Identification of MhLHC gene family under iron (Fe) deficiency stress and functional characterization of MhLHCB15 gene in Malus halliana
Iron (Fe) plays a crucial role as a micronutrient in facilitating plant growth and development. Alterations in the availability of iron can trigger a response resulting in iron deficiency, ultimately affecting both plant growth and crop yield. A total of 33 light-harvesting chlorophyll a/b-binding p...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2025-01-01
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| Series: | Fruit Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/frures-0024-0039 |
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| Summary: | Iron (Fe) plays a crucial role as a micronutrient in facilitating plant growth and development. Alterations in the availability of iron can trigger a response resulting in iron deficiency, ultimately affecting both plant growth and crop yield. A total of 33 light-harvesting chlorophyll a/b-binding protein (LHC) family members were identified based on the apple genome database. The physicochemical properties, gene structures, conserved motif compositions, evolutionary relationships, and chromosomal distributions were comprehensively analyzed. Subsequently, 15 genes were selected for quantitative reverse transcription PCR (qRT-PCR), and the results demonstrated that MhLHCB15 significantly responded to Fe deficiency stress. Meanwhile, overexpression of MhLHCB15 enhanced Fe deficiency tolerance in A. thaliana and apple calli, which evoked a variety of biochemical changes: transgenic A. thaliana displayed higher photosynthetic pigments content, photosynthetic efficiency, fluorescence parameters, and chlorophyll synthases expression compared to the wild-type (WT). Transgenic A. thaliana and apple calli had markedly greater levels of osmotic regulatory substances, enzyme activities, FCR activity, and expression of Fe uptake-related genes compared to the WT; however, the MDA content and reactive oxygen species (ROS) accumulation were less than the WT. Moreover, in this study, MhLHCB15 interaction proteins SGR1, THF1, NDUFS1, CSN5b, and LHCA3 were screened, which provides a robust theoretical basis for analyzing the construction of the regulatory network of MhLHCB15 under Fe deficiency stress. |
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| ISSN: | 2769-4615 |