Computational limitations and future needs to unravel the full potential of 2’-O-methylation and C/D box snoRNAs

This review evaluates the current state of C/D snoRNA databases and prediction tools in relation to 2’-O-methylation (2'-O-Me). It highlights the limitations of existing resources in accurately annotating and predicting guide snoRNAs, particularly for newly identified 2’-O-Me sites. We emphasiz...

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Bibliographic Details
Main Authors: Christian Ramirez, Elena Perenthaler, Fabio Lauria, Toma Tebaldi, Gabriella Viero
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:RNA Biology
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Online Access:https://www.tandfonline.com/doi/10.1080/15476286.2025.2506712
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Summary:This review evaluates the current state of C/D snoRNA databases and prediction tools in relation to 2’-O-methylation (2'-O-Me). It highlights the limitations of existing resources in accurately annotating and predicting guide snoRNAs, particularly for newly identified 2’-O-Me sites. We emphasize the need for advanced computational approaches specifically tailored to 2’-O-Me to enable the discovery and functional analysis of snoRNAs. Given the growing importance of 2’-O-Me in areas such as cancer epitranscriptomics, ribosome biogenesis, and heterogeneity, existing tools remain inadequate. As 2’-O-Me gains recognition as a potential biomarker and therapeutic target, more sophisticated methods are urgently needed to improve snoRNA annotation and prediction, facilitating biomedical advancements.
ISSN:1547-6286
1555-8584