High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of Morocco
Point-of-care (POC) testing for Toxoplasma infection has the potential to revolutionize diagnosis and management of toxoplasmosis, especially in high-risk populations in areas with significant environmental contamination and poor health infrastructure precluding appropriate follow-up and preventing...
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1948359 |
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| author | Bouchra El Mansouri Fatima Amarir François Peyron El Bachir Adlaoui Raphaël Piarroux Joseph Lykins Majda El Abbassi Nesma Nekkal Nadia Bouhlal Kamar Makkaoui Amina Barkat Aziza Lyaghfouri Ying Zhou Samira Rais Mounia Oudghiri Ismail Elkoraichi Mustapha Zekri Nezha Belkadi Hajar Mellouk Mohamed Rhajaoui Allal Boutajangout Abderrahim Sadak Denis Limonne Rima McLeod Kamal El Bissati |
| author_facet | Bouchra El Mansouri Fatima Amarir François Peyron El Bachir Adlaoui Raphaël Piarroux Joseph Lykins Majda El Abbassi Nesma Nekkal Nadia Bouhlal Kamar Makkaoui Amina Barkat Aziza Lyaghfouri Ying Zhou Samira Rais Mounia Oudghiri Ismail Elkoraichi Mustapha Zekri Nezha Belkadi Hajar Mellouk Mohamed Rhajaoui Allal Boutajangout Abderrahim Sadak Denis Limonne Rima McLeod Kamal El Bissati |
| author_sort | Bouchra El Mansouri |
| collection | DOAJ |
| description | Point-of-care (POC) testing for Toxoplasma infection has the potential to revolutionize diagnosis and management of toxoplasmosis, especially in high-risk populations in areas with significant environmental contamination and poor health infrastructure precluding appropriate follow-up and preventing access to medical care. Toxoplasmosis is a significant public health challenge in Morocco, with a relatively heavy burden of infection and, to this point, minimal investment nationally to address this infection. Herein, we analyse the performance of a novel, low-cost rapid test using fingerstick-derived whole blood from 632 women (82 of whom were pregnant) from slums, educational centres, and from nomad groups across different geographical regions (i.e. oceanic, mountainous) of Morocco. The POC test was highly sensitive and specific from all settings. In the first group of 283 women, sera were tested by Platelia ELISA IgG and IgM along with fingerstick whole blood test. Then a matrix study with 349 women was performed in which fingerstick – POC test results and serum obtained by venipuncture contemporaneously were compared. These results show high POC test performance (Sensitivity: 96.4% [IC95 90.6–98.9%]; Specificity: 99.6% [IC95 97.3–99.9%]) and high prevalence of Toxoplasma infection among women living in rural and mountainous areas, and in urban areas with lower educational levels. The high performance of POC test confirms that it can reduce the need for venipuncture and clinical infrastructure in a low-resource setting. It can be used to efficiently perform seroprevalence determinations in large group settings across a range of demographics, and potentially expands healthcare access, thereby preventing human suffering. |
| format | Article |
| id | doaj-art-13c8feade134462593521bbec4a73f12 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-13c8feade134462593521bbec4a73f122025-08-20T01:49:27ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-011011675168210.1080/22221751.2021.1948359High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of MoroccoBouchra El Mansouri0Fatima Amarir1François Peyron2El Bachir Adlaoui3Raphaël Piarroux4Joseph Lykins5Majda El Abbassi6Nesma Nekkal7Nadia Bouhlal8Kamar Makkaoui9Amina Barkat10Aziza Lyaghfouri11Ying Zhou12Samira Rais13Mounia Oudghiri14Ismail Elkoraichi15Mustapha Zekri16Nezha Belkadi17Hajar Mellouk18Mohamed Rhajaoui19Allal Boutajangout20Abderrahim Sadak21Denis Limonne22Rima McLeod23Kamal El Bissati24Institut National d’Hygiène, Rabat, MoroccoLaboratory of Immunity and Biodiversity, Department of Biology, Faculty of Sciences Aïn Chock, Hassan II University, Casablanca, MoroccoInstitut de Parasitologie et de Mycologie Médicale Hôpital de la Croix Rousse, Lyon, FranceInstitut National d’Hygiène, Rabat, MoroccoLDBIO Diagnostics, Lyon, FranceDepartment of Internal Medicine, Virginia Commonwealth University Health System, Richmond, VA, USAInstitut National d’Hygiène, Rabat, MoroccoMohamed VI Polytechnic University, CIAM, Benguerir, MoroccoService de Réseaux des Etablissements Santaires (SRES), Tinghir, MoroccoThe Toxoplasmosis Study Group, Obstetrics and Gynecology, Rabat, MoroccoResearch Team on Mother-Child Health and Nutrition, Faculty of Medicine & Pharmacy, Mohamed V University, Rabat, MoroccoService de la Protection de la Santé Infantile, Direction de la Population, Rabat, MoroccoDepartment of Ophthalmology and Visual Sciences, Pediatrics (Infectious Diseases), Global Health Center, Toxoplasmosis Center, University of Chicago Medical Center, Chicago, IL, USALaboratory of Immunity and Biodiversity, Department of Biology, Faculty of Sciences Aïn Chock, Hassan II University, Casablanca, MoroccoLaboratory of Immunity and Biodiversity, Department of Biology, Faculty of Sciences Aïn Chock, Hassan II University, Casablanca, MoroccoLaboratory of Immunity and Biodiversity, Department of Biology, Faculty of Sciences Aïn Chock, Hassan II University, Casablanca, MoroccoHigh Institute for Nursing Professions and Health Techniques (ISPITS), Casablanca, MoroccoHigh Institute for Nursing Professions and Health Techniques (ISPITS), Casablanca, MoroccoHigh Institute for Nursing Professions and Health Techniques (ISPITS), Casablanca, MoroccoInstitut National d’Hygiène, Rabat, MoroccoDepartment of Neurology & Physiology and Neuroscience, New York University Langone Health, New York, USAFaculty of Sciences, Laboratory of Zoology and General Biology, Rabat, MoroccoLDBIO Diagnostics, Lyon, FranceDepartment of Ophthalmology and Visual Sciences, Pediatrics (Infectious Diseases), Global Health Center, Toxoplasmosis Center, University of Chicago Medical Center, Chicago, IL, USAInstitute of Molecular Engineering, University of Chicago Medical Center, Chicago, IL, USAPoint-of-care (POC) testing for Toxoplasma infection has the potential to revolutionize diagnosis and management of toxoplasmosis, especially in high-risk populations in areas with significant environmental contamination and poor health infrastructure precluding appropriate follow-up and preventing access to medical care. Toxoplasmosis is a significant public health challenge in Morocco, with a relatively heavy burden of infection and, to this point, minimal investment nationally to address this infection. Herein, we analyse the performance of a novel, low-cost rapid test using fingerstick-derived whole blood from 632 women (82 of whom were pregnant) from slums, educational centres, and from nomad groups across different geographical regions (i.e. oceanic, mountainous) of Morocco. The POC test was highly sensitive and specific from all settings. In the first group of 283 women, sera were tested by Platelia ELISA IgG and IgM along with fingerstick whole blood test. Then a matrix study with 349 women was performed in which fingerstick – POC test results and serum obtained by venipuncture contemporaneously were compared. These results show high POC test performance (Sensitivity: 96.4% [IC95 90.6–98.9%]; Specificity: 99.6% [IC95 97.3–99.9%]) and high prevalence of Toxoplasma infection among women living in rural and mountainous areas, and in urban areas with lower educational levels. The high performance of POC test confirms that it can reduce the need for venipuncture and clinical infrastructure in a low-resource setting. It can be used to efficiently perform seroprevalence determinations in large group settings across a range of demographics, and potentially expands healthcare access, thereby preventing human suffering.https://www.tandfonline.com/doi/10.1080/22221751.2021.1948359Congenital toxoplasmosisPOCscreeningLDBIO testdiagnostics |
| spellingShingle | Bouchra El Mansouri Fatima Amarir François Peyron El Bachir Adlaoui Raphaël Piarroux Joseph Lykins Majda El Abbassi Nesma Nekkal Nadia Bouhlal Kamar Makkaoui Amina Barkat Aziza Lyaghfouri Ying Zhou Samira Rais Mounia Oudghiri Ismail Elkoraichi Mustapha Zekri Nezha Belkadi Hajar Mellouk Mohamed Rhajaoui Allal Boutajangout Abderrahim Sadak Denis Limonne Rima McLeod Kamal El Bissati High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of Morocco Emerging Microbes and Infections Congenital toxoplasmosis POC screening LDBIO test diagnostics |
| title | High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of Morocco |
| title_full | High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of Morocco |
| title_fullStr | High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of Morocco |
| title_full_unstemmed | High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of Morocco |
| title_short | High performance of a novel point-of-care blood test for Toxoplasma infection in women from diverse regions of Morocco |
| title_sort | high performance of a novel point of care blood test for toxoplasma infection in women from diverse regions of morocco |
| topic | Congenital toxoplasmosis POC screening LDBIO test diagnostics |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1948359 |
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