Mitotic spindle proteomics in Chinese hamster ovary cells.
Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often resul...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0020489&type=printable |
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| _version_ | 1850215348607909888 |
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| author | Mary Kate Bonner Daniel S Poole Tao Xu Ali Sarkeshik John R Yates Ahna R Skop |
| author_facet | Mary Kate Bonner Daniel S Poole Tao Xu Ali Sarkeshik John R Yates Ahna R Skop |
| author_sort | Mary Kate Bonner |
| collection | DOAJ |
| description | Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle from Chinese Hamster Ovary (CHO) cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology (MudPIT) analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene Ontology (GO) analysis to categorize proteins into cellular component groups. We then compared our data to the previously published CHO midbody proteome and identified proteins that are unique to the CHO spindle. Our data represent the first mitotic spindle proteome in CHO cells, which augments the list of mitotic spindle components from mammalian cells. |
| format | Article |
| id | doaj-art-137a0ab58ff14ce9b83a9f7d5f0cc1df |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-137a0ab58ff14ce9b83a9f7d5f0cc1df2025-08-20T02:08:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e2048910.1371/journal.pone.0020489Mitotic spindle proteomics in Chinese hamster ovary cells.Mary Kate BonnerDaniel S PooleTao XuAli SarkeshikJohn R YatesAhna R SkopMitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle from Chinese Hamster Ovary (CHO) cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology (MudPIT) analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene Ontology (GO) analysis to categorize proteins into cellular component groups. We then compared our data to the previously published CHO midbody proteome and identified proteins that are unique to the CHO spindle. Our data represent the first mitotic spindle proteome in CHO cells, which augments the list of mitotic spindle components from mammalian cells.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0020489&type=printable |
| spellingShingle | Mary Kate Bonner Daniel S Poole Tao Xu Ali Sarkeshik John R Yates Ahna R Skop Mitotic spindle proteomics in Chinese hamster ovary cells. PLoS ONE |
| title | Mitotic spindle proteomics in Chinese hamster ovary cells. |
| title_full | Mitotic spindle proteomics in Chinese hamster ovary cells. |
| title_fullStr | Mitotic spindle proteomics in Chinese hamster ovary cells. |
| title_full_unstemmed | Mitotic spindle proteomics in Chinese hamster ovary cells. |
| title_short | Mitotic spindle proteomics in Chinese hamster ovary cells. |
| title_sort | mitotic spindle proteomics in chinese hamster ovary cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0020489&type=printable |
| work_keys_str_mv | AT marykatebonner mitoticspindleproteomicsinchinesehamsterovarycells AT danielspoole mitoticspindleproteomicsinchinesehamsterovarycells AT taoxu mitoticspindleproteomicsinchinesehamsterovarycells AT alisarkeshik mitoticspindleproteomicsinchinesehamsterovarycells AT johnryates mitoticspindleproteomicsinchinesehamsterovarycells AT ahnarskop mitoticspindleproteomicsinchinesehamsterovarycells |