A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults

Functional magnetic resonance imaging (fMRI) has been the primary tool in human research for studying and advancing our understanding of neural control of the bladder. Although the current working model identifies several brain regions involved in bladder storage, there are unknowns regarding the fu...

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Main Authors: Arda Bayer, Kris Hoffman, Betsy Salazar, Behnaam Aazhang, Rose Khavari
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Continence
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772973725001547
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author Arda Bayer
Kris Hoffman
Betsy Salazar
Behnaam Aazhang
Rose Khavari
author_facet Arda Bayer
Kris Hoffman
Betsy Salazar
Behnaam Aazhang
Rose Khavari
author_sort Arda Bayer
collection DOAJ
description Functional magnetic resonance imaging (fMRI) has been the primary tool in human research for studying and advancing our understanding of neural control of the bladder. Although the current working model identifies several brain regions involved in bladder storage, there are unknowns regarding the full extent of collaboration among these regions. This study analyzes the changes in the resting state functional connectivity (rsFC) network involving regions of the brain, brainstem, and cerebellum in healthy adults with respect to conventional pairwise rsFC and four rsFC global network statistics. Healthy adult males and females (n=15), ages 22–33, were asked to consume 472–750 mL of water, empty their bladder, and enter the fMRI scanner, after which the “control” resting-state fMRI (rs-fMRI) scans were obtained. Participants remained in the scanner until they indicated a feeling of strong desire to void, or until 10 min had elapsed, at which point “strong desire to void” rs-fMRI scans were acquired. Functional connectivity (FC) analysis was performed using 19 a priori regions of interest (ROIs) in the brain, specifically incorporating the brainstem and cerebellum from previous studies. Statistical significance was gauged via t-tests with Bonferroni correction for multiple hypothesis testing. Our results revealed significance, p<0.05, in the rsFC network, where the average rsFC during the strong sensation state was higher than in the control state. The pontine micturition center and cerebellum were associated with this significant increase in the average degree of 19 ROIs. The contrasts in network statistics were more substantial than the contrasts in pairwise rsFC.
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spelling doaj-art-130f7fdac7a3480abc625b2383a8cfeb2025-08-20T03:30:49ZengElsevierContinence2772-97372025-06-011410189710.1016/j.cont.2025.101897A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adultsArda Bayer0Kris Hoffman1Betsy Salazar2Behnaam Aazhang3Rose Khavari4Department of Electrical and Computer Engineering, Rice University, 6500 Main St., Houston, TX 77030, United States; Corresponding author.Houston Methodist Department of Urology, 6560 Fannin St., Suite 2100, Houston, TX 77030, United States; Houston Methodist Center for Neuroregeneration, 6560 Fannin St., Suite 2100, Houston, TX 77030, United StatesHouston Methodist Department of Urology, 6560 Fannin St., Suite 2100, Houston, TX 77030, United StatesDepartment of Electrical and Computer Engineering, Rice University, 6500 Main St., Houston, TX 77030, United StatesHouston Methodist Department of Urology, 6560 Fannin St., Suite 2100, Houston, TX 77030, United StatesFunctional magnetic resonance imaging (fMRI) has been the primary tool in human research for studying and advancing our understanding of neural control of the bladder. Although the current working model identifies several brain regions involved in bladder storage, there are unknowns regarding the full extent of collaboration among these regions. This study analyzes the changes in the resting state functional connectivity (rsFC) network involving regions of the brain, brainstem, and cerebellum in healthy adults with respect to conventional pairwise rsFC and four rsFC global network statistics. Healthy adult males and females (n=15), ages 22–33, were asked to consume 472–750 mL of water, empty their bladder, and enter the fMRI scanner, after which the “control” resting-state fMRI (rs-fMRI) scans were obtained. Participants remained in the scanner until they indicated a feeling of strong desire to void, or until 10 min had elapsed, at which point “strong desire to void” rs-fMRI scans were acquired. Functional connectivity (FC) analysis was performed using 19 a priori regions of interest (ROIs) in the brain, specifically incorporating the brainstem and cerebellum from previous studies. Statistical significance was gauged via t-tests with Bonferroni correction for multiple hypothesis testing. Our results revealed significance, p<0.05, in the rsFC network, where the average rsFC during the strong sensation state was higher than in the control state. The pontine micturition center and cerebellum were associated with this significant increase in the average degree of 19 ROIs. The contrasts in network statistics were more substantial than the contrasts in pairwise rsFC.http://www.sciencedirect.com/science/article/pii/S2772973725001547Functional connectivity7T fMRIBladder controlUrine storageHealthy adults
spellingShingle Arda Bayer
Kris Hoffman
Betsy Salazar
Behnaam Aazhang
Rose Khavari
A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults
Continence
Functional connectivity
7T fMRI
Bladder control
Urine storage
Healthy adults
title A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults
title_full A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults
title_fullStr A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults
title_full_unstemmed A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults
title_short A deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults
title_sort deeper exploration of functional connectivity of brain regions regulating bladder function in healthy adults
topic Functional connectivity
7T fMRI
Bladder control
Urine storage
Healthy adults
url http://www.sciencedirect.com/science/article/pii/S2772973725001547
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