Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia

Efforts to optimize ex vitro transfer techniques of un-rooted in vitro shoots of Cinchona spp. (Rubiaceae) a native tree of the andean forests, are crucial for promoting sustainable cultivation practices of this medicinal plant. Renowned for its historical significance and effectiveness in treating...

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Main Authors: Juan David Saavedra Correa, Silvia Lizette Bustamante Rodriguez, Robert Theiler
Format: Article
Language:Spanish
Published: Universidad Nacional de Colombia 2025-06-01
Series:Revista Colombiana de Biotecnología
Subjects:
Online Access:https://revistas.unal.edu.co/index.php/biotecnologia/article/view/118377
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author Juan David Saavedra Correa
Silvia Lizette Bustamante Rodriguez
Robert Theiler
author_facet Juan David Saavedra Correa
Silvia Lizette Bustamante Rodriguez
Robert Theiler
author_sort Juan David Saavedra Correa
collection DOAJ
description Efforts to optimize ex vitro transfer techniques of un-rooted in vitro shoots of Cinchona spp. (Rubiaceae) a native tree of the andean forests, are crucial for promoting sustainable cultivation practices of this medicinal plant. Renowned for its historical significance and effectiveness in treating malaria and other ailments, the tree holds a prominent position in botanical research, food industry, and pharmaceutical applications due to its production of alkaloids such as quinine and quinidine. This study aimed to improve the acclimatization process of un-rooted in vitro shoots of Cinchona, facilitating their transplantation to field conditions for establishing a plantation in Colombia. The results revealed that compared to commercial available substrates (pH higher than 5.5), the utilization of peat moss substrate (pH lower than 4 and electric conductivity lower than 100 μS / cm-1) led to significantly higher survival rates (>87%) and improved growth outcomes. This underscores the efficacy of peat moss in facilitating the acclimatization process of ex vitro plants over a 12-week period, ensuring robust development and survival of the plants, which guarantees vigorous specimens for field plantation.
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series Revista Colombiana de Biotecnología
spelling doaj-art-132b77781d0142c7bc7e79d6ac04471e2025-08-20T02:02:57ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582025-06-0127110.15446/rev.colomb.biote.v27n1.118377Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in ColombiaJuan David Saavedra Correahttps://orcid.org/0000-0003-1527-0428Silvia Lizette Bustamante Rodriguez0https://orcid.org/0000-0002-2595-9758Robert Theiler1https://orcid.org/0009-0003-3705-6542Instituto de Biotecnologia Universidad Nacional de ColombiaFormerly Horticultural Department, Agroscope Changins-Wädenswil Efforts to optimize ex vitro transfer techniques of un-rooted in vitro shoots of Cinchona spp. (Rubiaceae) a native tree of the andean forests, are crucial for promoting sustainable cultivation practices of this medicinal plant. Renowned for its historical significance and effectiveness in treating malaria and other ailments, the tree holds a prominent position in botanical research, food industry, and pharmaceutical applications due to its production of alkaloids such as quinine and quinidine. This study aimed to improve the acclimatization process of un-rooted in vitro shoots of Cinchona, facilitating their transplantation to field conditions for establishing a plantation in Colombia. The results revealed that compared to commercial available substrates (pH higher than 5.5), the utilization of peat moss substrate (pH lower than 4 and electric conductivity lower than 100 μS / cm-1) led to significantly higher survival rates (>87%) and improved growth outcomes. This underscores the efficacy of peat moss in facilitating the acclimatization process of ex vitro plants over a 12-week period, ensuring robust development and survival of the plants, which guarantees vigorous specimens for field plantation. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/118377CinchonaEx Vitro transferAcclimatizationUnrooted shoots
spellingShingle Juan David Saavedra Correa
Silvia Lizette Bustamante Rodriguez
Robert Theiler
Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia
Revista Colombiana de Biotecnología
Cinchona
Ex Vitro transfer
Acclimatization
Unrooted shoots
title Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia
title_full Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia
title_fullStr Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia
title_full_unstemmed Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia
title_short Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia
title_sort optimizing ex vitro acclimatization of cinchona spp towards the establishment of an industrial crop in colombia
topic Cinchona
Ex Vitro transfer
Acclimatization
Unrooted shoots
url https://revistas.unal.edu.co/index.php/biotecnologia/article/view/118377
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