Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model
Objective. To evaluate the effect of systemic application of cryopreserved human umbilical cord blood mononuclear cells (hUCB-MNCs) in the acute period of spinal cord contusion injury (SCI) on the volume of zone of the spinal cord damage using high-field MRI. Material and Methods. This study was p...
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| Language: | English |
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Ministry of Health of Russian Federation, Federal State Budgetary Institution "Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan"
2024-12-01
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| Series: | Хирургия позвоночника |
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| Online Access: | https://www.spinesurgery.ru/jour/article/view/2194/2171 |
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| author | S.I. Ryabov M.A. Zvyagintseva S.A. Bazanovich Y.V. Morozova S.M. Radaev S.Е. Zuev M.A. Khvostova V.A. Karanadze A.A. Grin V.A. Smirnov |
| author_facet | S.I. Ryabov M.A. Zvyagintseva S.A. Bazanovich Y.V. Morozova S.M. Radaev S.Е. Zuev M.A. Khvostova V.A. Karanadze A.A. Grin V.A. Smirnov |
| author_sort | S.I. Ryabov |
| collection | DOAJ |
| description | Objective. To evaluate the effect of systemic application of cryopreserved human umbilical cord blood mononuclear cells (hUCB-MNCs)
in the acute period of spinal cord contusion injury (SCI) on the volume of zone of the spinal cord damage using high-field MRI.
Material and Methods. This study was performed on adult female Sprague-Dowley rats. Severe contusion SCI was modeled using
the weight-drop method. Cryopreserved hUCB-MNC concentrate, stored in a cryobank for 3–4 years at –196 °C, was administered intravenously 1 day after injury. Locomotor behavior was assessed when animals moved in an open field using the BBB (Basso – Beatty –
Bresnahan) scale for rats. MRI examination of the spinal cord was performed using a Clin Scan 7.0 T device.
Results. At week 6 after injury, a significant increase in the level of restoration of the motor function of the hind limbs (~10 %) was observed in the cell therapy group using hUCB-MNCs relative to the level of the self-healing group (p < 0.05). At the same time, the area
of the posttraumatic cystic cavity decreased significantly (~45 %) and statistically significantly (p < 0.05), as well as its transverse (~38%)
and longitudinal (~41 %) dimensions.
Conclusion. Cryopreserved hUCB-MNCs may be an effective and affordable means of cell therapy for contusion SCI when used in the acute
period of injury. |
| format | Article |
| id | doaj-art-12f3f9ab0e874b6bb189fac0b3c1eb32 |
| institution | DOAJ |
| issn | 1810-8997 2313-1497 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Ministry of Health of Russian Federation, Federal State Budgetary Institution "Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan" |
| record_format | Article |
| series | Хирургия позвоночника |
| spelling | doaj-art-12f3f9ab0e874b6bb189fac0b3c1eb322025-08-20T03:14:23ZengMinistry of Health of Russian Federation, Federal State Budgetary Institution "Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan"Хирургия позвоночника1810-89972313-14972024-12-01214465510.14531/ss2024.4.46-55Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical modelS.I. Ryabov0https://orcid.org/0000-0001-8674-8551M.A. Zvyagintseva1https://orcid.org/0000-0003-3818-7184S.A. Bazanovich2https://orcid.org/0000-0001-5504-5122Y.V. Morozova3https://orcid.org/0000-0002-9575-0749S.M. Radaev4https://orcid.org/0000-0003-4441-3299S.Е. Zuev5https://orcid.org/0000-0003-4860-6848M.A. Khvostova6https://orcid.org/0000-0002-6499-4521V.A. Karanadze7https://orcid.org/0000-0003-0180-9154A.A. Grin8https://orcid.org/0000-0003-3515-8329V.A. Smirnov9https://orcid.org/0000-0003-4096-1087E.I. Chazov National Medical Research Center of Cardiology, Moscow, RussiaE.I. Chazov National Medical Research Center of Cardiology, Moscow, RussiaE.I. Chazov National Medical Research Center of Cardiology, Moscow, RussiaN.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, RussiaN.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, RussiaN.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, RussiaN.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, RussiaN.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, RussiaN.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, RussiaN.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, RussiaObjective. To evaluate the effect of systemic application of cryopreserved human umbilical cord blood mononuclear cells (hUCB-MNCs) in the acute period of spinal cord contusion injury (SCI) on the volume of zone of the spinal cord damage using high-field MRI. Material and Methods. This study was performed on adult female Sprague-Dowley rats. Severe contusion SCI was modeled using the weight-drop method. Cryopreserved hUCB-MNC concentrate, stored in a cryobank for 3–4 years at –196 °C, was administered intravenously 1 day after injury. Locomotor behavior was assessed when animals moved in an open field using the BBB (Basso – Beatty – Bresnahan) scale for rats. MRI examination of the spinal cord was performed using a Clin Scan 7.0 T device. Results. At week 6 after injury, a significant increase in the level of restoration of the motor function of the hind limbs (~10 %) was observed in the cell therapy group using hUCB-MNCs relative to the level of the self-healing group (p < 0.05). At the same time, the area of the posttraumatic cystic cavity decreased significantly (~45 %) and statistically significantly (p < 0.05), as well as its transverse (~38%) and longitudinal (~41 %) dimensions. Conclusion. Cryopreserved hUCB-MNCs may be an effective and affordable means of cell therapy for contusion SCI when used in the acute period of injury.https://www.spinesurgery.ru/jour/article/view/2194/2171spinal cord injurycell therapyhuman umbilical cord blood mononuclear cells |
| spellingShingle | S.I. Ryabov M.A. Zvyagintseva S.A. Bazanovich Y.V. Morozova S.M. Radaev S.Е. Zuev M.A. Khvostova V.A. Karanadze A.A. Grin V.A. Smirnov Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model Хирургия позвоночника spinal cord injury cell therapy human umbilical cord blood mononuclear cells |
| title | Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model |
| title_full | Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model |
| title_fullStr | Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model |
| title_full_unstemmed | Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model |
| title_short | Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model |
| title_sort | cell therapy for spinal cord contusion injury evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model |
| topic | spinal cord injury cell therapy human umbilical cord blood mononuclear cells |
| url | https://www.spinesurgery.ru/jour/article/view/2194/2171 |
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