Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration
Abstract Effective treatment of skin wounds remains a clinical challenge owing to factors such as microbial infections, impaired fibroblast activity, disrupted angiogenesis, and collagen remodeling. In this study, we developed and evaluated novel curcumin–cyclodextrin hybrid nanoparticles (Cur/CD-HN...
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2025-06-01
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| Online Access: | https://doi.org/10.1186/s12896-025-00989-z |
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| author | Heidi M. Abdel-Mageed Nermeen Z. AbuelEzz Ahmed A. Ali Amira Emad Abdelaziz Dina Nada Sahar M. Abdelraouf Shahinaze A. Fouad Abeer Bishr Rasha A. Radwan |
| author_facet | Heidi M. Abdel-Mageed Nermeen Z. AbuelEzz Ahmed A. Ali Amira Emad Abdelaziz Dina Nada Sahar M. Abdelraouf Shahinaze A. Fouad Abeer Bishr Rasha A. Radwan |
| author_sort | Heidi M. Abdel-Mageed |
| collection | DOAJ |
| description | Abstract Effective treatment of skin wounds remains a clinical challenge owing to factors such as microbial infections, impaired fibroblast activity, disrupted angiogenesis, and collagen remodeling. In this study, we developed and evaluated novel curcumin–cyclodextrin hybrid nanoparticles (Cur/CD-HNPs) as a multifunctional platform for enhanced wound healing. Nanoparticles (NPs) were prepared via nanoprecipitation. Physicochemical and structural properties were systematically characterized by determining the encapsulation efficiency (EE), particle size, zeta potential, X-XRD, FTIR, SEM, in vitro release, and stability studies. The optimized Cur/CD-HNPs demonstrated a uniform particle size of 150.5 ± 2.8 nm, a surface charge of − 18.5 ± 0.59 mV, a PDI of 0.20 ± 0.03, and a high EE (90.2 ± 2.35%). Cur/CD-HNPs exhibited potent anti-inflammatory effects (97.93 ± 1.24% inhibition of protein denaturation), full antioxidant activity (100% ABTS radical scavenging, IC50 = 12.85 µg/mL), and broad-spectrum antibacterial efficacy. Cur/CD-HNPs exhibited a sustained biphasic release profile, with ~ 82% of Cur released over 24 h, supporting sustained delivery for wound healing applications. In vitro scratch assays revealed enhanced fibroblast proliferation and migration. For in vivo evaluation, the nanoparticles were incorporated into a hydrogel base and applied topically in a rat burn wound model, resulting in significantly accelerated wound closure (P < 0.05). Histopathological examination revealed improved epithelialization, collagen deposition, and tissue regeneration compared with the control groups. Our findings presented Cur/CD-HNPs as a promising therapeutic approach, offering Cur enhanced bioactivity, stability, and regenerative potential. This formulation addresses the key limitations of curcumin and presents a multifunctional and strong translational platform for clinical wound care. Graphical Abstract |
| format | Article |
| id | doaj-art-13590412e2f743cdb58b4e01f35a80e8 |
| institution | DOAJ |
| issn | 1472-6750 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
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| series | BMC Biotechnology |
| spelling | doaj-art-13590412e2f743cdb58b4e01f35a80e82025-08-20T03:22:50ZengBMCBMC Biotechnology1472-67502025-06-0125112310.1186/s12896-025-00989-zNewly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regenerationHeidi M. Abdel-Mageed0Nermeen Z. AbuelEzz1Ahmed A. Ali2Amira Emad Abdelaziz3Dina Nada4Sahar M. Abdelraouf5Shahinaze A. Fouad6Abeer Bishr7Rasha A. Radwan8Molecular Biology Department, National Research CentreBiochemistry Department, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and TechnologyMolecular Biology Department, National Research CentrePharmacology Department, Clinical and Biological Sciences Departments, College of Pharmacy, Arab Academy for Science and Technology and Maritime TransportPharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE)Department of Biochemistry, Faculty of Pharmacy, Misr International University (MIU)Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Ahram Canadian UniversityPharmacology and Toxicology Department, Faculty of Pharmacy, Ahram Canadian UniversityBiochemistry Department, Faculty of Biotechnology, German International UniversityAbstract Effective treatment of skin wounds remains a clinical challenge owing to factors such as microbial infections, impaired fibroblast activity, disrupted angiogenesis, and collagen remodeling. In this study, we developed and evaluated novel curcumin–cyclodextrin hybrid nanoparticles (Cur/CD-HNPs) as a multifunctional platform for enhanced wound healing. Nanoparticles (NPs) were prepared via nanoprecipitation. Physicochemical and structural properties were systematically characterized by determining the encapsulation efficiency (EE), particle size, zeta potential, X-XRD, FTIR, SEM, in vitro release, and stability studies. The optimized Cur/CD-HNPs demonstrated a uniform particle size of 150.5 ± 2.8 nm, a surface charge of − 18.5 ± 0.59 mV, a PDI of 0.20 ± 0.03, and a high EE (90.2 ± 2.35%). Cur/CD-HNPs exhibited potent anti-inflammatory effects (97.93 ± 1.24% inhibition of protein denaturation), full antioxidant activity (100% ABTS radical scavenging, IC50 = 12.85 µg/mL), and broad-spectrum antibacterial efficacy. Cur/CD-HNPs exhibited a sustained biphasic release profile, with ~ 82% of Cur released over 24 h, supporting sustained delivery for wound healing applications. In vitro scratch assays revealed enhanced fibroblast proliferation and migration. For in vivo evaluation, the nanoparticles were incorporated into a hydrogel base and applied topically in a rat burn wound model, resulting in significantly accelerated wound closure (P < 0.05). Histopathological examination revealed improved epithelialization, collagen deposition, and tissue regeneration compared with the control groups. Our findings presented Cur/CD-HNPs as a promising therapeutic approach, offering Cur enhanced bioactivity, stability, and regenerative potential. This formulation addresses the key limitations of curcumin and presents a multifunctional and strong translational platform for clinical wound care. Graphical Abstracthttps://doi.org/10.1186/s12896-025-00989-zCurcuminHydrogelsWound healingAntioxidant anti-inflammatoryHybrid nanoparticlesCyclodextrin |
| spellingShingle | Heidi M. Abdel-Mageed Nermeen Z. AbuelEzz Ahmed A. Ali Amira Emad Abdelaziz Dina Nada Sahar M. Abdelraouf Shahinaze A. Fouad Abeer Bishr Rasha A. Radwan Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration BMC Biotechnology Curcumin Hydrogels Wound healing Antioxidant anti-inflammatory Hybrid nanoparticles Cyclodextrin |
| title | Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration |
| title_full | Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration |
| title_fullStr | Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration |
| title_full_unstemmed | Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration |
| title_short | Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration |
| title_sort | newly designed curcumin loaded hybrid nanoparticles a multifunctional strategy for combating oxidative stress inflammation and infections to accelerate wound healing and tissue regeneration |
| topic | Curcumin Hydrogels Wound healing Antioxidant anti-inflammatory Hybrid nanoparticles Cyclodextrin |
| url | https://doi.org/10.1186/s12896-025-00989-z |
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