Immobilization of Peroxidase onto Magnetite Modified Polyaniline
The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/716374 |
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| _version_ | 1849308912256286720 |
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| author | Eduardo Fernandes Barbosa Fernando Javier Molina Flavio Marques Lopes Pedro Antonio García-Ruíz Samantha Salomão Caramori Kátia Flávia Fernandes |
| author_facet | Eduardo Fernandes Barbosa Fernando Javier Molina Flavio Marques Lopes Pedro Antonio García-Ruíz Samantha Salomão Caramori Kátia Flávia Fernandes |
| author_sort | Eduardo Fernandes Barbosa |
| collection | DOAJ |
| description | The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein). The optimum pH for immobilization was displaced by the effect of the partition of protons produced in the microenvironment by the magnetite. The tests of repeated use have shown that PANImG-HRP can be used for 13 cycles with maintenance of 50% of the initial activity. |
| format | Article |
| id | doaj-art-12b4f121705b40bc8be4010d29f8f451 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-12b4f121705b40bc8be4010d29f8f4512025-08-20T03:54:20ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/716374716374Immobilization of Peroxidase onto Magnetite Modified PolyanilineEduardo Fernandes Barbosa0Fernando Javier Molina1Flavio Marques Lopes2Pedro Antonio García-Ruíz3Samantha Salomão Caramori4Kátia Flávia Fernandes5Laboratório de Química de Proteínas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Codigo Postal 131, 74001-970 Goiânia, GO, BrazilGrupo de Química de Carboydratos y Biotecnología de Alimentos, Departamento de Química Orgánica, Universidad de Murcia, Campus Espinardo, 34100 Murcia, SpainLaboratório de Química de Proteínas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Codigo Postal 131, 74001-970 Goiânia, GO, BrazilGrupo de Química de Carboydratos y Biotecnología de Alimentos, Departamento de Química Orgánica, Universidad de Murcia, Campus Espinardo, 34100 Murcia, SpainUnidade Universitária de Ciências Exatas e Tecnológicas, Campus Dr. Henrique Santillo, Universidade Estadual de Goiás, Rodovia BR 153 Km 98, Codigo Postal 459, 75132-903 Anápolis, GO, BrazilLaboratório de Química de Proteínas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Codigo Postal 131, 74001-970 Goiânia, GO, BrazilThe present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein). The optimum pH for immobilization was displaced by the effect of the partition of protons produced in the microenvironment by the magnetite. The tests of repeated use have shown that PANImG-HRP can be used for 13 cycles with maintenance of 50% of the initial activity.http://dx.doi.org/10.1100/2012/716374 |
| spellingShingle | Eduardo Fernandes Barbosa Fernando Javier Molina Flavio Marques Lopes Pedro Antonio García-Ruíz Samantha Salomão Caramori Kátia Flávia Fernandes Immobilization of Peroxidase onto Magnetite Modified Polyaniline The Scientific World Journal |
| title | Immobilization of Peroxidase onto Magnetite Modified Polyaniline |
| title_full | Immobilization of Peroxidase onto Magnetite Modified Polyaniline |
| title_fullStr | Immobilization of Peroxidase onto Magnetite Modified Polyaniline |
| title_full_unstemmed | Immobilization of Peroxidase onto Magnetite Modified Polyaniline |
| title_short | Immobilization of Peroxidase onto Magnetite Modified Polyaniline |
| title_sort | immobilization of peroxidase onto magnetite modified polyaniline |
| url | http://dx.doi.org/10.1100/2012/716374 |
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