Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice

It was demonstrated previously that mice undergoing an inflammatory reaction induced by subcutaneous (SC) implantation of copper rods, produce humoral factors that initially enhance, but subsequently inhibit, diffusion chamber (DC) granulopoiesis. This provided evidence that granulopoiesis is under...

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Main Authors: Mohamad Hamood, Francis Corazza, Pierre Francois Bluche, Hassan El Teraifi, Pierre Fondu
Format: Article
Language:English
Published: Wiley 1992-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/S0962935192000267
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author Mohamad Hamood
Francis Corazza
Pierre Francois Bluche
Hassan El Teraifi
Pierre Fondu
author_facet Mohamad Hamood
Francis Corazza
Pierre Francois Bluche
Hassan El Teraifi
Pierre Fondu
author_sort Mohamad Hamood
collection DOAJ
description It was demonstrated previously that mice undergoing an inflammatory reaction induced by subcutaneous (SC) implantation of copper rods, produce humoral factors that initially enhance, but subsequently inhibit, diffusion chamber (DC) granulopoiesis. This provided evidence that granulopoiesis is under the control of both humoral stimulators and inhibitors. In order to test the granulopoietic regulatory mechanism in leukaemic mice, we investigated the regulatory role of granulopoietic humoral inhibitors during in vivo granulopoiesis. We noticed that mice suffering from acute myeloid leukaemia (AML) are unable to augment the production of these humoral inhibitory factors when acute inflammation is induced, since no change in DC cell content was observed with or without prior inflammation. Moreover, unlike healthy mice, the serum of leukaemic mice withdrawn during the inhibition phase of acute inflammation did not show any inhibitory activity toward granulocyte—monocyte (GM) colony growth in vitro. Our results also show that increased levels of normal humoral inhibitors do not influence the proliferation and/or differentiation of leukaemic cells implanted in diffusion chamber cultures.
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series Mediators of Inflammation
spelling doaj-art-12f4f15ca1944bf9bff7e7c3b5dca6b62025-08-20T03:55:44ZengWileyMediators of Inflammation0962-93511466-18611992-01-011317117510.1155/S0962935192000267Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic miceMohamad Hamood0Francis Corazza1Pierre Francois Bluche2Hassan El Teraifi3Pierre Fondu4Experimental Hematology Laboratories of Brugmann University Hospital and Joseph Bracops Hospital, Free University of Brussels, Place Van Gehuchten 4, Brussels 1020, BelgiumExperimental Hematology Laboratories of Brugmann University Hospital and Joseph Bracops Hospital, Free University of Brussels, Place Van Gehuchten 4, Brussels 1020, BelgiumExperimental Hematology Laboratories of Brugmann University Hospital and Joseph Bracops Hospital, Free University of Brussels, Place Van Gehuchten 4, Brussels 1020, BelgiumDepartment of Histopathology, Christie Hospital, Manchester, UKExperimental Hematology Laboratories of Brugmann University Hospital and Joseph Bracops Hospital, Free University of Brussels, Place Van Gehuchten 4, Brussels 1020, BelgiumIt was demonstrated previously that mice undergoing an inflammatory reaction induced by subcutaneous (SC) implantation of copper rods, produce humoral factors that initially enhance, but subsequently inhibit, diffusion chamber (DC) granulopoiesis. This provided evidence that granulopoiesis is under the control of both humoral stimulators and inhibitors. In order to test the granulopoietic regulatory mechanism in leukaemic mice, we investigated the regulatory role of granulopoietic humoral inhibitors during in vivo granulopoiesis. We noticed that mice suffering from acute myeloid leukaemia (AML) are unable to augment the production of these humoral inhibitory factors when acute inflammation is induced, since no change in DC cell content was observed with or without prior inflammation. Moreover, unlike healthy mice, the serum of leukaemic mice withdrawn during the inhibition phase of acute inflammation did not show any inhibitory activity toward granulocyte—monocyte (GM) colony growth in vitro. Our results also show that increased levels of normal humoral inhibitors do not influence the proliferation and/or differentiation of leukaemic cells implanted in diffusion chamber cultures.http://dx.doi.org/10.1155/S0962935192000267
spellingShingle Mohamad Hamood
Francis Corazza
Pierre Francois Bluche
Hassan El Teraifi
Pierre Fondu
Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice
Mediators of Inflammation
title Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice
title_full Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice
title_fullStr Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice
title_full_unstemmed Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice
title_short Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice
title_sort granulopoiesis inhibition in acute inflammation comparative studies in healthy and leukaemic mice
url http://dx.doi.org/10.1155/S0962935192000267
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AT pierrefrancoisbluche granulopoiesisinhibitioninacuteinflammationcomparativestudiesinhealthyandleukaemicmice
AT hassanelteraifi granulopoiesisinhibitioninacuteinflammationcomparativestudiesinhealthyandleukaemicmice
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