Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus

Abstract The highly pathogenic avian influenza A(H5N1) virus threatens animal and human health globally. Innovative strategies are crucial for mitigating risks associated with airborne transmission and preventing outbreaks. In this study, we sought to investigate the efficacy of microwave inactivati...

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Main Authors: Pietro Bia, Margherita Losardo, Antonio Manna, Silvio Brusaferro, Gaetano P. Privitera, Alberto Sangiovanni Vincentelli
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-85376-6
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author Pietro Bia
Margherita Losardo
Antonio Manna
Silvio Brusaferro
Gaetano P. Privitera
Alberto Sangiovanni Vincentelli
author_facet Pietro Bia
Margherita Losardo
Antonio Manna
Silvio Brusaferro
Gaetano P. Privitera
Alberto Sangiovanni Vincentelli
author_sort Pietro Bia
collection DOAJ
description Abstract The highly pathogenic avian influenza A(H5N1) virus threatens animal and human health globally. Innovative strategies are crucial for mitigating risks associated with airborne transmission and preventing outbreaks. In this study, we sought to investigate the efficacy of microwave inactivation against aerosolized A(H5N1) virus by identifying the optimal frequency band for a 10-min exposure and evaluating the impact of varying exposure times on virus inactivation. A(H5N1) was aerosolized and exposed to various microwave frequencies ranging from 8 to 16 GHz for a duration of 10 min. Viral titers were quantified using TCID50, and inactivation was assessed by comparing irradiated samples to controls. The 11–13 GHz band yielded the highest inactivation, with an average 89% mean reduction in A(H5N1) titer, particularly within the 11–12 GHz range, which exhibited peak efficacy. Based on the overall results, the optimal frequency band (8–12 GHz) was further tested with exposure durations of 1, 3, and 5 min. Inactivation was time-dependent, with a 5-minute exposure resulting in a 94% mean reduction, compared to 58% and 48% for 3- and 1-minute exposures, respectively. We conclude that optimized microwave emitters in high-risk environments like poultry farms and veterinary clinics could offer a novel, non-chemical approach to mitigating avian influenza spread and outbreaks.
format Article
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institution Kabale University
issn 2045-2322
language English
publishDate 2025-01-01
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spelling doaj-art-133af46c59744ce198092d2f5af22cce2025-01-19T12:23:01ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-025-85376-6Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virusPietro Bia0Margherita Losardo1Antonio Manna2Silvio Brusaferro3Gaetano P. Privitera4Alberto Sangiovanni Vincentelli5Elettronica S.p.AElettronica S.p.AElettronica S.p.ADepartment of Medicine, University of UdineDepartment of Translational Research and New Technologies in Medicine and Surgery, University of PisaThe Edgar L. and Harold H. Buttner Chair of Electrical Engineering and Computer Sciences, University of CaliforniaAbstract The highly pathogenic avian influenza A(H5N1) virus threatens animal and human health globally. Innovative strategies are crucial for mitigating risks associated with airborne transmission and preventing outbreaks. In this study, we sought to investigate the efficacy of microwave inactivation against aerosolized A(H5N1) virus by identifying the optimal frequency band for a 10-min exposure and evaluating the impact of varying exposure times on virus inactivation. A(H5N1) was aerosolized and exposed to various microwave frequencies ranging from 8 to 16 GHz for a duration of 10 min. Viral titers were quantified using TCID50, and inactivation was assessed by comparing irradiated samples to controls. The 11–13 GHz band yielded the highest inactivation, with an average 89% mean reduction in A(H5N1) titer, particularly within the 11–12 GHz range, which exhibited peak efficacy. Based on the overall results, the optimal frequency band (8–12 GHz) was further tested with exposure durations of 1, 3, and 5 min. Inactivation was time-dependent, with a 5-minute exposure resulting in a 94% mean reduction, compared to 58% and 48% for 3- and 1-minute exposures, respectively. We conclude that optimized microwave emitters in high-risk environments like poultry farms and veterinary clinics could offer a novel, non-chemical approach to mitigating avian influenza spread and outbreaks.https://doi.org/10.1038/s41598-025-85376-6Avian influenzaA(H5N1) virusRadiated microwavesFrequency bandsExposure time
spellingShingle Pietro Bia
Margherita Losardo
Antonio Manna
Silvio Brusaferro
Gaetano P. Privitera
Alberto Sangiovanni Vincentelli
Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus
Scientific Reports
Avian influenza
A(H5N1) virus
Radiated microwaves
Frequency bands
Exposure time
title Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus
title_full Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus
title_fullStr Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus
title_full_unstemmed Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus
title_short Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus
title_sort selected microwave irradiation effectively inactivates airborne avian influenza a h5n1 virus
topic Avian influenza
A(H5N1) virus
Radiated microwaves
Frequency bands
Exposure time
url https://doi.org/10.1038/s41598-025-85376-6
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