Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodies

Abstract Cancer is one of the most dreaded diseases worldwide. Conventional treatments such as surgery, chemotherapy, and radiotherapy have limitations and adverse effects. Cancer immunotherapy and targeted therapies offer new treatment options. Parasite-based cancer therapy shows promise in fightin...

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Main Authors: Maha Mohamed Eissa, Sonia Rifaat Ahmed Allam, Cherine Adel Ismail, Rasha Abdelmawla Ghazala, Nahla El Skhawy, Eman Ibrahim El-said Ibrahim
Format: Article
Language:English
Published: Springer 2025-05-01
Series:Cancer Immunology, Immunotherapy
Subjects:
Online Access:https://doi.org/10.1007/s00262-025-04069-1
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author Maha Mohamed Eissa
Sonia Rifaat Ahmed Allam
Cherine Adel Ismail
Rasha Abdelmawla Ghazala
Nahla El Skhawy
Eman Ibrahim El-said Ibrahim
author_facet Maha Mohamed Eissa
Sonia Rifaat Ahmed Allam
Cherine Adel Ismail
Rasha Abdelmawla Ghazala
Nahla El Skhawy
Eman Ibrahim El-said Ibrahim
author_sort Maha Mohamed Eissa
collection DOAJ
description Abstract Cancer is one of the most dreaded diseases worldwide. Conventional treatments such as surgery, chemotherapy, and radiotherapy have limitations and adverse effects. Cancer immunotherapy and targeted therapies offer new treatment options. Parasite-based cancer therapy shows promise in fighting tumors. Some parasites have anti-cancer properties through multi-mechanistic strategies, with the molecular mimicry theory as a leading explanation for parasites’ anti-cancer effects. This study aimed to explore the existence of shared antigenic proteins between parasites (Trichinella spiralis, Schistosoma mansoni, and Toxoplasma gondii) and cancer cell lines (MCF-7 human breast cancer and A549 human lung cancer). Polyclonal antisera against T. spiralis, S. mansoni, and T. gondii parasites were generated in rabbits. Antibody reactivity with extracts of MCF-7 and A549 cancer cells was detected using SDS-PAGE and immunoblotting. Results documented the molecular mimicry between parasites and cancers as it revealed cross-reactive bands when using T. spiralis antibodies against MCF-7 and A549 cancer cell extracts at approximate molecular weights of 70 and 35 kDa, and with S. mansoni antibodies at an approximate molecular weight of 80 kDa. Toxoplasma gondii antibodies neither reacted with MCF-7 human breast cancer nor A549 human lung cancer cell extracts. Results of this study could establish a foundation for subsequent investigation among a broad range of parasites for molecular mimicry with cancers. Identification, molecular characterization, and investigation of the anti-neoplastic activity of these cross-reactive antigens could shed light on new pathways for the potential development of a novel class of innovative cancer vaccine candidates and therapeutic antibodies of parasitic origin for cancer immunotherapy and targeted therapy.
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spelling doaj-art-12f30634ff4f46fda2796502ae28c7eb2025-08-20T02:06:27ZengSpringerCancer Immunology, Immunotherapy1432-08512025-05-0174711310.1007/s00262-025-04069-1Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodiesMaha Mohamed Eissa0Sonia Rifaat Ahmed Allam1Cherine Adel Ismail2Rasha Abdelmawla Ghazala3Nahla El Skhawy4Eman Ibrahim El-said Ibrahim5Department of Medical Parasitology, Faculty of Medicine, Alexandria UniversityDepartment of Medical Parasitology, Faculty of Medicine, Alexandria UniversityDepartment of Clinical Pharmacology, Faculty of Medicine, Alexandria UniversityDepartment of Medical Biochemistry, Faculty of Medicine, Alexandria UniversityDepartment of Medical Parasitology, Faculty of Medicine, Alexandria UniversityDepartment of Medical Parasitology, Faculty of Medicine, Alexandria UniversityAbstract Cancer is one of the most dreaded diseases worldwide. Conventional treatments such as surgery, chemotherapy, and radiotherapy have limitations and adverse effects. Cancer immunotherapy and targeted therapies offer new treatment options. Parasite-based cancer therapy shows promise in fighting tumors. Some parasites have anti-cancer properties through multi-mechanistic strategies, with the molecular mimicry theory as a leading explanation for parasites’ anti-cancer effects. This study aimed to explore the existence of shared antigenic proteins between parasites (Trichinella spiralis, Schistosoma mansoni, and Toxoplasma gondii) and cancer cell lines (MCF-7 human breast cancer and A549 human lung cancer). Polyclonal antisera against T. spiralis, S. mansoni, and T. gondii parasites were generated in rabbits. Antibody reactivity with extracts of MCF-7 and A549 cancer cells was detected using SDS-PAGE and immunoblotting. Results documented the molecular mimicry between parasites and cancers as it revealed cross-reactive bands when using T. spiralis antibodies against MCF-7 and A549 cancer cell extracts at approximate molecular weights of 70 and 35 kDa, and with S. mansoni antibodies at an approximate molecular weight of 80 kDa. Toxoplasma gondii antibodies neither reacted with MCF-7 human breast cancer nor A549 human lung cancer cell extracts. Results of this study could establish a foundation for subsequent investigation among a broad range of parasites for molecular mimicry with cancers. Identification, molecular characterization, and investigation of the anti-neoplastic activity of these cross-reactive antigens could shed light on new pathways for the potential development of a novel class of innovative cancer vaccine candidates and therapeutic antibodies of parasitic origin for cancer immunotherapy and targeted therapy.https://doi.org/10.1007/s00262-025-04069-1ParasitesCancerMolecular mimicryCancer therapy
spellingShingle Maha Mohamed Eissa
Sonia Rifaat Ahmed Allam
Cherine Adel Ismail
Rasha Abdelmawla Ghazala
Nahla El Skhawy
Eman Ibrahim El-said Ibrahim
Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodies
Cancer Immunology, Immunotherapy
Parasites
Cancer
Molecular mimicry
Cancer therapy
title Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodies
title_full Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodies
title_fullStr Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodies
title_full_unstemmed Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodies
title_short Molecular mimicry between parasites and cancer: a novel approach for developing cancer vaccines and therapeutic antibodies
title_sort molecular mimicry between parasites and cancer a novel approach for developing cancer vaccines and therapeutic antibodies
topic Parasites
Cancer
Molecular mimicry
Cancer therapy
url https://doi.org/10.1007/s00262-025-04069-1
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