Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario

Introduction: In HIV-associated tuberculosis cases, identifying nontuberculous Mycobacteria (NTM) from clinical samples has become essential regarding patients’ treatment and prognosis. This study aims to determine the prevalence of different Mycobacteria species from immunocompromised and immun...

Full description

Saved in:
Bibliographic Details
Main Authors: Soumya Dasgupta1, Oindrila Chakraborty2
Format: Article
Language:English
Published: Pasteur Institute of Iran 2022-03-01
Series:Journal of Medical Microbiology and Infectious Diseases
Subjects:
Online Access:https://jommid.pasteur.ac.ir/article-1-415-en.html
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850036427038916608
author Soumya Dasgupta1
Oindrila Chakraborty2
author_facet Soumya Dasgupta1
Oindrila Chakraborty2
author_sort Soumya Dasgupta1
collection DOAJ
description Introduction: In HIV-associated tuberculosis cases, identifying nontuberculous Mycobacteria (NTM) from clinical samples has become essential regarding patients’ treatment and prognosis. This study aims to determine the prevalence of different Mycobacteria species from immunocompromised and immunocompetent patients with suspected tuberculosis attending a tertiary care hospital in Kolkata, India. Methods: Clinical samples from 112 suspected tuberculosis patients were examined by direct microscopy after Ziehl–Neelsen staining. After culture in BacT/ALERT 3D mycobacterial culture system, identification of the causative agents was performed using the GenoType MTCM system. Results: Culture positivity was higher in immunocompromised patients than in immunocompetent in both pulmonary and extrapulmonary samples. In smear-negative samples, culture positivity in immunocompromised patients (50%) was much higher than in immunocompetent patients (26.67%). Of 78 samples, four were NTMs (5.13%), including 1 M. avium, 1 M. abscessus, and 2 M. fortuitum, and 74 were Mycobacterium tuberculosis (MTBC). Two NTMs belonged to immunocompromised patients and two to immunocompetent individuals. The chance of Mycobacterial infection was higher in patients with a lower CD4 count. Both NTMs found in the immunocompromised group were from patients with a CD4 count<100. Conclusion: The present study showed that the MTBC is still the primary causative agent in pulmonary and extrapulmonary tuberculosis in this part of India.
format Article
id doaj-art-13164b4d764f47fc8d319985fa1d3c2a
institution DOAJ
issn 2345-5349
2345-5330
language English
publishDate 2022-03-01
publisher Pasteur Institute of Iran
record_format Article
series Journal of Medical Microbiology and Infectious Diseases
spelling doaj-art-13164b4d764f47fc8d319985fa1d3c2a2025-08-20T02:57:08ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302022-03-01101101310.52547/JoMMID.10.1.10Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian ScenarioSoumya Dasgupta10https://orcid.org/0000-0001-6129-6675Oindrila Chakraborty21https://orcid.org/0000-0002-8089-09681MJN Medical College and Hospital, Coochbehar. West Bengal. India. Zip – 7361012Manipal-Tata Medical College, Jamshedpur. Jharkhand. India. Zip – 831017Introduction: In HIV-associated tuberculosis cases, identifying nontuberculous Mycobacteria (NTM) from clinical samples has become essential regarding patients’ treatment and prognosis. This study aims to determine the prevalence of different Mycobacteria species from immunocompromised and immunocompetent patients with suspected tuberculosis attending a tertiary care hospital in Kolkata, India. Methods: Clinical samples from 112 suspected tuberculosis patients were examined by direct microscopy after Ziehl–Neelsen staining. After culture in BacT/ALERT 3D mycobacterial culture system, identification of the causative agents was performed using the GenoType MTCM system. Results: Culture positivity was higher in immunocompromised patients than in immunocompetent in both pulmonary and extrapulmonary samples. In smear-negative samples, culture positivity in immunocompromised patients (50%) was much higher than in immunocompetent patients (26.67%). Of 78 samples, four were NTMs (5.13%), including 1 M. avium, 1 M. abscessus, and 2 M. fortuitum, and 74 were Mycobacterium tuberculosis (MTBC). Two NTMs belonged to immunocompromised patients and two to immunocompetent individuals. The chance of Mycobacterial infection was higher in patients with a lower CD4 count. Both NTMs found in the immunocompromised group were from patients with a CD4 count<100. Conclusion: The present study showed that the MTBC is still the primary causative agent in pulmonary and extrapulmonary tuberculosis in this part of India.https://jommid.pasteur.ac.ir/article-1-415-en.htmltuberculosisntmimmunocompromisedcd4 count
spellingShingle Soumya Dasgupta1
Oindrila Chakraborty2
Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario
Journal of Medical Microbiology and Infectious Diseases
tuberculosis
ntm
immunocompromised
cd4 count
title Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario
title_full Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario
title_fullStr Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario
title_full_unstemmed Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario
title_short Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario
title_sort mycobacterium tuberculosis dominance over nontuberculous mycobacteria irrespective of immune status an indian scenario
topic tuberculosis
ntm
immunocompromised
cd4 count
url https://jommid.pasteur.ac.ir/article-1-415-en.html
work_keys_str_mv AT soumyadasgupta1 mycobacteriumtuberculosisdominanceovernontuberculousmycobacteriairrespectiveofimmunestatusanindianscenario
AT oindrilachakraborty2 mycobacteriumtuberculosisdominanceovernontuberculousmycobacteriairrespectiveofimmunestatusanindianscenario