Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DC
Aromatic plants are rich sources of essential oils (EOs), recognized for their therapeutic properties due to their diversity of phytochemicals. This study investigated the anxiolytic and antidepressant effects of <i>Myrcia sylvatica</i> essential oil (MsEO) through inhalation in an anima...
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MDPI AG
2025-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/15/1/110 |
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| author | Antônio Quaresma da Silva Júnior Mariana Maciel Garcia Wanderson da Silva Farias Deise Juliane dos Anjos de Sousa Adenilson de Sousa Barroso Pablo Luis Baia Figueiredo Gabriela B. dos Santos Ricardo Bezerra de Oliveira Rosa Helena Veras Mourão |
| author_facet | Antônio Quaresma da Silva Júnior Mariana Maciel Garcia Wanderson da Silva Farias Deise Juliane dos Anjos de Sousa Adenilson de Sousa Barroso Pablo Luis Baia Figueiredo Gabriela B. dos Santos Ricardo Bezerra de Oliveira Rosa Helena Veras Mourão |
| author_sort | Antônio Quaresma da Silva Júnior |
| collection | DOAJ |
| description | Aromatic plants are rich sources of essential oils (EOs), recognized for their therapeutic properties due to their diversity of phytochemicals. This study investigated the anxiolytic and antidepressant effects of <i>Myrcia sylvatica</i> essential oil (MsEO) through inhalation in an animal model and its in vitro anticholinesterase (AChE) activity. The EO was obtained by hydrodistillation, and its volatile constituents were analyzed by GC-MS. Swiss mice were exposed to doses of 0.1%, 1%, and 2% of the EO via an inhalation apparatus. The anxiolytic activity was assessed using the elevated plus maze and light–dark box tests, while antidepressant activity was evaluated using the tail suspension and forced swimming tests. To examine potential side effects, the animals were subjected to rotarod, Y-maze, and Morris water maze tests to assess motor coordination, memory, and learning. Anticholinesterase activity was determined by direct bioautography and colorimetry based on the Ellman method. The results demonstrated that inhalation of MsEO at doses of 0.1% and 1% significantly reduced anxiety and depressive-like behaviors without impairing memory, learning, or motor coordination in the animals. Moreover, MsEO inhibited acetylcholinesterase with an IC<sub>50</sub> of 0.47 μg/mL. These findings suggest that MsEO has potential therapeutic applications for anxiety and depression disorders, with additional anticholinesterase activity warranting further investigation in cognitive-related conditions. |
| format | Article |
| id | doaj-art-132926beae9146ed982394080abc047e |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-132926beae9146ed982394080abc047e2025-01-24T13:25:13ZengMDPI AGBiomolecules2218-273X2025-01-0115111010.3390/biom15010110Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DCAntônio Quaresma da Silva Júnior0Mariana Maciel Garcia1Wanderson da Silva Farias2Deise Juliane dos Anjos de Sousa3Adenilson de Sousa Barroso4Pablo Luis Baia Figueiredo5Gabriela B. dos Santos6Ricardo Bezerra de Oliveira7Rosa Helena Veras Mourão8Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Rede Bionorte, Universidade Federal do Pará, Belém 66075-110, PA, BrazilLaboratório de Bioprospecção e Biologia Experimental, Universidade Federal do Oeste do Pará, Santarém 68040-255, PA, BrazilLaboratório de Bioprospecção e Biologia Experimental, Universidade Federal do Oeste do Pará, Santarém 68040-255, PA, BrazilLaboratório de Bioprospecção e Biologia Experimental, Universidade Federal do Oeste do Pará, Santarém 68040-255, PA, BrazilLaboratório de Bioprospecção e Biologia Experimental, Universidade Federal do Oeste do Pará, Santarém 68040-255, PA, BrazilPrograma de Pós-Graduação em Biodiversidade e Biotecnologia da Rede Bionorte, Universidade Federal do Pará, Belém 66075-110, PA, BrazilBrazil Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Oeste do Pará, Santarém 68040-255, PA, BrazilPrograma de Pós-Graduação em Biodiversidade e Biotecnologia da Rede Bionorte, Universidade Federal do Pará, Belém 66075-110, PA, BrazilPrograma de Pós-Graduação em Biodiversidade e Biotecnologia da Rede Bionorte, Universidade Federal do Pará, Belém 66075-110, PA, BrazilAromatic plants are rich sources of essential oils (EOs), recognized for their therapeutic properties due to their diversity of phytochemicals. This study investigated the anxiolytic and antidepressant effects of <i>Myrcia sylvatica</i> essential oil (MsEO) through inhalation in an animal model and its in vitro anticholinesterase (AChE) activity. The EO was obtained by hydrodistillation, and its volatile constituents were analyzed by GC-MS. Swiss mice were exposed to doses of 0.1%, 1%, and 2% of the EO via an inhalation apparatus. The anxiolytic activity was assessed using the elevated plus maze and light–dark box tests, while antidepressant activity was evaluated using the tail suspension and forced swimming tests. To examine potential side effects, the animals were subjected to rotarod, Y-maze, and Morris water maze tests to assess motor coordination, memory, and learning. Anticholinesterase activity was determined by direct bioautography and colorimetry based on the Ellman method. The results demonstrated that inhalation of MsEO at doses of 0.1% and 1% significantly reduced anxiety and depressive-like behaviors without impairing memory, learning, or motor coordination in the animals. Moreover, MsEO inhibited acetylcholinesterase with an IC<sub>50</sub> of 0.47 μg/mL. These findings suggest that MsEO has potential therapeutic applications for anxiety and depression disorders, with additional anticholinesterase activity warranting further investigation in cognitive-related conditions.https://www.mdpi.com/2218-273X/15/1/110central nervous systemaromatherapyanxietydepressionAlzheimer’s disease |
| spellingShingle | Antônio Quaresma da Silva Júnior Mariana Maciel Garcia Wanderson da Silva Farias Deise Juliane dos Anjos de Sousa Adenilson de Sousa Barroso Pablo Luis Baia Figueiredo Gabriela B. dos Santos Ricardo Bezerra de Oliveira Rosa Helena Veras Mourão Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DC Biomolecules central nervous system aromatherapy anxiety depression Alzheimer’s disease |
| title | Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DC |
| title_full | Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DC |
| title_fullStr | Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DC |
| title_full_unstemmed | Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DC |
| title_short | Anxiolytic, Antidepressant, and Anticholinesterase Effects of Essential Oil from <i>Myrcia sylvatica</i> (G.Mey.) DC |
| title_sort | anxiolytic antidepressant and anticholinesterase effects of essential oil from i myrcia sylvatica i g mey dc |
| topic | central nervous system aromatherapy anxiety depression Alzheimer’s disease |
| url | https://www.mdpi.com/2218-273X/15/1/110 |
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