Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism
ABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissu...
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| Series: | Arquivos de Neuro-Psiquiatria |
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| _version_ | 1849308114900221952 |
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| author | Cristiane Iozzi Silva Paulo Cézar Novais Andressa Romualdo Rodrigues Camila A.M. Carvalho Benedicto Oscar Colli Carlos Gilberto Carlotti Jr. Luís Fernando Tirapelli Daniela P.C. Tirapelli |
| author_facet | Cristiane Iozzi Silva Paulo Cézar Novais Andressa Romualdo Rodrigues Camila A.M. Carvalho Benedicto Oscar Colli Carlos Gilberto Carlotti Jr. Luís Fernando Tirapelli Daniela P.C. Tirapelli |
| author_sort | Cristiane Iozzi Silva |
| collection | DOAJ |
| description | ABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them. |
| format | Article |
| id | doaj-art-13e586cdb84c4c469fa83d0218d3d826 |
| institution | Kabale University |
| issn | 1678-4227 |
| language | English |
| publisher | Thieme Revinter Publicações |
| record_format | Article |
| series | Arquivos de Neuro-Psiquiatria |
| spelling | doaj-art-13e586cdb84c4c469fa83d0218d3d8262025-08-20T03:54:33ZengThieme Revinter PublicaçõesArquivos de Neuro-Psiquiatria1678-4227751303510.1590/0004-282x20160188S0004-282X2017000100030Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholismCristiane Iozzi SilvaPaulo Cézar NovaisAndressa Romualdo RodriguesCamila A.M. CarvalhoBenedicto Oscar ColliCarlos Gilberto Carlotti Jr.Luís Fernando TirapelliDaniela P.C. TirapelliABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2017000100030&lng=en&tlng=enisquemia encefálicaalcoolismoN-MetilaspartatomicroRNAs |
| spellingShingle | Cristiane Iozzi Silva Paulo Cézar Novais Andressa Romualdo Rodrigues Camila A.M. Carvalho Benedicto Oscar Colli Carlos Gilberto Carlotti Jr. Luís Fernando Tirapelli Daniela P.C. Tirapelli Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism Arquivos de Neuro-Psiquiatria isquemia encefálica alcoolismo N-Metilaspartato microRNAs |
| title | Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
| title_full | Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
| title_fullStr | Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
| title_full_unstemmed | Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
| title_short | Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism |
| title_sort | expression of nmda receptor and microrna 219 in rats submitted to cerebral ischemia associated with alcoholism |
| topic | isquemia encefálica alcoolismo N-Metilaspartato microRNAs |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2017000100030&lng=en&tlng=en |
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