Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population that primarily suppress T lymphocytes in healthy pregnancies and pathologies. MDSCs are one of the key regulators of immune responses. Finding ways to control them is important for the treatment of cancer, autoimmune diseas...
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2023-06-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/2838 |
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| author | V. P. Timganova K. Yu. Shardina M. S. Bochkova S. V. Uzhviyuk D. I. Usanina S. A. Zamorina |
| author_facet | V. P. Timganova K. Yu. Shardina M. S. Bochkova S. V. Uzhviyuk D. I. Usanina S. A. Zamorina |
| author_sort | V. P. Timganova |
| collection | DOAJ |
| description | Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population that primarily suppress T lymphocytes in healthy pregnancies and pathologies. MDSCs are one of the key regulators of immune responses. Finding ways to control them is important for the treatment of cancer, autoimmune diseases, miscarriage, and post-transplant complications. The mechanisms of immune suppression by MDSC are: expression of CD73, ADAM17, PD -L1, production of Arg 1, iNOS, IDO, IL -10 and TGF-b1.Pregnancy-specific b1-glycoprotein (PSG) has modulatory effects on dendritic cells and macrophages that mediate the shift of T cell phenotypes toward Th2 and Treg. We have previously shown that native PSG suppresses Th17 differentiation and cytokine production, stimulates the production of IDO by monocytes and the differentiation of Tregs.Considering the immunomodulatory properties of PSG and the key role of MDSCs in pathologies, the aim of our work was to investigate the effect of native and recombinant PSG on the differentiation of MDSCs in vitro.MDSCs were differentiated from CD11b+ peripheral blood cells. Cells were cultured for 7 days and received stepwise GM-CSF, IL-1b, and LPS. Native (n) (1; 10 and 100 mg/mL) and recombinant (r) (1 and 10 mg/mL) PSG were introduced into the cultures three days before the end of incubation. Flow cytometry was used to determine the percentage of MDSC among the cells in culture and the percentage of M-, PMN-, and e-MDSC among the total number of MDSCs.It was found that rPSG (1 mg/mL) increased the percentage of MDSCs in culture. Both nPSG (1 and 10 mg/mL) and rPSG (10 mg/mL) increased the proportion of M-MDSC, whereas rPSG (10 mg/mL) decreased the number of PMN-MDSC.Thus, the cytokine background in CD11b+ cell cultures favored the differentiation of predominantly M-MDSC, similar to the tumor microenvironment, whereas native and recombinant PSG enhanced this effect. Thus, nPSG and rPSG are able to modulate the differentiation of MDSCs by increasing their number, mainly due to the monocytic subpopulation. This fact opens perspectives for new research on targeted manipulation of MDSCs. |
| format | Article |
| id | doaj-art-13b45f1f6bbe4fd482b7708913503380 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2023-06-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-13b45f1f6bbe4fd482b77089135033802025-08-20T03:37:57ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2023-06-0125351352010.15789/1563-0625-EOP-28381691Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiationV. P. Timganova0K. Yu. Shardina1M. S. Bochkova2S. V. Uzhviyuk3D. I. Usanina4S. A. Zamorina5Institute of Ecology and Genetics of Microorganisms, Branch of the Perm Federal Research Center, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Branch of the Perm Federal Research Center, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Branch of the Perm Federal Research Center, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Branch of the Perm Federal Research Center, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Branch of the Perm Federal Research Center, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Branch of the Perm Federal Research Center, Ural Branch, Russian Academy of SciencesMyeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population that primarily suppress T lymphocytes in healthy pregnancies and pathologies. MDSCs are one of the key regulators of immune responses. Finding ways to control them is important for the treatment of cancer, autoimmune diseases, miscarriage, and post-transplant complications. The mechanisms of immune suppression by MDSC are: expression of CD73, ADAM17, PD -L1, production of Arg 1, iNOS, IDO, IL -10 and TGF-b1.Pregnancy-specific b1-glycoprotein (PSG) has modulatory effects on dendritic cells and macrophages that mediate the shift of T cell phenotypes toward Th2 and Treg. We have previously shown that native PSG suppresses Th17 differentiation and cytokine production, stimulates the production of IDO by monocytes and the differentiation of Tregs.Considering the immunomodulatory properties of PSG and the key role of MDSCs in pathologies, the aim of our work was to investigate the effect of native and recombinant PSG on the differentiation of MDSCs in vitro.MDSCs were differentiated from CD11b+ peripheral blood cells. Cells were cultured for 7 days and received stepwise GM-CSF, IL-1b, and LPS. Native (n) (1; 10 and 100 mg/mL) and recombinant (r) (1 and 10 mg/mL) PSG were introduced into the cultures three days before the end of incubation. Flow cytometry was used to determine the percentage of MDSC among the cells in culture and the percentage of M-, PMN-, and e-MDSC among the total number of MDSCs.It was found that rPSG (1 mg/mL) increased the percentage of MDSCs in culture. Both nPSG (1 and 10 mg/mL) and rPSG (10 mg/mL) increased the proportion of M-MDSC, whereas rPSG (10 mg/mL) decreased the number of PMN-MDSC.Thus, the cytokine background in CD11b+ cell cultures favored the differentiation of predominantly M-MDSC, similar to the tumor microenvironment, whereas native and recombinant PSG enhanced this effect. Thus, nPSG and rPSG are able to modulate the differentiation of MDSCs by increasing their number, mainly due to the monocytic subpopulation. This fact opens perspectives for new research on targeted manipulation of MDSCs.https://www.mimmun.ru/mimmun/article/view/2838myeloid-derived suppressor cellsimmunosuppressionpregnancy-specific β1-glycoproteincd11b+cd33+ cellsm-mdscpmn-mdsc |
| spellingShingle | V. P. Timganova K. Yu. Shardina M. S. Bochkova S. V. Uzhviyuk D. I. Usanina S. A. Zamorina Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation Медицинская иммунология myeloid-derived suppressor cells immunosuppression pregnancy-specific β1-glycoprotein cd11b+cd33+ cells m-mdsc pmn-mdsc |
| title | Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation |
| title_full | Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation |
| title_fullStr | Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation |
| title_full_unstemmed | Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation |
| title_short | Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation |
| title_sort | effect of pregnancy specific β1 glycoprotein on myeloid derived suppressor cell differentiation |
| topic | myeloid-derived suppressor cells immunosuppression pregnancy-specific β1-glycoprotein cd11b+cd33+ cells m-mdsc pmn-mdsc |
| url | https://www.mimmun.ru/mimmun/article/view/2838 |
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