Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer Activity

A new alkaloid (orychophragvioline A) and nine known compounds were yielded from the seeds of <i>Orychophragmus violaceus</i>. Their structures were determined by various spectroscopic techniques and single-crystal X-ray diffraction. Orychophragvioline A is a rare alkaloid with an unusua...

Full description

Saved in:
Bibliographic Details
Main Authors: Ya Li, Tonghe Liu, Guangjie Pan, Yihang Li, Guoxu Ma, Yong Hou, Nailiang Zhu, Xudong Xu
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/30/8/1759
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849714075253080064
author Ya Li
Tonghe Liu
Guangjie Pan
Yihang Li
Guoxu Ma
Yong Hou
Nailiang Zhu
Xudong Xu
author_facet Ya Li
Tonghe Liu
Guangjie Pan
Yihang Li
Guoxu Ma
Yong Hou
Nailiang Zhu
Xudong Xu
author_sort Ya Li
collection DOAJ
description A new alkaloid (orychophragvioline A) and nine known compounds were yielded from the seeds of <i>Orychophragmus violaceus</i>. Their structures were determined by various spectroscopic techniques and single-crystal X-ray diffraction. Orychophragvioline A is a rare alkaloid with an unusual 1-methyl-4-phenyl-2,3-diketopiperazine skeleton connected with a guanidine group via an amide bond. The results of antitumor tests in vitro showed that it exhibited prominent cytotoxicity against Hela cells with an IC<sub>50</sub> value of 11.95 ± 1.52 μM. Further investigations suggested that it significantly inhibited cellular proliferation, migration, and invasion in a dose-dependent manner, thus inducing the apoptosis of Hela cells. These findings indicate that orychophragvioline A can be regarded as a potential natural leading compound against cervical cancer.
format Article
id doaj-art-13877b425dc2455e9bdd0beed3e9d2bd
institution DOAJ
issn 1420-3049
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Molecules
spelling doaj-art-13877b425dc2455e9bdd0beed3e9d2bd2025-08-20T03:13:48ZengMDPI AGMolecules1420-30492025-04-01308175910.3390/molecules30081759Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer ActivityYa Li0Tonghe Liu1Guangjie Pan2Yihang Li3Guoxu Ma4Yong Hou5Nailiang Zhu6Xudong Xu7Department of Gynecologic Cancer, Beijing Arion Cancer Center, Beijing 100070, ChinaKey Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, ChinaCollege of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, ChinaYunnan Key Laboratory of Southern Medicine Utilization, Yunnan Branch, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Jinghong 666100, ChinaKey Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, ChinaKey Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, ChinaKey Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, ChinaKey Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, ChinaA new alkaloid (orychophragvioline A) and nine known compounds were yielded from the seeds of <i>Orychophragmus violaceus</i>. Their structures were determined by various spectroscopic techniques and single-crystal X-ray diffraction. Orychophragvioline A is a rare alkaloid with an unusual 1-methyl-4-phenyl-2,3-diketopiperazine skeleton connected with a guanidine group via an amide bond. The results of antitumor tests in vitro showed that it exhibited prominent cytotoxicity against Hela cells with an IC<sub>50</sub> value of 11.95 ± 1.52 μM. Further investigations suggested that it significantly inhibited cellular proliferation, migration, and invasion in a dose-dependent manner, thus inducing the apoptosis of Hela cells. These findings indicate that orychophragvioline A can be regarded as a potential natural leading compound against cervical cancer.https://www.mdpi.com/1420-3049/30/8/1759orychophragvioline Anatural productanti-cervical cancernew compound
spellingShingle Ya Li
Tonghe Liu
Guangjie Pan
Yihang Li
Guoxu Ma
Yong Hou
Nailiang Zhu
Xudong Xu
Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer Activity
Molecules
orychophragvioline A
natural product
anti-cervical cancer
new compound
title Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer Activity
title_full Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer Activity
title_fullStr Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer Activity
title_full_unstemmed Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer Activity
title_short Orychophragvioline A, a Novel Alkaloid Isolated from <i>Orychophragmus violaceus</i> with Anti-Cervical Cancer Activity
title_sort orychophragvioline a a novel alkaloid isolated from i orychophragmus violaceus i with anti cervical cancer activity
topic orychophragvioline A
natural product
anti-cervical cancer
new compound
url https://www.mdpi.com/1420-3049/30/8/1759
work_keys_str_mv AT yali orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity
AT tongheliu orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity
AT guangjiepan orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity
AT yihangli orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity
AT guoxuma orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity
AT yonghou orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity
AT nailiangzhu orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity
AT xudongxu orychophragviolineaanovelalkaloidisolatedfromiorychophragmusviolaceusiwithanticervicalcanceractivity