Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery Stenosis

<b>Objectives:</b> Recently, the need for early diagnosis of modifiable risk factors involved in the etiology of stroke has been highlighted in the literature. Nesfatin-1 is a peptide expressed in the central nervous system and peripheral tissues and has been used as a biomarker in recen...

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Main Authors: Şennur Delibaş Kati, Serkan Özben, Ertan Küçüksayan, Mert Van, Esra Yeğin Cilli, Aylin Yaman, Tomris Özben
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Diagnostics
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Online Access:https://www.mdpi.com/2075-4418/15/6/664
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author Şennur Delibaş Kati
Serkan Özben
Ertan Küçüksayan
Mert Van
Esra Yeğin Cilli
Aylin Yaman
Tomris Özben
author_facet Şennur Delibaş Kati
Serkan Özben
Ertan Küçüksayan
Mert Van
Esra Yeğin Cilli
Aylin Yaman
Tomris Özben
author_sort Şennur Delibaş Kati
collection DOAJ
description <b>Objectives:</b> Recently, the need for early diagnosis of modifiable risk factors involved in the etiology of stroke has been highlighted in the literature. Nesfatin-1 is a peptide expressed in the central nervous system and peripheral tissues and has been used as a biomarker in recent years. This study aimed to determine the association of ischemic stroke with internal carotid artery stenosis according to nesfatin-1 level and whether it could be used as a biomarker. <b>Methods:</b> A total of 118 patients were included in the study. Three groups were defined: acute stroke patients with symptomatic internal carotid artery stenosis, acute stroke patients without internal carotid artery stenosis, and a control group. Nesfatin-1 levels were measured and compared. <b>Results:</b> The median value was 22 pg/mL in acute stroke patients with internal carotid artery stenosis, 24.3 pg/mL in acute stroke patients without internal carotid artery stenosis, and 46.4 pg/mL in the control group. There is a difference between the median values of nesfatin-1 according to the stroke groups with the control group (<i>p</i> < 0.001). When a cut-off value of ≤30.62 was taken for nesfatin-1, an AUC value of 0.773 indicated statistical significance (<i>p</i> < 0.001). Sensitivity was 77.03%, specificity 83.33%, PPV 90.48%, and NPV 63.83%. The main limitations of our study are the small sample size and the fact that the function of nesfatin-1 is not completely known. <b>Conclusions:</b> Although we found that nesfatin-1 levels were lower in ischemic stroke patients compared to controls, its diagnostic potential indicates a moderate discriminatory ability with an AUC value of 0.773. Therefore, whether it is suitable for clinical use will be demonstrated by studies in larger and multicenter cohorts.
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spelling doaj-art-13eaaf6cbad047319f8ffc738793428b2025-08-20T03:43:36ZengMDPI AGDiagnostics2075-44182025-03-0115666410.3390/diagnostics15060664Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery StenosisŞennur Delibaş Kati0Serkan Özben1Ertan Küçüksayan2Mert Van3Esra Yeğin Cilli4Aylin Yaman5Tomris Özben6Department of Neurology, Antalya Health Research Center, University of Health Sciences, 07100 Antalya, TürkiyeDepartment of Neurology, Antalya Health Research Center, University of Health Sciences, 07100 Antalya, TürkiyeDepartment of Biochemistry, Faculty of Medicine, Alanya Alaaddin Keykubat University, 07425 Antalya, TürkiyeDepartment of Neurology, Antalya Health Research Center, University of Health Sciences, 07100 Antalya, TürkiyeDepartment of Neurology, Antalya Korkuteli State Hospital, 07800 Antalya, TürkiyeDepartment of Neurology, Antalya Health Research Center, University of Health Sciences, 07100 Antalya, TürkiyeDepartment of Medical Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Türkiye<b>Objectives:</b> Recently, the need for early diagnosis of modifiable risk factors involved in the etiology of stroke has been highlighted in the literature. Nesfatin-1 is a peptide expressed in the central nervous system and peripheral tissues and has been used as a biomarker in recent years. This study aimed to determine the association of ischemic stroke with internal carotid artery stenosis according to nesfatin-1 level and whether it could be used as a biomarker. <b>Methods:</b> A total of 118 patients were included in the study. Three groups were defined: acute stroke patients with symptomatic internal carotid artery stenosis, acute stroke patients without internal carotid artery stenosis, and a control group. Nesfatin-1 levels were measured and compared. <b>Results:</b> The median value was 22 pg/mL in acute stroke patients with internal carotid artery stenosis, 24.3 pg/mL in acute stroke patients without internal carotid artery stenosis, and 46.4 pg/mL in the control group. There is a difference between the median values of nesfatin-1 according to the stroke groups with the control group (<i>p</i> < 0.001). When a cut-off value of ≤30.62 was taken for nesfatin-1, an AUC value of 0.773 indicated statistical significance (<i>p</i> < 0.001). Sensitivity was 77.03%, specificity 83.33%, PPV 90.48%, and NPV 63.83%. The main limitations of our study are the small sample size and the fact that the function of nesfatin-1 is not completely known. <b>Conclusions:</b> Although we found that nesfatin-1 levels were lower in ischemic stroke patients compared to controls, its diagnostic potential indicates a moderate discriminatory ability with an AUC value of 0.773. Therefore, whether it is suitable for clinical use will be demonstrated by studies in larger and multicenter cohorts.https://www.mdpi.com/2075-4418/15/6/664strokebiomarkerinternal carotid arterystenosisnesfatin-1
spellingShingle Şennur Delibaş Kati
Serkan Özben
Ertan Küçüksayan
Mert Van
Esra Yeğin Cilli
Aylin Yaman
Tomris Özben
Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery Stenosis
Diagnostics
stroke
biomarker
internal carotid artery
stenosis
nesfatin-1
title Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery Stenosis
title_full Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery Stenosis
title_fullStr Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery Stenosis
title_full_unstemmed Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery Stenosis
title_short Nesfatin-1 as a Potential Biomarker for Ischemic Stroke: A Case-Controlled Study of a Comparative Analysis of Patients with and Without Internal Carotid Artery Stenosis
title_sort nesfatin 1 as a potential biomarker for ischemic stroke a case controlled study of a comparative analysis of patients with and without internal carotid artery stenosis
topic stroke
biomarker
internal carotid artery
stenosis
nesfatin-1
url https://www.mdpi.com/2075-4418/15/6/664
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