Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV
Abstract Background Pseudorabies (PR) is a highly contagious disease, and it causes significant economic losses to the global swine industry. Vaccination plays an important role in the prevention and control of pseudorabies virus (PRV). To evaluate vaccine efficacy, there is a need for a quick and s...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-025-04913-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849764173924270080 |
|---|---|
| author | Suzhen Yang Yaning Sun Yunrui Xing Lu Fan Yongliang Li Yanli Shang Shujun Chai Yunchao Liu |
| author_facet | Suzhen Yang Yaning Sun Yunrui Xing Lu Fan Yongliang Li Yanli Shang Shujun Chai Yunchao Liu |
| author_sort | Suzhen Yang |
| collection | DOAJ |
| description | Abstract Background Pseudorabies (PR) is a highly contagious disease, and it causes significant economic losses to the global swine industry. Vaccination plays an important role in the prevention and control of pseudorabies virus (PRV). To evaluate vaccine efficacy, there is a need for a quick and straight forward method to monitor PRV-induced antibody levels in practice. Results A time-resolved fluorescence immunochromatographic (TRFIC) strip was developed for the serological detection of PRV gB antibodies in swine. Following systematic analysis and evaluation, the assay demonstrated a high degree of correlation with established reference method. The positive and negative coincidence rates between the TRFIC strip and ELISA were 96.8% and 94.2%, respectively. Furthermore, comprehensive analytical and comparative assessments revealed that the TRFIC strip exhibited no cross-reactivity with antibodies against other porcine pathogens. Conclusion Given its high specificity, sensitivity, and convenience, the TRFIC strip is suitable for on-site detection of PRV gB antibodies and can serve as a valuable tool for monitoring PRV immune status in animal populations. |
| format | Article |
| id | doaj-art-12cd2062f2054b1981df130bef006b92 |
| institution | DOAJ |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-12cd2062f2054b1981df130bef006b922025-08-20T03:05:13ZengBMCBMC Veterinary Research1746-61482025-07-012111910.1186/s12917-025-04913-7Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRVSuzhen Yang0Yaning Sun1Yunrui Xing2Lu Fan3Yongliang Li4Yanli Shang5Shujun Chai6Yunchao Liu7Institute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesAnhui Divinity Biological Products Co., LtdInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesAbstract Background Pseudorabies (PR) is a highly contagious disease, and it causes significant economic losses to the global swine industry. Vaccination plays an important role in the prevention and control of pseudorabies virus (PRV). To evaluate vaccine efficacy, there is a need for a quick and straight forward method to monitor PRV-induced antibody levels in practice. Results A time-resolved fluorescence immunochromatographic (TRFIC) strip was developed for the serological detection of PRV gB antibodies in swine. Following systematic analysis and evaluation, the assay demonstrated a high degree of correlation with established reference method. The positive and negative coincidence rates between the TRFIC strip and ELISA were 96.8% and 94.2%, respectively. Furthermore, comprehensive analytical and comparative assessments revealed that the TRFIC strip exhibited no cross-reactivity with antibodies against other porcine pathogens. Conclusion Given its high specificity, sensitivity, and convenience, the TRFIC strip is suitable for on-site detection of PRV gB antibodies and can serve as a valuable tool for monitoring PRV immune status in animal populations.https://doi.org/10.1186/s12917-025-04913-7PRVgBTime-resolved fluorescence immunochromatographic stripELISA |
| spellingShingle | Suzhen Yang Yaning Sun Yunrui Xing Lu Fan Yongliang Li Yanli Shang Shujun Chai Yunchao Liu Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV BMC Veterinary Research PRV gB Time-resolved fluorescence immunochromatographic strip ELISA |
| title | Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV |
| title_full | Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV |
| title_fullStr | Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV |
| title_full_unstemmed | Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV |
| title_short | Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV |
| title_sort | development of a time resolved fluorescence immunochromatographic strip for gb antibody detection of prv |
| topic | PRV gB Time-resolved fluorescence immunochromatographic strip ELISA |
| url | https://doi.org/10.1186/s12917-025-04913-7 |
| work_keys_str_mv | AT suzhenyang developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv AT yaningsun developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv AT yunruixing developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv AT lufan developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv AT yongliangli developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv AT yanlishang developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv AT shujunchai developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv AT yunchaoliu developmentofatimeresolvedfluorescenceimmunochromatographicstripforgbantibodydetectionofprv |