Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey
The rate of in vitro resistance to various antimicrobials in 179 consecutive isolates of Salmonella spp., which included serogroups D (109), B (52), C1 (10) and C2 (8) isolated from children, was investigated. Production of extended-spectrum beta-lactamase (ESBL) was studied in ampicillin-res...
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| Format: | Article |
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Hacettepe University Institute of Child Health
2009-02-01
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| Series: | The Turkish Journal of Pediatrics |
| Online Access: | https://turkjpediatr.org/article/view/2253 |
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| author | Fatma Budak Patrice Nordmann Delphine Girlich Deniz Gür |
| author_facet | Fatma Budak Patrice Nordmann Delphine Girlich Deniz Gür |
| author_sort | Fatma Budak |
| collection | DOAJ |
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The rate of in vitro resistance to various antimicrobials in 179 consecutive isolates of Salmonella spp., which included serogroups D (109), B (52), C1 (10) and C2 (8) isolated from children, was investigated. Production of extended-spectrum beta-lactamase (ESBL) was studied in ampicillin-resistant isolates. Antimicrobial susceptibilities were determined by disk diffusion tests and by BIOMIC video reader system. Overall resistance rates to ampicillin and amoxicillin/clavulanate were 26.3% and 10.6%, respectively. Resistance to ceftriaxone and ceftazidime was 3.3%. Resistance rates for chloramphenicol, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin and gentamicin were 40.7%, 31.3%, 2.2%, 2.2% and 6.1%, respectively. beta-lactamase production was detected in 42 isolates. Mating out experiments, isoelectric focusing, dot blot hybridization, polymerase chain reaction (PCR) and sequencing were performed on two S. paratyphi B isolates that produced ESBLs. One isolate produced SHV-2 and TEM-1 and the other produced SHV-2a, SHV-5a (SHV-9) and TEM-1. This is the first report of SHV-2a and SHV-5a (SHV-9) in S. paratyphi B in Turkey.
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| format | Article |
| id | doaj-art-13e38694fba84f0e81cf3f60edc8b9eb |
| institution | DOAJ |
| issn | 0041-4301 2791-6421 |
| language | English |
| publishDate | 2009-02-01 |
| publisher | Hacettepe University Institute of Child Health |
| record_format | Article |
| series | The Turkish Journal of Pediatrics |
| spelling | doaj-art-13e38694fba84f0e81cf3f60edc8b9eb2025-08-20T02:55:39ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212009-02-01511Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from TurkeyFatma Budak0Patrice NordmannDelphine GirlichDeniz GürDepartment of Microbiology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey. The rate of in vitro resistance to various antimicrobials in 179 consecutive isolates of Salmonella spp., which included serogroups D (109), B (52), C1 (10) and C2 (8) isolated from children, was investigated. Production of extended-spectrum beta-lactamase (ESBL) was studied in ampicillin-resistant isolates. Antimicrobial susceptibilities were determined by disk diffusion tests and by BIOMIC video reader system. Overall resistance rates to ampicillin and amoxicillin/clavulanate were 26.3% and 10.6%, respectively. Resistance to ceftriaxone and ceftazidime was 3.3%. Resistance rates for chloramphenicol, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin and gentamicin were 40.7%, 31.3%, 2.2%, 2.2% and 6.1%, respectively. beta-lactamase production was detected in 42 isolates. Mating out experiments, isoelectric focusing, dot blot hybridization, polymerase chain reaction (PCR) and sequencing were performed on two S. paratyphi B isolates that produced ESBLs. One isolate produced SHV-2 and TEM-1 and the other produced SHV-2a, SHV-5a (SHV-9) and TEM-1. This is the first report of SHV-2a and SHV-5a (SHV-9) in S. paratyphi B in Turkey. https://turkjpediatr.org/article/view/2253 |
| spellingShingle | Fatma Budak Patrice Nordmann Delphine Girlich Deniz Gür Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey The Turkish Journal of Pediatrics |
| title | Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey |
| title_full | Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey |
| title_fullStr | Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey |
| title_full_unstemmed | Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey |
| title_short | Characterization of extended-spectrum beta-lactamase-producing Salmonella isolates in a children's hospital in Ankara--first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey |
| title_sort | characterization of extended spectrum beta lactamase producing salmonella isolates in a children s hospital in ankara first report of shv 2a and shv 9 in salmonella spp from turkey |
| url | https://turkjpediatr.org/article/view/2253 |
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