Rapid, sensitive, and visual detection of mandarin fish ranavirus and infectious spleen and kidney necrosis virus using an RPA-CRISPR/Cas12a system

Rapid, sensitive, and visual detection of mandarin fish ranavirus and infectious spleen and kidney necrosis virus using an RPA-CRISPR/Cas12a system

Iridoviruses are large cytoplasmic icosahedral viruses that contain dsDNA. Among them, mandarin fish ranavirus (MRV) and infectious spleen and kidney necrosis virus (ISKNV) are particularly notable due to their high contagiousness and pathogenicity. These viruses pose a significant threat to fish aq...

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Bibliographic Details
Main Authors: Zhoutao Lu, Mincong Liang, Chuanrui Li, Yichun Xu, Shaoping Weng, Jianguo He, Changjun Guo
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Microbiology
Subjects:
iridovirus
RPA
CRISPR/Cas12a
ISKNV
ranavirus
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1495777/full
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