Cell therapy for spinal cord contusion injury: evaluation of the efficacy of cryopreserved human umbilical cord blood mononuclear cells in a preclinical model
Objective. To evaluate the effect of systemic application of cryopreserved human umbilical cord blood mononuclear cells (hUCB-MNCs) in the acute period of spinal cord contusion injury (SCI) on the volume of zone of the spinal cord damage using high-field MRI. Material and Methods. This study was p...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Ministry of Health of Russian Federation, Federal State Budgetary Institution "Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan"
2024-12-01
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| Series: | Хирургия позвоночника |
| Subjects: | |
| Online Access: | https://www.spinesurgery.ru/jour/article/view/2194/2171 |
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| Summary: | Objective. To evaluate the effect of systemic application of cryopreserved human umbilical cord blood mononuclear cells (hUCB-MNCs)
in the acute period of spinal cord contusion injury (SCI) on the volume of zone of the spinal cord damage using high-field MRI.
Material and Methods. This study was performed on adult female Sprague-Dowley rats. Severe contusion SCI was modeled using
the weight-drop method. Cryopreserved hUCB-MNC concentrate, stored in a cryobank for 3–4 years at –196 °C, was administered intravenously 1 day after injury. Locomotor behavior was assessed when animals moved in an open field using the BBB (Basso – Beatty –
Bresnahan) scale for rats. MRI examination of the spinal cord was performed using a Clin Scan 7.0 T device.
Results. At week 6 after injury, a significant increase in the level of restoration of the motor function of the hind limbs (~10 %) was observed in the cell therapy group using hUCB-MNCs relative to the level of the self-healing group (p < 0.05). At the same time, the area
of the posttraumatic cystic cavity decreased significantly (~45 %) and statistically significantly (p < 0.05), as well as its transverse (~38%)
and longitudinal (~41 %) dimensions.
Conclusion. Cryopreserved hUCB-MNCs may be an effective and affordable means of cell therapy for contusion SCI when used in the acute
period of injury. |
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| ISSN: | 1810-8997 2313-1497 |