MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1
Let-7 miRNA family has been proved as a key regulator of mesenchymal stem cells’ (MSCs’) biological features. However, whether let-7b could affect the differentiation or proliferation of periodontal ligament stem cells (PDLSCs) is still unknown. Here, we found that the expression of hsa-let-7b was v...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/5791181 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850235610983301120 |
|---|---|
| author | Lin Fu Na Li Yu Ye Xiaying Ye Tong Xiao Xiao Wu Yanxia Ma Jinhua Yu |
| author_facet | Lin Fu Na Li Yu Ye Xiaying Ye Tong Xiao Xiao Wu Yanxia Ma Jinhua Yu |
| author_sort | Lin Fu |
| collection | DOAJ |
| description | Let-7 miRNA family has been proved as a key regulator of mesenchymal stem cells’ (MSCs’) biological features. However, whether let-7b could affect the differentiation or proliferation of periodontal ligament stem cells (PDLSCs) is still unknown. Here, we found that the expression of hsa-let-7b was visibly downregulated after mineralization induction of PDLSCs. After transfected with hsa-let-7b mimics or inhibitor reagent, the proliferation ability of PDLSCs was detected by cell counting kit-8 (CCK-8), flow cytometry, and 5-ethynyl-2-deoxyuridine (EdU) assay. On the other hand, the osteogenic differentiation capacity was detected by alkaline phosphatase (ALP) staining and activity, alizarin red staining, Western blot, and quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). We verified that hsa-let-7b did not significantly impact the proliferation ability of PDLSCs, but it could curb the osteogenic differentiation of PDLSCs. Besides, we predicted CTHRC1 acts as the downstream gene of hsa-let-7b to affect this process. Moreover, the combination of CTHRC1 and hsa-let-7b was verified by dual luciferase reporter assay. Our results demonstrated that the osteogenic differentiation of PDLSCs was enhanced after inhibiting hsa-let-7b, while was weakened after cotransfection with Si-CTHRC1. Collectively, hsa-let-7b can repress the osteogenic differentiation of PDLSCs by regulating CTHRC1. |
| format | Article |
| id | doaj-art-12b4ea64bdb24242a3bef7e4527391e5 |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-12b4ea64bdb24242a3bef7e4527391e52025-08-20T02:02:12ZengWileyStem Cells International1687-96782021-01-01202110.1155/2021/5791181MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1Lin Fu0Na Li1Yu Ye2Xiaying Ye3Tong Xiao4Xiao Wu5Yanxia Ma6Jinhua Yu7Key Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityKey Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityKey Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityKey Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityKey Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityKey Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityKey Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityKey Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical UniversityLet-7 miRNA family has been proved as a key regulator of mesenchymal stem cells’ (MSCs’) biological features. However, whether let-7b could affect the differentiation or proliferation of periodontal ligament stem cells (PDLSCs) is still unknown. Here, we found that the expression of hsa-let-7b was visibly downregulated after mineralization induction of PDLSCs. After transfected with hsa-let-7b mimics or inhibitor reagent, the proliferation ability of PDLSCs was detected by cell counting kit-8 (CCK-8), flow cytometry, and 5-ethynyl-2-deoxyuridine (EdU) assay. On the other hand, the osteogenic differentiation capacity was detected by alkaline phosphatase (ALP) staining and activity, alizarin red staining, Western blot, and quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). We verified that hsa-let-7b did not significantly impact the proliferation ability of PDLSCs, but it could curb the osteogenic differentiation of PDLSCs. Besides, we predicted CTHRC1 acts as the downstream gene of hsa-let-7b to affect this process. Moreover, the combination of CTHRC1 and hsa-let-7b was verified by dual luciferase reporter assay. Our results demonstrated that the osteogenic differentiation of PDLSCs was enhanced after inhibiting hsa-let-7b, while was weakened after cotransfection with Si-CTHRC1. Collectively, hsa-let-7b can repress the osteogenic differentiation of PDLSCs by regulating CTHRC1.http://dx.doi.org/10.1155/2021/5791181 |
| spellingShingle | Lin Fu Na Li Yu Ye Xiaying Ye Tong Xiao Xiao Wu Yanxia Ma Jinhua Yu MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1 Stem Cells International |
| title | MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1 |
| title_full | MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1 |
| title_fullStr | MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1 |
| title_full_unstemmed | MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1 |
| title_short | MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1 |
| title_sort | microrna hsa let 7b regulates the osteogenic differentiation of human periodontal ligament stem cells by targeting cthrc1 |
| url | http://dx.doi.org/10.1155/2021/5791181 |
| work_keys_str_mv | AT linfu micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 AT nali micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 AT yuye micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 AT xiayingye micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 AT tongxiao micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 AT xiaowu micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 AT yanxiama micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 AT jinhuayu micrornahsalet7bregulatestheosteogenicdifferentiationofhumanperiodontalligamentstemcellsbytargetingcthrc1 |