Establishment & characterization of a non-adherent insect cell line for cultivated meat
Abstract This study presents a blueprint for developing, scaling, and analyzing novel insect cell lines for food. The large-scale production of cultivated meat requires the development and analysis of cell lines that are simple to grow and easy to scale. Insect cells may be a favorable cell source d...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-86921-z |
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| author | Sophia M. Letcher Olivia P. Calkins Halla J. Clausi Aidan McCreary Barry A. Trimmer David L. Kaplan |
| author_facet | Sophia M. Letcher Olivia P. Calkins Halla J. Clausi Aidan McCreary Barry A. Trimmer David L. Kaplan |
| author_sort | Sophia M. Letcher |
| collection | DOAJ |
| description | Abstract This study presents a blueprint for developing, scaling, and analyzing novel insect cell lines for food. The large-scale production of cultivated meat requires the development and analysis of cell lines that are simple to grow and easy to scale. Insect cells may be a favorable cell source due to their robust growth properties, adaptability to different culture conditions, and resiliency in culture. Cells were isolated from Tobacco hornworm (Manduca sexta) embryos and subsequently adapted to single-cell suspension culture in animal-free growth media. Cells were able to reach relatively high cell densities of over 20 million cells per mL in shake flasks. Cell growth data is presented in various culture vessels and spent media analysis was performed to better understand cell metabolic processes. Finally, a preliminary nutritional profile consisting of proximate, amino acid, mineral, and fatty acid analysis is reported. |
| format | Article |
| id | doaj-art-13cd75a63a6942aab093da7d4de596d5 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-13cd75a63a6942aab093da7d4de596d52025-08-20T03:05:56ZengNature PortfolioScientific Reports2045-23222025-03-0115111710.1038/s41598-025-86921-zEstablishment & characterization of a non-adherent insect cell line for cultivated meatSophia M. Letcher0Olivia P. Calkins1Halla J. Clausi2Aidan McCreary3Barry A. Trimmer4David L. Kaplan5Department of Biomedical Engineering, Tufts UniversityDepartment of Chemical Engineering, Tufts UniversityDepartment of Biomedical Engineering, Tufts UniversityDepartment of Chemistry, Tufts UniversityDepartment of Biomedical Engineering, Tufts UniversityDepartment of Biomedical Engineering, Tufts UniversityAbstract This study presents a blueprint for developing, scaling, and analyzing novel insect cell lines for food. The large-scale production of cultivated meat requires the development and analysis of cell lines that are simple to grow and easy to scale. Insect cells may be a favorable cell source due to their robust growth properties, adaptability to different culture conditions, and resiliency in culture. Cells were isolated from Tobacco hornworm (Manduca sexta) embryos and subsequently adapted to single-cell suspension culture in animal-free growth media. Cells were able to reach relatively high cell densities of over 20 million cells per mL in shake flasks. Cell growth data is presented in various culture vessels and spent media analysis was performed to better understand cell metabolic processes. Finally, a preliminary nutritional profile consisting of proximate, amino acid, mineral, and fatty acid analysis is reported.https://doi.org/10.1038/s41598-025-86921-zInsect cell cultureCellular agricultureCultured meatCultivated meatNutritionSuspension culture |
| spellingShingle | Sophia M. Letcher Olivia P. Calkins Halla J. Clausi Aidan McCreary Barry A. Trimmer David L. Kaplan Establishment & characterization of a non-adherent insect cell line for cultivated meat Scientific Reports Insect cell culture Cellular agriculture Cultured meat Cultivated meat Nutrition Suspension culture |
| title | Establishment & characterization of a non-adherent insect cell line for cultivated meat |
| title_full | Establishment & characterization of a non-adherent insect cell line for cultivated meat |
| title_fullStr | Establishment & characterization of a non-adherent insect cell line for cultivated meat |
| title_full_unstemmed | Establishment & characterization of a non-adherent insect cell line for cultivated meat |
| title_short | Establishment & characterization of a non-adherent insect cell line for cultivated meat |
| title_sort | establishment characterization of a non adherent insect cell line for cultivated meat |
| topic | Insect cell culture Cellular agriculture Cultured meat Cultivated meat Nutrition Suspension culture |
| url | https://doi.org/10.1038/s41598-025-86921-z |
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