Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
4-Nonylphenols (4-NPs) are persistent endocrine disruptors frequently found in wastewater treatment plant (WWTP) effluents and sewage sludge. This study evaluated the ability of eight bacterial strains that were isolated from sewage sludge to degrade 4-n-NP in an aqueous solution. <i>Bacillus...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/15/12/6408 |
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| Summary: | 4-Nonylphenols (4-NPs) are persistent endocrine disruptors frequently found in wastewater treatment plant (WWTP) effluents and sewage sludge. This study evaluated the ability of eight bacterial strains that were isolated from sewage sludge to degrade 4-n-NP in an aqueous solution. <i>Bacillus safensis</i> CN12, <i>Shewanella putrefaciens</i> CN17, and <i>Alcaligenes faecalis</i> CN8 showed the highest degradation rates, removing 100%, 75%, and 74% of 4-n-NP (10 mg L⁻<sup>1</sup>), with DT<sub>50</sub> values of 0.90, 8.9, and 10.4 days, respectively. Despite the reduction in 4-n-NP concentrations, ecotoxicity assays revealed that the resulting transformation products (TPs) were more toxic than the parent compound. To investigate the potential degradation mechanisms, in silico and gene expression analyses were conducted on <i>B. safensis</i> CN12, revealing a significant upregulation of the multicopper oxidase gene, <i>cotA</i> (7.25-fold), and the ring-cleaving dioxygenase gene, <i>mhqO</i> (13.9-fold). Although the CN12 strain showed potential for mineralization based on gene expression studies, this was not observed in the aqueous solution. However, when 4-n-NP was adsorbed on sludge and treated with CN12 in the presence of hydroxypropyl-β-cyclodextrin (HPBCD) as a bioavailability enhancer, mineralization reached up to 33%, indicating a synergistic effect with the native sludge microbiota. |
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| ISSN: | 2076-3417 |