Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity
Dietary exposure to the plasticiser bisphenol A (BPA), an obesogenic and endocrine disruptor from plastic and epoxy resin industries, remains prevalent despite regulatory restriction and food safety efforts. BPA can be accumulated in humans and animals, potentially exerting differential health effec...
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2025-01-01
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| author | Gracia Luque Pilar Ortiz Alfonso Torres-Sánchez Alicia Ruiz-Rodríguez Ana López-Moreno Margarita Aguilera |
| author_facet | Gracia Luque Pilar Ortiz Alfonso Torres-Sánchez Alicia Ruiz-Rodríguez Ana López-Moreno Margarita Aguilera |
| author_sort | Gracia Luque |
| collection | DOAJ |
| description | Dietary exposure to the plasticiser bisphenol A (BPA), an obesogenic and endocrine disruptor from plastic and epoxy resin industries, remains prevalent despite regulatory restriction and food safety efforts. BPA can be accumulated in humans and animals, potentially exerting differential health effects based on individual metabolic capacity. This pilot study examines the impact of direct ex vivo BPA exposure on the gut microbiota of obese and normal-weight children, using 16S rRNA amplicon sequencing and anaerobic culturing combined methods. Results showed that direct xenobiotic exposure induced modifications in microbial taxa relative abundance, community structure, and diversity. Specifically, BPA reduced the abundance of bacteria belonging to the phylum <i>Bacteroidota</i>, while taxa from the phylum <i>Actinomycetota</i> were promoted. Consistently, <i>Bacteroides</i> species were classified as sensitive to BPA, whereas bacteria belonging to the class <i>Clostridia</i> were identified as resistant to BPA in our culturomics analysis. Some of the altered bacterial abundance patterns were common for both the BPA-exposed groups and the obese non-exposed group in our pilot study. These findings were also corroborated in a larger cohort of children. Future research will be essential to evaluate these microbial taxa as potential biomarkers for biomonitoring the effect of BPA and its role as an obesogenic substance in children. |
| format | Article |
| id | doaj-art-1361bbd6b289464ab9c9a99ea3a2e156 |
| institution | OA Journals |
| issn | 2039-4705 2039-4713 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Journal of Xenobiotics |
| spelling | doaj-art-1361bbd6b289464ab9c9a99ea3a2e1562025-08-20T02:01:21ZengMDPI AGJournal of Xenobiotics2039-47052039-47132025-01-011511410.3390/jox15010014Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood ObesityGracia Luque0Pilar Ortiz1Alfonso Torres-Sánchez2Alicia Ruiz-Rodríguez3Ana López-Moreno4Margarita Aguilera5Human Microbiota Laboratory, Institute of Nutrition and Food Technology “José Mataix” (INYTA), Centre of Biomedical Research, University of Granada, 18016 Granada, SpainHuman Microbiota Laboratory, Institute of Nutrition and Food Technology “José Mataix” (INYTA), Centre of Biomedical Research, University of Granada, 18016 Granada, SpainHuman Microbiota Laboratory, Institute of Nutrition and Food Technology “José Mataix” (INYTA), Centre of Biomedical Research, University of Granada, 18016 Granada, SpainHuman Microbiota Laboratory, Institute of Nutrition and Food Technology “José Mataix” (INYTA), Centre of Biomedical Research, University of Granada, 18016 Granada, SpainHuman Microbiota Laboratory, Institute of Nutrition and Food Technology “José Mataix” (INYTA), Centre of Biomedical Research, University of Granada, 18016 Granada, SpainHuman Microbiota Laboratory, Institute of Nutrition and Food Technology “José Mataix” (INYTA), Centre of Biomedical Research, University of Granada, 18016 Granada, SpainDietary exposure to the plasticiser bisphenol A (BPA), an obesogenic and endocrine disruptor from plastic and epoxy resin industries, remains prevalent despite regulatory restriction and food safety efforts. BPA can be accumulated in humans and animals, potentially exerting differential health effects based on individual metabolic capacity. This pilot study examines the impact of direct ex vivo BPA exposure on the gut microbiota of obese and normal-weight children, using 16S rRNA amplicon sequencing and anaerobic culturing combined methods. Results showed that direct xenobiotic exposure induced modifications in microbial taxa relative abundance, community structure, and diversity. Specifically, BPA reduced the abundance of bacteria belonging to the phylum <i>Bacteroidota</i>, while taxa from the phylum <i>Actinomycetota</i> were promoted. Consistently, <i>Bacteroides</i> species were classified as sensitive to BPA, whereas bacteria belonging to the class <i>Clostridia</i> were identified as resistant to BPA in our culturomics analysis. Some of the altered bacterial abundance patterns were common for both the BPA-exposed groups and the obese non-exposed group in our pilot study. These findings were also corroborated in a larger cohort of children. Future research will be essential to evaluate these microbial taxa as potential biomarkers for biomonitoring the effect of BPA and its role as an obesogenic substance in children.https://www.mdpi.com/2039-4713/15/1/14ex vivo BPA exposuremicrobiota taxadysbiosisobesity |
| spellingShingle | Gracia Luque Pilar Ortiz Alfonso Torres-Sánchez Alicia Ruiz-Rodríguez Ana López-Moreno Margarita Aguilera Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity Journal of Xenobiotics ex vivo BPA exposure microbiota taxa dysbiosis obesity |
| title | Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity |
| title_full | Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity |
| title_fullStr | Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity |
| title_full_unstemmed | Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity |
| title_short | Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity |
| title_sort | impact of ex vivo bisphenol a exposure on gut microbiota dysbiosis and its association with childhood obesity |
| topic | ex vivo BPA exposure microbiota taxa dysbiosis obesity |
| url | https://www.mdpi.com/2039-4713/15/1/14 |
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