<i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting Effect
Pepper Phytophthora blight caused by <i>Phytophthora capsici</i> results in substantial losses in global pepper cultivation. The use of biocontrol agents with the dual functions of disease suppression and crop growth promotion is a green and sustainable way of managing this pathogen. In...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/2/105 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850081266526846976 |
|---|---|
| author | Jien Zhou Junfeng Liang Xueyan Zhang Feng Wang Zheng Qu Tongguo Gao Yanpo Yao Yanli Luo |
| author_facet | Jien Zhou Junfeng Liang Xueyan Zhang Feng Wang Zheng Qu Tongguo Gao Yanpo Yao Yanli Luo |
| author_sort | Jien Zhou |
| collection | DOAJ |
| description | Pepper Phytophthora blight caused by <i>Phytophthora capsici</i> results in substantial losses in global pepper cultivation. The use of biocontrol agents with the dual functions of disease suppression and crop growth promotion is a green and sustainable way of managing this pathogen. In this study, six biocontrol strains of <i>Trichoderma</i> with high antagonistic activity against <i>P. capsici</i> were isolated and screened from the rhizosphere soil of healthy peppers undergoing long-term continuous cultivation. Morphological identification and molecular biological identification revealed that strains 2213 and 2221 were <i>T. harzianum</i>, strains 5111, 6311, and 6321 were <i>T. brevicompactum</i>, and strain 7111 was <i>T. virens</i>. The results showed that <i>T. brevicompactum</i> 6311 had the greatest inhibitory effect against <i>P. capsici</i>. The inhibition rate of 6311 on the mycelial growth of <i>P. capsici</i> was 82.22% in a double-culture test, whereas it reached 100% in a fermentation liquid culture test. Meanwhile, the pepper fruit tests showed that 6311 was 29% effective against <i>P. capsici</i> on pepper, and a potting test demonstrated that the preventive and controlling effect of 6311 on pepper epidemics triggered by <i>P. capsici</i> was 55.56%. The growth-promoting effect, germination potential, germination rate, radicle-embryonic axis length, germination index, and fresh weight of peppers cultured in the 6311 fermentation broth were significantly increased compared with the results for the control group. Scanning electron microscopy revealed that 6311 achieved the parasitism of <i>P. capsici</i>, producing siderophores and the growth hormone indoleacetic acid (IAA) to achieve disease-suppressive and growth-promoting functions. Transcriptomic results indicated that genes encoding proteins involved in plant disease resistance, namely flavanone 3-hydroxylase (F3H) and growth transcription factor (AUX22), were generally upregulated after the application of 6311. This study demonstrated that 6311 exhibits significant bioprotective and growth-promoting functions. |
| format | Article |
| id | doaj-art-1339f02d24b94e5da4ee8a3acd0d20bb |
| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-1339f02d24b94e5da4ee8a3acd0d20bb2025-08-20T02:44:46ZengMDPI AGJournal of Fungi2309-608X2025-01-0111210510.3390/jof11020105<i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting EffectJien Zhou0Junfeng Liang1Xueyan Zhang2Feng Wang3Zheng Qu4Tongguo Gao5Yanpo Yao6Yanli Luo7College of Resources and Environment, Xinjiang Agricultural University, Urumqi 830052, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071002, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaCollege of Resources and Environment, Xinjiang Agricultural University, Urumqi 830052, ChinaPepper Phytophthora blight caused by <i>Phytophthora capsici</i> results in substantial losses in global pepper cultivation. The use of biocontrol agents with the dual functions of disease suppression and crop growth promotion is a green and sustainable way of managing this pathogen. In this study, six biocontrol strains of <i>Trichoderma</i> with high antagonistic activity against <i>P. capsici</i> were isolated and screened from the rhizosphere soil of healthy peppers undergoing long-term continuous cultivation. Morphological identification and molecular biological identification revealed that strains 2213 and 2221 were <i>T. harzianum</i>, strains 5111, 6311, and 6321 were <i>T. brevicompactum</i>, and strain 7111 was <i>T. virens</i>. The results showed that <i>T. brevicompactum</i> 6311 had the greatest inhibitory effect against <i>P. capsici</i>. The inhibition rate of 6311 on the mycelial growth of <i>P. capsici</i> was 82.22% in a double-culture test, whereas it reached 100% in a fermentation liquid culture test. Meanwhile, the pepper fruit tests showed that 6311 was 29% effective against <i>P. capsici</i> on pepper, and a potting test demonstrated that the preventive and controlling effect of 6311 on pepper epidemics triggered by <i>P. capsici</i> was 55.56%. The growth-promoting effect, germination potential, germination rate, radicle-embryonic axis length, germination index, and fresh weight of peppers cultured in the 6311 fermentation broth were significantly increased compared with the results for the control group. Scanning electron microscopy revealed that 6311 achieved the parasitism of <i>P. capsici</i>, producing siderophores and the growth hormone indoleacetic acid (IAA) to achieve disease-suppressive and growth-promoting functions. Transcriptomic results indicated that genes encoding proteins involved in plant disease resistance, namely flavanone 3-hydroxylase (F3H) and growth transcription factor (AUX22), were generally upregulated after the application of 6311. This study demonstrated that 6311 exhibits significant bioprotective and growth-promoting functions.https://www.mdpi.com/2309-608X/11/2/105<i>T. brevicompactum</i>biocontrol strainssoil-borne pathogenspepper Phytophthora blightdisease inhibitiongrowth promotion |
| spellingShingle | Jien Zhou Junfeng Liang Xueyan Zhang Feng Wang Zheng Qu Tongguo Gao Yanpo Yao Yanli Luo <i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting Effect Journal of Fungi <i>T. brevicompactum</i> biocontrol strains soil-borne pathogens pepper Phytophthora blight disease inhibition growth promotion |
| title | <i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting Effect |
| title_full | <i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting Effect |
| title_fullStr | <i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting Effect |
| title_full_unstemmed | <i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting Effect |
| title_short | <i>Trichoderma brevicompactum</i> 6311: Prevention and Control of <i>Phytophthora capsici</i> and Its Growth-Promoting Effect |
| title_sort | i trichoderma brevicompactum i 6311 prevention and control of i phytophthora capsici i and its growth promoting effect |
| topic | <i>T. brevicompactum</i> biocontrol strains soil-borne pathogens pepper Phytophthora blight disease inhibition growth promotion |
| url | https://www.mdpi.com/2309-608X/11/2/105 |
| work_keys_str_mv | AT jienzhou itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect AT junfengliang itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect AT xueyanzhang itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect AT fengwang itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect AT zhengqu itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect AT tongguogao itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect AT yanpoyao itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect AT yanliluo itrichodermabrevicompactumi6311preventionandcontrolofiphytophthoracapsiciianditsgrowthpromotingeffect |