Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique
The rapid diagnosis of bovine tuberculosis (BT) allows official surveillance institutions to act in areas that could be potential hotspots for the spread of the disease. The study aimed to identify the Mycobacterium bovis agent by multiplex PCR of isolated colonies. Through the official inspection...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Goiás
2024-10-01
|
| Series: | Ciência Animal Brasileira |
| Online Access: | https://revistas.ufg.br/vet/article/view/78409 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850184458832969728 |
|---|---|
| author | Bruno Ribeiro dos Santos Bianca Mendes Maciel Alana Venâncio da Silva Hllytchaikra Ferraz Fehlberg Fernando Alzamora Filho |
| author_facet | Bruno Ribeiro dos Santos Bianca Mendes Maciel Alana Venâncio da Silva Hllytchaikra Ferraz Fehlberg Fernando Alzamora Filho |
| author_sort | Bruno Ribeiro dos Santos |
| collection | DOAJ |
| description |
The rapid diagnosis of bovine tuberculosis (BT) allows official surveillance institutions to act
in areas that could be potential hotspots for the spread of the disease. The study aimed to identify
the Mycobacterium bovis agent by multiplex PCR of isolated colonies. Through the official inspection
service (Federal/State), samples suggestive of BT were collected during the post-mortem inspection
of bovine carcasses in slaughterhouses and sent to the laboratory for complementary diagnostics.
The samples were analyzed using bacteriological culture and Ziehl-Neelsen staining. In isolates
characterized as Acid-Alcohol Resistant Bacilli (BAAR), the multiplex PCR technique confirmed the agent
Mycobacterium bovis. Nine hundred ninety-one thousand three hundred sixteen cattle carcasses
were inspected, collecting 32 BT-suggestive samples in from animalsfrom 30 municipalities. Of these,
28.1% (9/32) showed growth on bacteriological culture media with BAAR. The BAAR isolates submitted
to multiplex PCR confirmed the presence of M. bovis. The multiplex PCR technique associated with
bacteriological examination and applied to post-mortem findings enabled the diagnosis of M. bovis in
the municipalities sampled in the Bahia state.
|
| format | Article |
| id | doaj-art-13bda39ea29045599f9544ed448b2b28 |
| institution | OA Journals |
| issn | 1518-2797 1809-6891 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj-art-13bda39ea29045599f9544ed448b2b282025-08-20T02:17:01ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912024-10-0125Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR techniqueBruno Ribeiro dos Santos 0Bianca Mendes Maciel1Alana Venâncio da Silva2Hllytchaikra Ferraz Fehlberg3Fernando Alzamora Filho4Universidade Estadual de Santa Cruz (UESC), Ilheus, Bahia, BrasilUniversidade Estadual de Santa Cruz (UESC), Ilheus, Bahia, BrasilUniversidade Estadual de Santa Cruz (UESC), Ilheus, Bahia, BrasilUniversidade Estadual de Santa Cruz (UESC), Ilheus, Bahia, BrasilUniversidade Estadual de Santa Cruz (UESC), Ilheus, Bahia, Brasil The rapid diagnosis of bovine tuberculosis (BT) allows official surveillance institutions to act in areas that could be potential hotspots for the spread of the disease. The study aimed to identify the Mycobacterium bovis agent by multiplex PCR of isolated colonies. Through the official inspection service (Federal/State), samples suggestive of BT were collected during the post-mortem inspection of bovine carcasses in slaughterhouses and sent to the laboratory for complementary diagnostics. The samples were analyzed using bacteriological culture and Ziehl-Neelsen staining. In isolates characterized as Acid-Alcohol Resistant Bacilli (BAAR), the multiplex PCR technique confirmed the agent Mycobacterium bovis. Nine hundred ninety-one thousand three hundred sixteen cattle carcasses were inspected, collecting 32 BT-suggestive samples in from animalsfrom 30 municipalities. Of these, 28.1% (9/32) showed growth on bacteriological culture media with BAAR. The BAAR isolates submitted to multiplex PCR confirmed the presence of M. bovis. The multiplex PCR technique associated with bacteriological examination and applied to post-mortem findings enabled the diagnosis of M. bovis in the municipalities sampled in the Bahia state. https://revistas.ufg.br/vet/article/view/78409 |
| spellingShingle | Bruno Ribeiro dos Santos Bianca Mendes Maciel Alana Venâncio da Silva Hllytchaikra Ferraz Fehlberg Fernando Alzamora Filho Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique Ciência Animal Brasileira |
| title | Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique |
| title_full | Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique |
| title_fullStr | Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique |
| title_full_unstemmed | Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique |
| title_short | Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique |
| title_sort | bovine tuberculosis diagnosis in the state of bahia brazil using the multiplex pcr technique |
| url | https://revistas.ufg.br/vet/article/view/78409 |
| work_keys_str_mv | AT brunoribeirodossantos bovinetuberculosisdiagnosisinthestateofbahiabrazilusingthemultiplexpcrtechnique AT biancamendesmaciel bovinetuberculosisdiagnosisinthestateofbahiabrazilusingthemultiplexpcrtechnique AT alanavenanciodasilva bovinetuberculosisdiagnosisinthestateofbahiabrazilusingthemultiplexpcrtechnique AT hllytchaikraferrazfehlberg bovinetuberculosisdiagnosisinthestateofbahiabrazilusingthemultiplexpcrtechnique AT fernandoalzamorafilho bovinetuberculosisdiagnosisinthestateofbahiabrazilusingthemultiplexpcrtechnique |