Utilization of Date palm (Phoenix dactylifera L.) wastes for bioethanol production using Pichia kudriavzevii strains
Tons of Date palm (Phoenix dactylifera L.) fruit wastes are discarded daily by the date palm processing industries thus leading to serious environmental problems. This study aimed to investigate the potential of date palm fruit wastes as sugary feedstock for bioethanol production using yeasts. Pr...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2022-02-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_217437_2612550ed700c8457bb9b185a645c08d.pdf |
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| Summary: | Tons of Date palm (Phoenix dactylifera L.) fruit wastes are discarded daily by the date
palm processing industries thus leading to serious environmental problems. This study aimed
to investigate the potential of date palm fruit wastes as sugary feedstock for bioethanol
production using yeasts. Proximate analysis of the date fruit wastes revealed a moisture
content of 8.98 %; crude protein (4.39 %), ash (2.35 %), fat (0.2 %), fiber (0.8 %) and
carbohydrate (84.28 %). Sulphuric acid was used for pre-treatment of the date palm fruit
substrate. Fermentation was carried out under shaking and static conditions using Pichia
kudriavzevii yeast strains isolated from date palm fruit wastes. Greater bioethanol yield was
observed when the substrates were fermented under shaking condition. Optimization of the
physical conditions improved the fermentation process faster, and significantly enhanced the
production of bioethanol. An appropriate temperature of 30oC and pH 5 produced high yield of
ethanol (5 %) by Pichia kudriavzevii SGD21, whereas pH 6 for Pichia kudriavzevii SGD30
recorded a higher ethanol yield of 6 %. Under the optimal physical conditions, the
fermentation process resulted in the production of 4 % ethanol after an incubation period of 96
h. Moreover, the Pichia kudriavzevii strains could be recommended for bioethanol production
at 30 % inoculum size, on using sucrose as a carbon source and yeast extract as a nitrogen
source. On using the Fourier-Transform Infrared (FTI) spectroscopy, the detected functional
group of the produced bioethanol was O-H group. Finally, utilization of date palm fruit wastes
and the yeasts strains of Pichia kudriavzevii SGD21 and Pichia kudriavzevii SGD30 can be
exploited for bioethanol production and this could be an effective way for management and
utilization of date palm fruit wastes. |
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| ISSN: | 2537-0286 2537-0294 |