Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage?
Liver grafts suffer from unavoidable injury due to ischemia and manipulation before implantation. Danger signals such as high-mobility group box -1(HMGB1) and macrophage migration inhibitory factor (MIF) play a pivotal role in the immune response. We characterized the kinetics of their release into...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2010/436145 |
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| _version_ | 1849308113429069824 |
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| author | Anding Liu Hao Jin Olaf Dirsch Meihong Deng Hai Huang Martina Bröcker-Preuss Uta Dahmen |
| author_facet | Anding Liu Hao Jin Olaf Dirsch Meihong Deng Hai Huang Martina Bröcker-Preuss Uta Dahmen |
| author_sort | Anding Liu |
| collection | DOAJ |
| description | Liver grafts suffer from unavoidable injury due to ischemia and manipulation before implantation. Danger signals such as high-mobility group box -1(HMGB1) and macrophage migration inhibitory factor (MIF) play a pivotal role in the immune response. We characterized the kinetics of their release into the effluent during cold/warm ischemia and additional manipulation-induced mechanical damage. Furthermore, we evaluated the relationship between HMGB1/MIF release and ischemic/mechanical damage. Liver enzymes and protein in the effluent increased with increasing ischemia time. HMGB1/MIF- release correlated with the extent of hepatocellular injury. With increasing ischemia time and damage, HMGB1 was translocated from the nucleus to the cytoplasma as indicated by weak nuclear and strong cytoplasmic staining. Enhancement of liver injury by mechanical damage was indicated by an earlier HMGB1 translocation into the cytoplasm and earlier release of danger signals into the effluent. Our results suggest that determination of HMGB1 and MIF reflects the extent of ischemic injury. Furthermore, HMGB1and MIF are more sensitive than liver enzymes to detect the additional mechanical damage inflicted on the organ graft during surgical manipulation. |
| format | Article |
| id | doaj-art-13dda38d8f024da89fa721a76ffebb3a |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-13dda38d8f024da89fa721a76ffebb3a2025-08-20T03:54:33ZengWileyMediators of Inflammation0962-93511466-18612010-01-01201010.1155/2010/436145436145Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage?Anding Liu0Hao Jin1Olaf Dirsch2Meihong Deng3Hai Huang4Martina Bröcker-Preuss5Uta Dahmen6Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Friedrich Schiller University Jena, Drackendorfer Str.1, 07747 Jena, GermanyExperimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Friedrich Schiller University Jena, Drackendorfer Str.1, 07747 Jena, GermanyInstitute of Pathology, University Hospital Jena, 07747 Jena, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Essen, University of Duisburg and Essen, 45122 Essen, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Essen, University of Duisburg and Essen, 45122 Essen, GermanyDepartment of Clinical Chemistry, Clinic of Endocrinology, University Hospital Essen, University of Duisburg and Essen, 45122 Essen, GermanyExperimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Friedrich Schiller University Jena, Drackendorfer Str.1, 07747 Jena, GermanyLiver grafts suffer from unavoidable injury due to ischemia and manipulation before implantation. Danger signals such as high-mobility group box -1(HMGB1) and macrophage migration inhibitory factor (MIF) play a pivotal role in the immune response. We characterized the kinetics of their release into the effluent during cold/warm ischemia and additional manipulation-induced mechanical damage. Furthermore, we evaluated the relationship between HMGB1/MIF release and ischemic/mechanical damage. Liver enzymes and protein in the effluent increased with increasing ischemia time. HMGB1/MIF- release correlated with the extent of hepatocellular injury. With increasing ischemia time and damage, HMGB1 was translocated from the nucleus to the cytoplasma as indicated by weak nuclear and strong cytoplasmic staining. Enhancement of liver injury by mechanical damage was indicated by an earlier HMGB1 translocation into the cytoplasm and earlier release of danger signals into the effluent. Our results suggest that determination of HMGB1 and MIF reflects the extent of ischemic injury. Furthermore, HMGB1and MIF are more sensitive than liver enzymes to detect the additional mechanical damage inflicted on the organ graft during surgical manipulation.http://dx.doi.org/10.1155/2010/436145 |
| spellingShingle | Anding Liu Hao Jin Olaf Dirsch Meihong Deng Hai Huang Martina Bröcker-Preuss Uta Dahmen Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage? Mediators of Inflammation |
| title | Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage? |
| title_full | Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage? |
| title_fullStr | Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage? |
| title_full_unstemmed | Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage? |
| title_short | Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage? |
| title_sort | release of danger signals during ischemic storage of the liver a potential marker of organ damage |
| url | http://dx.doi.org/10.1155/2010/436145 |
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