The reaction mechanism of acetanilide herbicides with urease
The binding of acetochor, pretilachor, butachlor and metolachlor to urease in aqueous solution was studied using UV difference spectrum and fluorescence spectrum methods. According to the UV difference spectrum curve obtained, the UV difference adsorption AA showed rapid change when herbicide concen...
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| Format: | Article |
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Zhejiang University Press
2004-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0210 |
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| author | LIU Hui-jun LIU Wei-ping |
| author_facet | LIU Hui-jun LIU Wei-ping |
| author_sort | LIU Hui-jun |
| collection | DOAJ |
| description | The binding of acetochor, pretilachor, butachlor and metolachlor to urease in aqueous solution was studied using UV difference spectrum and fluorescence spectrum methods. According to the UV difference spectrum curve obtained, the UV difference adsorption AA showed rapid change when herbicide concentrations ranged from 0.0 to 1.6 μmol/L. Red shift occurred in the UV difference spectra of urease and absorbance-peaks were lower. It was shown that the herbicides quenched the urease fluorescence mainly through a static quenching process. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. In case of acetochor, K=1.17×10<sup>3</sup> L/mol and n was 0.81; to pretilachor, K=1.46×10<sup>2</sup> L/mol and n was 0.67; whereas for butachlor, K=29×10<sup>1</sup> L/mol and n was 0.50; and for metolachlor, K=1.49×10<sup>3</sup> L/mol and n was 0.84. |
| format | Article |
| id | doaj-art-12d0872d71ea42d4a918b56c4a2e1ab1 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2004-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-12d0872d71ea42d4a918b56c4a2e1ab12025-08-20T03:15:37ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552004-03-013021021410.3785/1008-9209.2004.02.021010089209The reaction mechanism of acetanilide herbicides with ureaseLIU Hui-junLIU Wei-pingThe binding of acetochor, pretilachor, butachlor and metolachlor to urease in aqueous solution was studied using UV difference spectrum and fluorescence spectrum methods. According to the UV difference spectrum curve obtained, the UV difference adsorption AA showed rapid change when herbicide concentrations ranged from 0.0 to 1.6 μmol/L. Red shift occurred in the UV difference spectra of urease and absorbance-peaks were lower. It was shown that the herbicides quenched the urease fluorescence mainly through a static quenching process. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. In case of acetochor, K=1.17×10<sup>3</sup> L/mol and n was 0.81; to pretilachor, K=1.46×10<sup>2</sup> L/mol and n was 0.67; whereas for butachlor, K=29×10<sup>1</sup> L/mol and n was 0.50; and for metolachlor, K=1.49×10<sup>3</sup> L/mol and n was 0.84.https://www.academax.com/doi/10.3785/1008-9209.2004.02.0210acetanilide herbicidesureaseUV difference spectrumfluorescence spectrum |
| spellingShingle | LIU Hui-jun LIU Wei-ping The reaction mechanism of acetanilide herbicides with urease 浙江大学学报. 农业与生命科学版 acetanilide herbicides urease UV difference spectrum fluorescence spectrum |
| title | The reaction mechanism of acetanilide herbicides with urease |
| title_full | The reaction mechanism of acetanilide herbicides with urease |
| title_fullStr | The reaction mechanism of acetanilide herbicides with urease |
| title_full_unstemmed | The reaction mechanism of acetanilide herbicides with urease |
| title_short | The reaction mechanism of acetanilide herbicides with urease |
| title_sort | reaction mechanism of acetanilide herbicides with urease |
| topic | acetanilide herbicides urease UV difference spectrum fluorescence spectrum |
| url | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0210 |
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