lowest in the antibiotic-treated group, whereas the highest levels were detected in the control group. Notably, the COX-2 levels in group B and ROS levels in groups B and C were significantly lower than in control group. (p = 0.045, p = 0.039 and p = 0.045, respectively). Conclusion Furanone compounds may have minimal therapeutic effects against Pseudomonas keratitis. Its therapeutic effect has not been observed to be sufficient compared with that of antibiotics. Further studies are needed to investigate their protective effects and mechanisms.https://doi.org/10.1186/s12886-025-04061-4FuranoneKeratitisPseudomonas aeruginosaQuorum-Sensing |
| spellingShingle | Aleyna Dal Rümeysa Hekimoğlu Bilge Sümbül Fatmanur Babalı Balıbey Zeynep Özman Gökçe Ersin Akbulut Ahmet Elbay Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit model BMC Ophthalmology Furanone Keratitis Pseudomonas aeruginosa Quorum-Sensing |
| title | Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit model |
| title_full | Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit model |
| title_fullStr | Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit model |
| title_full_unstemmed | Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit model |
| title_short | Quorum-Sensing inhibition by furanone compounds and therapeutic effects on Pseudomonas aeruginosa keratitis rabbit model |
| title_sort | quorum sensing inhibition by furanone compounds and therapeutic effects on pseudomonas aeruginosa keratitis rabbit model |
| topic | Furanone Keratitis Pseudomonas aeruginosa Quorum-Sensing |
| url | https://doi.org/10.1186/s12886-025-04061-4 |
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