Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation
<b>Background/Objectives</b>: Whole body vibration (WBV) is a valuable tool to mitigate physiological adaptations related to age and inactivity. Although significant benefits have been found at the musculoskeletal level, including increased bone mass and reduced muscle atrophy, the under...
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2025-01-01
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| author | Ida Cariati Roberto Bonanni Cristian Romagnoli Lucio Caprioli Giovanna D’Arcangelo Virginia Tancredi Giuseppe Annino |
| author_facet | Ida Cariati Roberto Bonanni Cristian Romagnoli Lucio Caprioli Giovanna D’Arcangelo Virginia Tancredi Giuseppe Annino |
| author_sort | Ida Cariati |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Whole body vibration (WBV) is a valuable tool to mitigate physiological adaptations related to age and inactivity. Although significant benefits have been found at the musculoskeletal level, including increased bone mass and reduced muscle atrophy, the underlying biological mechanisms remain largely unknown. Therefore, our study aimed to evaluate the effects of vibratory training on bone tissue in murine models of different age groups by investigating the structural and distribution changes in some crucial biomarkers involved in musculoskeletal homeostasis. <b>Methods</b>: Specifically, 4-, 12-, and 24-month-old mice were trained with a WBV protocol characterized by three series of 2 min and 30 s, interspersed with a recovery period of the same duration, on a 3-weekly frequency for 3 months. At the end of the training, histological and morphometric analyses were conducted, in association with immunohistochemical analysis to investigate changes in the distribution of fibronectin type III domain-containing protein 5 (FNDC5), NADPH oxidase 4 (NOX4), and sirtuin 1 (SIRT1). <b>Results</b>: Our preliminary results showed that WBV improves musculoskeletal health by preserving bone architecture and promoting up-regulation of FNDC5 and SIRT1 and down-regulation of NOX4. <b>Conclusions</b>: Our study confirms vibratory training as a viable alternative to counter musculoskeletal decline in elderly and/or sedentary subjects. Further investigations should be conducted to deepen knowledge in this field and explore the role of other molecular mediators in physiological adaptations to vibration. |
| format | Article |
| id | doaj-art-13a49d99f36c4a45aee4131c713034b6 |
| institution | OA Journals |
| issn | 2411-5142 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Journal of Functional Morphology and Kinesiology |
| spelling | doaj-art-13a49d99f36c4a45aee4131c713034b62025-08-20T02:11:05ZengMDPI AGJournal of Functional Morphology and Kinesiology2411-51422025-01-011012610.3390/jfmk10010026Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker EvaluationIda Cariati0Roberto Bonanni1Cristian Romagnoli2Lucio Caprioli3Giovanna D’Arcangelo4Virginia Tancredi5Giuseppe Annino6Department of Systems Medicine, “Tor Vergata” University of Rome, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, “Tor Vergata” University of Rome, 00133 Rome, ItalyDepartment of Human Science and Promotion of Quality of Life, San Raffaele Open University, 00166 Rome, ItalySports Engineering Laboratory, Department of Industrial Engineering, “Tor Vergata” University of Rome, 00133 Rome, ItalyDepartment of Systems Medicine, “Tor Vergata” University of Rome, 00133 Rome, ItalyDepartment of Systems Medicine, “Tor Vergata” University of Rome, 00133 Rome, ItalyDepartment of Systems Medicine, “Tor Vergata” University of Rome, 00133 Rome, Italy<b>Background/Objectives</b>: Whole body vibration (WBV) is a valuable tool to mitigate physiological adaptations related to age and inactivity. Although significant benefits have been found at the musculoskeletal level, including increased bone mass and reduced muscle atrophy, the underlying biological mechanisms remain largely unknown. Therefore, our study aimed to evaluate the effects of vibratory training on bone tissue in murine models of different age groups by investigating the structural and distribution changes in some crucial biomarkers involved in musculoskeletal homeostasis. <b>Methods</b>: Specifically, 4-, 12-, and 24-month-old mice were trained with a WBV protocol characterized by three series of 2 min and 30 s, interspersed with a recovery period of the same duration, on a 3-weekly frequency for 3 months. At the end of the training, histological and morphometric analyses were conducted, in association with immunohistochemical analysis to investigate changes in the distribution of fibronectin type III domain-containing protein 5 (FNDC5), NADPH oxidase 4 (NOX4), and sirtuin 1 (SIRT1). <b>Results</b>: Our preliminary results showed that WBV improves musculoskeletal health by preserving bone architecture and promoting up-regulation of FNDC5 and SIRT1 and down-regulation of NOX4. <b>Conclusions</b>: Our study confirms vibratory training as a viable alternative to counter musculoskeletal decline in elderly and/or sedentary subjects. Further investigations should be conducted to deepen knowledge in this field and explore the role of other molecular mediators in physiological adaptations to vibration.https://www.mdpi.com/2411-5142/10/1/26whole body vibrationbonemusculoskeletal systemphysiologyagingsedentariness |
| spellingShingle | Ida Cariati Roberto Bonanni Cristian Romagnoli Lucio Caprioli Giovanna D’Arcangelo Virginia Tancredi Giuseppe Annino Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation Journal of Functional Morphology and Kinesiology whole body vibration bone musculoskeletal system physiology aging sedentariness |
| title | Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation |
| title_full | Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation |
| title_fullStr | Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation |
| title_full_unstemmed | Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation |
| title_short | Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation |
| title_sort | bone adaptations to a whole body vibration protocol in murine models of different ages a preliminary study on structural changes and biomarker evaluation |
| topic | whole body vibration bone musculoskeletal system physiology aging sedentariness |
| url | https://www.mdpi.com/2411-5142/10/1/26 |
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