A Non-Invasive, Label-Free Method for Examining Tardigrade Anatomy Using Holotomography
Background/Objectives: Holotomography is an advanced imaging technique that enables high-resolution, three-dimensional visualization of microscopic specimens without the need for fixation or staining. Here we aim to apply holotomography technology to image live <i>Hypsibius exemplaris</i>...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Tomography |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2379-139X/11/3/34 |
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| Summary: | Background/Objectives: Holotomography is an advanced imaging technique that enables high-resolution, three-dimensional visualization of microscopic specimens without the need for fixation or staining. Here we aim to apply holotomography technology to image live <i>Hypsibius exemplaris</i> in their native state, avoiding invasive sample preparation procedures and phototoxic effects associated with other imaging modalities. Methods: We use a low concentration of 7% ethanol for tardigrade sedation and sample preparation. Holotomographic images were obtained and reconstructed using the Tomocube HT-X1 system, enabling high-resolution visualization of tardigrade anatomical structures. Results: We captured detailed, label-free holotomography images of both external and internal structures of tardigrade, including the digestive tract, brain, ovary, claws, salivary glands, and musculature. Conclusions: Our findings highlight holotomography as a complementary high-resolution imaging modality that effectively addresses the challenges faced with traditional imaging techniques in tardigrade research. |
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| ISSN: | 2379-1381 2379-139X |