Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance
BackgroundPersonalized precision medicine (PPPM) in cancer immunology and oncology is a rapidly advancing field with significant potential. Gliomas, known for their poor prognosis, rank among the most lethal brain tumors. Despite advancements, there remains a critical need for precise, individualize...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1512867/full |
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| author | Meng-Yu Zhao Zhao-Lei Shen Hongzhen Dai Wan-Yan Xu Li-Na Wang Yu- Gu Jie-Hui Zhao Tian-Hang Yu Cun-Zhi Wang Jia-feng Xu Guan-Jun Chen Dong-Hui Chen Wen-Ming Hong Wen-Ming Hong Fang Zhang |
| author_facet | Meng-Yu Zhao Zhao-Lei Shen Hongzhen Dai Wan-Yan Xu Li-Na Wang Yu- Gu Jie-Hui Zhao Tian-Hang Yu Cun-Zhi Wang Jia-feng Xu Guan-Jun Chen Dong-Hui Chen Wen-Ming Hong Wen-Ming Hong Fang Zhang |
| author_sort | Meng-Yu Zhao |
| collection | DOAJ |
| description | BackgroundPersonalized precision medicine (PPPM) in cancer immunology and oncology is a rapidly advancing field with significant potential. Gliomas, known for their poor prognosis, rank among the most lethal brain tumors. Despite advancements, there remains a critical need for precise, individualized treatment strategies.MethodsWe conducted a comprehensive analysis of RNA-seq and microarray data from the TCGA and GEO databases, supplemented by single-cell RNA sequencing (scRNA-seq) data from glioma patients. By integrating single-cell sequencing analysis with foundational experiments, we investigated the molecular variations and cellular interactions within neural glioma cell subpopulations during tumor progression.ResultsOur single-cell sequencing analysis revealed distinct gene expression patterns across glioma cell subpopulations. Notably, differentiation trajectory analysis identified NUSAP1 as a key marker for the terminal subpopulation. We found that elevated NUSAP1 expression correlated with poor prognosis, prompting further investigation of its functional role through both cellular and animal studies.ConclusionsNUSAP1-based risk models hold potential as predictive and therapeutic tools for personalized glioma treatment. In-depth exploration of NUSAP1’s mechanisms in glioblastoma could enhance our understanding of its response to immunotherapy, suggesting that targeting NUSAP1 may offer therapeutic benefits for glioma patients. |
| format | Article |
| id | doaj-art-12eff0e5ade34db98705a3b7719d8ad3 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-12eff0e5ade34db98705a3b7719d8ad32025-02-05T07:32:31ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-02-011610.3389/fimmu.2025.15128671512867Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significanceMeng-Yu Zhao0Zhao-Lei Shen1Hongzhen Dai2Wan-Yan Xu3Li-Na Wang4Yu- Gu5Jie-Hui Zhao6Tian-Hang Yu7Cun-Zhi Wang8Jia-feng Xu9Guan-Jun Chen10Dong-Hui Chen11Wen-Ming Hong12Wen-Ming Hong13Fang Zhang14Department of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaSchool of Nursing, Anhui Medical University, Hefei, ChinaSchool of Nursing, Anhui Medical University, Hefei, ChinaSchool of Nursing, Anhui Medical University, Hefei, ChinaSchool of Nursing, Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaResearch and Experiment Center of Anhui Medical University, Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, Lu’an People’s Hospital, Luan, ChinaDepartment of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaOpen Project of Key Laboratory of Dermatology, Ministry of Education, Anhui Medical University, Hefei, ChinaSchool of Nursing, Anhui Medical University, Hefei, ChinaBackgroundPersonalized precision medicine (PPPM) in cancer immunology and oncology is a rapidly advancing field with significant potential. Gliomas, known for their poor prognosis, rank among the most lethal brain tumors. Despite advancements, there remains a critical need for precise, individualized treatment strategies.MethodsWe conducted a comprehensive analysis of RNA-seq and microarray data from the TCGA and GEO databases, supplemented by single-cell RNA sequencing (scRNA-seq) data from glioma patients. By integrating single-cell sequencing analysis with foundational experiments, we investigated the molecular variations and cellular interactions within neural glioma cell subpopulations during tumor progression.ResultsOur single-cell sequencing analysis revealed distinct gene expression patterns across glioma cell subpopulations. Notably, differentiation trajectory analysis identified NUSAP1 as a key marker for the terminal subpopulation. We found that elevated NUSAP1 expression correlated with poor prognosis, prompting further investigation of its functional role through both cellular and animal studies.ConclusionsNUSAP1-based risk models hold potential as predictive and therapeutic tools for personalized glioma treatment. In-depth exploration of NUSAP1’s mechanisms in glioblastoma could enhance our understanding of its response to immunotherapy, suggesting that targeting NUSAP1 may offer therapeutic benefits for glioma patients.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1512867/fullgliomaNUSAP1cancer immunologysingle-cell sequencingmolecular mechanisms |
| spellingShingle | Meng-Yu Zhao Zhao-Lei Shen Hongzhen Dai Wan-Yan Xu Li-Na Wang Yu- Gu Jie-Hui Zhao Tian-Hang Yu Cun-Zhi Wang Jia-feng Xu Guan-Jun Chen Dong-Hui Chen Wen-Ming Hong Wen-Ming Hong Fang Zhang Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance Frontiers in Immunology glioma NUSAP1 cancer immunology single-cell sequencing molecular mechanisms |
| title | Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance |
| title_full | Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance |
| title_fullStr | Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance |
| title_full_unstemmed | Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance |
| title_short | Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance |
| title_sort | single cell sequencing elucidates the mechanism of nusap1 in glioma and its diagnostic and prognostic significance |
| topic | glioma NUSAP1 cancer immunology single-cell sequencing molecular mechanisms |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1512867/full |
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