<i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights
<i>Phyllanthus emblica</i> Linn. (commonly known as Amla or Indian Gooseberry) is commonly used in Ayurvedic medicine to treat respiratory infections, skin disorders, and gastrointestinal issues. The fruit contains an abundance of polyphenols, which contribute to its strong antioxidant p...
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2025-03-01
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| author | Gagan Tiwana Ian Edwin Cock Matthew James Cheesman |
| author_facet | Gagan Tiwana Ian Edwin Cock Matthew James Cheesman |
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| description | <i>Phyllanthus emblica</i> Linn. (commonly known as Amla or Indian Gooseberry) is commonly used in Ayurvedic medicine to treat respiratory infections, skin disorders, and gastrointestinal issues. The fruit contains an abundance of polyphenols, which contribute to its strong antioxidant properties. The antibacterial activity of fruit extracts derived from <i>P. emblica</i> against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Klebsiella pneumoniae</i> was determined along with the antibiotic-resistant variants extended-spectrum β-lactamase (ESBL) <i>E. coli</i>, methicillin-resistant <i>S. aureus</i> (MRSA), and ESBL <i>K. pneumoniae</i>. Disc diffusion and broth dilution assays were conducted to assess the activity of aqueous, methanolic, and ethyl acetate extracts, with large zones of inhibition of up to 15 mm on agar observed for <i>S. aureus</i> and MRSA. Minimum inhibitory concentration (MIC) values ranging from 158 to 1725 µg/mL were calculated. The aqueous and methanolic extracts of <i>P. emblica</i> were less active against <i>E. coli</i>, ESBL <i>E. coli</i>, <i>K. pneumoniae</i>, and <i>ESBL K. pneumoniae</i>, with the only noteworthy MIC (633 µg/mL) observed for the aqueous extract against <i>K. pneumoniae</i>. Interestingly, a lack of inhibition was observed on agar for any of the extracts against these bacteria. Liquid chromatography–mass spectrometry (LC-MS) analysis identified several notable flavonoids, phenolic acids, terpenoids, and tannins. Notably, <i>Artemia nauplii</i> bioassays indicated that all extracts were nontoxic. The antibacterial activity and absence of toxicity in <i>P. emblica</i> extracts suggest their potential as candidates for antibiotic development, highlighting the need for further mechanistic and phytochemical investigations. |
| format | Article |
| id | doaj-art-13b43c3a1bcb446398329b60b560313a |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-13b43c3a1bcb446398329b60b560313a2025-08-20T03:43:20ZengMDPI AGMicroorganisms2076-26072025-03-0113361110.3390/microorganisms13030611<i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity InsightsGagan Tiwana0Ian Edwin Cock1Matthew James Cheesman2School of Pharmacy and Medical Sciences, Gold Coast Campus, Griffith University, Gold Coast 4222, AustraliaSchool of Environment and Science, Nathan Campus, Griffith University, Brisbane 4111, AustraliaSchool of Pharmacy and Medical Sciences, Gold Coast Campus, Griffith University, Gold Coast 4222, Australia<i>Phyllanthus emblica</i> Linn. (commonly known as Amla or Indian Gooseberry) is commonly used in Ayurvedic medicine to treat respiratory infections, skin disorders, and gastrointestinal issues. The fruit contains an abundance of polyphenols, which contribute to its strong antioxidant properties. The antibacterial activity of fruit extracts derived from <i>P. emblica</i> against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Klebsiella pneumoniae</i> was determined along with the antibiotic-resistant variants extended-spectrum β-lactamase (ESBL) <i>E. coli</i>, methicillin-resistant <i>S. aureus</i> (MRSA), and ESBL <i>K. pneumoniae</i>. Disc diffusion and broth dilution assays were conducted to assess the activity of aqueous, methanolic, and ethyl acetate extracts, with large zones of inhibition of up to 15 mm on agar observed for <i>S. aureus</i> and MRSA. Minimum inhibitory concentration (MIC) values ranging from 158 to 1725 µg/mL were calculated. The aqueous and methanolic extracts of <i>P. emblica</i> were less active against <i>E. coli</i>, ESBL <i>E. coli</i>, <i>K. pneumoniae</i>, and <i>ESBL K. pneumoniae</i>, with the only noteworthy MIC (633 µg/mL) observed for the aqueous extract against <i>K. pneumoniae</i>. Interestingly, a lack of inhibition was observed on agar for any of the extracts against these bacteria. Liquid chromatography–mass spectrometry (LC-MS) analysis identified several notable flavonoids, phenolic acids, terpenoids, and tannins. Notably, <i>Artemia nauplii</i> bioassays indicated that all extracts were nontoxic. The antibacterial activity and absence of toxicity in <i>P. emblica</i> extracts suggest their potential as candidates for antibiotic development, highlighting the need for further mechanistic and phytochemical investigations.https://www.mdpi.com/2076-2607/13/3/611antimicrobial resistancenatural antibioticsplant-based antimicrobialscombinatorial interactionsphytochemical profilingsecondary metabolites |
| spellingShingle | Gagan Tiwana Ian Edwin Cock Matthew James Cheesman <i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights Microorganisms antimicrobial resistance natural antibiotics plant-based antimicrobials combinatorial interactions phytochemical profiling secondary metabolites |
| title | <i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights |
| title_full | <i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights |
| title_fullStr | <i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights |
| title_full_unstemmed | <i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights |
| title_short | <i>Phyllanthus emblica</i>: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights |
| title_sort | i phyllanthus emblica i phytochemistry antimicrobial potential with antibiotic enhancement and toxicity insights |
| topic | antimicrobial resistance natural antibiotics plant-based antimicrobials combinatorial interactions phytochemical profiling secondary metabolites |
| url | https://www.mdpi.com/2076-2607/13/3/611 |
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