Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis
This study investigated whether the administration of resolvin D1 to rats with endotoxininduced uveitis (EIU) ameliorates the immuno-inflammatory profile of the eye. 24 h after the administration of 200 μg LPS into the footpad of Sprague-Dawley rats, severe changes of the structure of the eye occurr...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/318621 |
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| author | Rossi Settimio Di Filippo Clara Ferraraccio Franca Simonelli Francesca D'Amico Michele |
| author_facet | Rossi Settimio Di Filippo Clara Ferraraccio Franca Simonelli Francesca D'Amico Michele |
| author_sort | Rossi Settimio |
| collection | DOAJ |
| description | This study investigated whether the administration of resolvin D1 to rats with endotoxininduced uveitis (EIU) ameliorates the immuno-inflammatory profile of the eye. 24 h after the administration of 200 μg LPS into the footpad of Sprague-Dawley rats, severe changes of the structure of the eye occurred concomitantly with a severe inflammatory and immune response. These latter included strong infiltration of PMN leukocytes CD11b+ T-lymphocytes CD4+ and CD8+ within the eye and a significant release of the cytokines/chemokines TNF-alpha, CXCL8, and RANTES too. Bolus of resolvin D1 (RvD1; 10–100–1000 ng/kg in 200 μL of sterile saline via the tail vein) significantly and dose-dependently (i) reduced the development of the ocular derangement caused by LPS; (ii) reduced the clinical score attributed to EIU; (iii) reduced the protein concentration and myeloperoxidase activity (MPO) in aqueous humor (AqH); and (iv) reduced neutrophils, T-lymphocytes, and cytokines within the eye. |
| format | Article |
| id | doaj-art-13459bda774e4d009a42fa84f255d680 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-13459bda774e4d009a42fa84f255d6802025-02-03T05:51:32ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/318621318621Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following UveitisRossi Settimio0Di Filippo Clara1Ferraraccio Franca2Simonelli Francesca3D'Amico Michele4Department of Ophthalmology, Second University of Naples, 80100 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, Second University of Naples, Via Costantinopoli 16, 80138 Naples, ItalyDepartment of Clinical, Public and Preventive Medicine, Second University of Naples, 80138 Naples, ItalyDepartment of Ophthalmology, Second University of Naples, 80100 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, Second University of Naples, Via Costantinopoli 16, 80138 Naples, ItalyThis study investigated whether the administration of resolvin D1 to rats with endotoxininduced uveitis (EIU) ameliorates the immuno-inflammatory profile of the eye. 24 h after the administration of 200 μg LPS into the footpad of Sprague-Dawley rats, severe changes of the structure of the eye occurred concomitantly with a severe inflammatory and immune response. These latter included strong infiltration of PMN leukocytes CD11b+ T-lymphocytes CD4+ and CD8+ within the eye and a significant release of the cytokines/chemokines TNF-alpha, CXCL8, and RANTES too. Bolus of resolvin D1 (RvD1; 10–100–1000 ng/kg in 200 μL of sterile saline via the tail vein) significantly and dose-dependently (i) reduced the development of the ocular derangement caused by LPS; (ii) reduced the clinical score attributed to EIU; (iii) reduced the protein concentration and myeloperoxidase activity (MPO) in aqueous humor (AqH); and (iv) reduced neutrophils, T-lymphocytes, and cytokines within the eye.http://dx.doi.org/10.1155/2012/318621 |
| spellingShingle | Rossi Settimio Di Filippo Clara Ferraraccio Franca Simonelli Francesca D'Amico Michele Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis Mediators of Inflammation |
| title | Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis |
| title_full | Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis |
| title_fullStr | Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis |
| title_full_unstemmed | Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis |
| title_short | Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis |
| title_sort | resolvin d1 reduces the immunoinflammatory response of the rat eye following uveitis |
| url | http://dx.doi.org/10.1155/2012/318621 |
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