APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE
Eight protein substances that undergo changes during autolysis were found using the electrophoretic methods with the following mass spectrometric identification. The revealed protein substances have different origin: structural proteins (fragments of troponins T and myosin light chains), and metabol...
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| Format: | Article |
| Language: | English |
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The V.M. Gorbatov All-Russian Meat Research Institute
2018-12-01
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| Series: | Теория и практика переработки мяса |
| Subjects: | |
| Online Access: | https://www.meatjournal.ru/jour/article/view/105 |
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| author | Irina M. Chernukha Anastasiya G. Akhremko |
| author_facet | Irina M. Chernukha Anastasiya G. Akhremko |
| author_sort | Irina M. Chernukha |
| collection | DOAJ |
| description | Eight protein substances that undergo changes during autolysis were found using the electrophoretic methods with the following mass spectrometric identification. The revealed protein substances have different origin: structural proteins (fragments of troponins T and myosin light chains), and metabolic proteins (creatine kinase, pyruvate kinase and alpha-enolase). The decomposition of the fractions of fast skeletal muscle troponin T in 28.0 kDa, 27 kDa and 26.5 kDa was most pronounced.Identification of constitutive proteins and detection of the products of post — mortem degradation of protein molecules make them suitable candidates for meat quality markers and the following study of these specific fragments will lead to better understanding of the proteolytic activities that take part in the post mortem muscle transformation into meat. |
| format | Article |
| id | doaj-art-12fd0d454d0344c694be2109121c6f1e |
| institution | Kabale University |
| issn | 2414-438X 2414-441X |
| language | English |
| publishDate | 2018-12-01 |
| publisher | The V.M. Gorbatov All-Russian Meat Research Institute |
| record_format | Article |
| series | Теория и практика переработки мяса |
| spelling | doaj-art-12fd0d454d0344c694be2109121c6f1e2025-08-20T03:59:57ZengThe V.M. Gorbatov All-Russian Meat Research InstituteТеория и практика переработки мяса2414-438X2414-441X2018-12-0134323710.21323/2414-438X-2018-3-4-32-3784APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUEIrina M. Chernukha0Anastasiya G. Akhremko1V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, MoscowV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, MoscowEight protein substances that undergo changes during autolysis were found using the electrophoretic methods with the following mass spectrometric identification. The revealed protein substances have different origin: structural proteins (fragments of troponins T and myosin light chains), and metabolic proteins (creatine kinase, pyruvate kinase and alpha-enolase). The decomposition of the fractions of fast skeletal muscle troponin T in 28.0 kDa, 27 kDa and 26.5 kDa was most pronounced.Identification of constitutive proteins and detection of the products of post — mortem degradation of protein molecules make them suitable candidates for meat quality markers and the following study of these specific fragments will lead to better understanding of the proteolytic activities that take part in the post mortem muscle transformation into meat.https://www.meatjournal.ru/jour/article/view/105autolysisporkproteomicsmuscle proteintwo-dimensional electrophoresis |
| spellingShingle | Irina M. Chernukha Anastasiya G. Akhremko APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE Теория и практика переработки мяса autolysis pork proteomics muscle protein two-dimensional electrophoresis |
| title | APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE |
| title_full | APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE |
| title_fullStr | APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE |
| title_full_unstemmed | APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE |
| title_short | APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE |
| title_sort | application of proteomic tools the autolytic changes of pork muscular tissue |
| topic | autolysis pork proteomics muscle protein two-dimensional electrophoresis |
| url | https://www.meatjournal.ru/jour/article/view/105 |
| work_keys_str_mv | AT irinamchernukha applicationofproteomictoolstheautolyticchangesofporkmusculartissue AT anastasiyagakhremko applicationofproteomictoolstheautolyticchangesofporkmusculartissue |