Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung Tumors
Introduction: Natural killer (NK) cells are innate lymphoid cells capable of directly killing target cells while modulating immune effector responses. Despite their multifunctional capacities, a limited understanding of their plasticity and heterogeneity has impeded progress in developing...
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| Format: | Article |
| Language: | English |
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Karger Publishers
2024-01-01
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| Series: | Journal of Innate Immunity |
| Online Access: | https://karger.com/article/doi/10.1159/000542078 |
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| author | Zerick Terrell Dunbar Salvador González-Ochoa Thanigaivelan Kanagasabai Alla Ivanova Anil Shanker |
| author_facet | Zerick Terrell Dunbar Salvador González-Ochoa Thanigaivelan Kanagasabai Alla Ivanova Anil Shanker |
| author_sort | Zerick Terrell Dunbar |
| collection | DOAJ |
| description |
Introduction: Natural killer (NK) cells are innate lymphoid cells capable of directly killing target cells while modulating immune effector responses. Despite their multifunctional capacities, a limited understanding of their plasticity and heterogeneity has impeded progress in developing effective NK cell-based cancer therapies. In this study, we investigated NK cell tissue heterogeneity in relation to their phenotype and effector functions against lung tumors. Methods: Using hanging drop tumor spheroid and subcutaneously established LL/2 (LLC1) lung tumor models, we examined NK cell receptor diversity and its correlation with tissue-specific cytotoxicity through multiparametric flow cytometry, fluorescence imaging, and cytotoxicity assays. Results: We identified distinct patterns of cell surface receptors expression on tissue-specific NK cells that are crucial for antitumor activity. Linear regression mathematical analyses further revealed significant positive correlations between activation-associated cell surface receptors and cytotoxic capacity in NK cells from tissues such as the liver and bone marrow. Conclusion: These findings underscore the differential effector capacities of NK cells from distinct tissues, even prior to exposure to LL/2 tumor cells. This highlights the significance of tissue-specific NK cell heterogeneity and its impact on their antitumor cytotoxicity. Recognizing these distinct tissue-specific receptor expression patterns will be instrumental in developing more efficacious NK cell-based cancer treatments. |
| format | Article |
| id | doaj-art-13ee3a276ef349f683dbb7e46acbd601 |
| institution | DOAJ |
| issn | 1662-8128 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Journal of Innate Immunity |
| spelling | doaj-art-13ee3a276ef349f683dbb7e46acbd6012025-08-20T03:09:47ZengKarger PublishersJournal of Innate Immunity1662-81282024-01-0116157359410.1159/000542078Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung TumorsZerick Terrell DunbarSalvador González-OchoaThanigaivelan KanagasabaiAlla IvanovaAnil Shanker Introduction: Natural killer (NK) cells are innate lymphoid cells capable of directly killing target cells while modulating immune effector responses. Despite their multifunctional capacities, a limited understanding of their plasticity and heterogeneity has impeded progress in developing effective NK cell-based cancer therapies. In this study, we investigated NK cell tissue heterogeneity in relation to their phenotype and effector functions against lung tumors. Methods: Using hanging drop tumor spheroid and subcutaneously established LL/2 (LLC1) lung tumor models, we examined NK cell receptor diversity and its correlation with tissue-specific cytotoxicity through multiparametric flow cytometry, fluorescence imaging, and cytotoxicity assays. Results: We identified distinct patterns of cell surface receptors expression on tissue-specific NK cells that are crucial for antitumor activity. Linear regression mathematical analyses further revealed significant positive correlations between activation-associated cell surface receptors and cytotoxic capacity in NK cells from tissues such as the liver and bone marrow. Conclusion: These findings underscore the differential effector capacities of NK cells from distinct tissues, even prior to exposure to LL/2 tumor cells. This highlights the significance of tissue-specific NK cell heterogeneity and its impact on their antitumor cytotoxicity. Recognizing these distinct tissue-specific receptor expression patterns will be instrumental in developing more efficacious NK cell-based cancer treatments. https://karger.com/article/doi/10.1159/000542078 |
| spellingShingle | Zerick Terrell Dunbar Salvador González-Ochoa Thanigaivelan Kanagasabai Alla Ivanova Anil Shanker Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung Tumors Journal of Innate Immunity |
| title | Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung Tumors |
| title_full | Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung Tumors |
| title_fullStr | Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung Tumors |
| title_full_unstemmed | Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung Tumors |
| title_short | Differential Effector Function of Tissue-Specific Natural Killer Cells against Lung Tumors |
| title_sort | differential effector function of tissue specific natural killer cells against lung tumors |
| url | https://karger.com/article/doi/10.1159/000542078 |
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