Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation

The occurrence of SOX2-specific autoantibodies seems to be associated with an improved prognosis in patients with monoclonal gammopathy of undetermined significance (MGUS). However, it is unclear if SOX2-specific antibodies also develop in established multiple myeloma (MM). Screening 1094 peripheral...

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Main Authors: Sebastian Kobold, Sinje Tams, Tim Luetkens, Yanran Cao, Orhan Sezer, Britta Marlen Bartels, Henrike Reinhard, Julia Templin, Katrin Bartels, York Hildebrandt, Nesrine Lajmi, Andreas Marx, Friedrich Haag, Carsten Bokemeyer, Nicolaus Kröger, Djordje Atanackovic
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Clinical and Developmental Immunology
Online Access:http://dx.doi.org/10.1155/2011/302145
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author Sebastian Kobold
Sinje Tams
Tim Luetkens
Yanran Cao
Orhan Sezer
Britta Marlen Bartels
Henrike Reinhard
Julia Templin
Katrin Bartels
York Hildebrandt
Nesrine Lajmi
Andreas Marx
Friedrich Haag
Carsten Bokemeyer
Nicolaus Kröger
Djordje Atanackovic
author_facet Sebastian Kobold
Sinje Tams
Tim Luetkens
Yanran Cao
Orhan Sezer
Britta Marlen Bartels
Henrike Reinhard
Julia Templin
Katrin Bartels
York Hildebrandt
Nesrine Lajmi
Andreas Marx
Friedrich Haag
Carsten Bokemeyer
Nicolaus Kröger
Djordje Atanackovic
author_sort Sebastian Kobold
collection DOAJ
description The occurrence of SOX2-specific autoantibodies seems to be associated with an improved prognosis in patients with monoclonal gammopathy of undetermined significance (MGUS). However, it is unclear if SOX2-specific antibodies also develop in established multiple myeloma (MM). Screening 1094 peripheral blood (PB) sera from 196 MM patients and 100 PB sera from healthy donors, we detected SOX2-specific autoantibodies in 7.7% and 2.0% of patients and donors, respectively. We identified SOX2211–230 as an immunodominant antibody-epitope within the full protein sequence. SOX2 antigen was expressed in most healthy tissues and its expression did not correlate with the number of BM-resident plasma cells. Accordingly, anti-SOX2 immunity was not related to SOX2 expression levels or tumor burden in the patients’ BM. The only clinical factor predicting the development of anti-SOX2 immunity was application of allogeneic stem cell transplantation (alloSCT). Anti-SOX2 antibodies occurred more frequently in patients who had received alloSCT (n=74). Moreover, most SOX2-seropositive patients had only developed antibodies after alloSCT. This finding indicates that alloSCT is able to break tolerance towards this commonly expressed antigen. The questions whether SOX2-specific autoantibodies merely represent an epiphenomenon, are related to graft-versus-host effects or participate in the immune control of myeloma needs to be answered in prospective studies.
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spelling doaj-art-1340e985962c441bb5cef8f5304ff6f52025-08-20T03:54:33ZengWileyClinical and Developmental Immunology1740-25221740-25302011-01-01201110.1155/2011/302145302145Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell TransplantationSebastian Kobold0Sinje Tams1Tim Luetkens2Yanran Cao3Orhan Sezer4Britta Marlen Bartels5Henrike Reinhard6Julia Templin7Katrin Bartels8York Hildebrandt9Nesrine Lajmi10Andreas Marx11Friedrich Haag12Carsten Bokemeyer13Nicolaus Kröger14Djordje Atanackovic15Department of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyInstitute for Pathology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyInstitute for Immunology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyDepartment of Internal Medicine II, Oncology/Hematology/Bone Marrow Transplantation with the Section Pneumology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyThe occurrence of SOX2-specific autoantibodies seems to be associated with an improved prognosis in patients with monoclonal gammopathy of undetermined significance (MGUS). However, it is unclear if SOX2-specific antibodies also develop in established multiple myeloma (MM). Screening 1094 peripheral blood (PB) sera from 196 MM patients and 100 PB sera from healthy donors, we detected SOX2-specific autoantibodies in 7.7% and 2.0% of patients and donors, respectively. We identified SOX2211–230 as an immunodominant antibody-epitope within the full protein sequence. SOX2 antigen was expressed in most healthy tissues and its expression did not correlate with the number of BM-resident plasma cells. Accordingly, anti-SOX2 immunity was not related to SOX2 expression levels or tumor burden in the patients’ BM. The only clinical factor predicting the development of anti-SOX2 immunity was application of allogeneic stem cell transplantation (alloSCT). Anti-SOX2 antibodies occurred more frequently in patients who had received alloSCT (n=74). Moreover, most SOX2-seropositive patients had only developed antibodies after alloSCT. This finding indicates that alloSCT is able to break tolerance towards this commonly expressed antigen. The questions whether SOX2-specific autoantibodies merely represent an epiphenomenon, are related to graft-versus-host effects or participate in the immune control of myeloma needs to be answered in prospective studies.http://dx.doi.org/10.1155/2011/302145
spellingShingle Sebastian Kobold
Sinje Tams
Tim Luetkens
Yanran Cao
Orhan Sezer
Britta Marlen Bartels
Henrike Reinhard
Julia Templin
Katrin Bartels
York Hildebrandt
Nesrine Lajmi
Andreas Marx
Friedrich Haag
Carsten Bokemeyer
Nicolaus Kröger
Djordje Atanackovic
Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation
Clinical and Developmental Immunology
title Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation
title_full Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation
title_fullStr Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation
title_full_unstemmed Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation
title_short Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation
title_sort patients with multiple myeloma develop sox2 specific autoantibodies after allogeneic stem cell transplantation
url http://dx.doi.org/10.1155/2011/302145
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