Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cells
In the modern world, such a situation has developed that the development of nuclear energy and the use of sources of ionizing radiation in various fields of activity have created a potential threat of radiation hazard to humans. In this connection, the actual direction is the study of ways to increa...
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Saint-Petersburg Research Institute of Radiation Hygiene after Professor P.V. Ramzaev
2023-04-01
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| Series: | Радиационная гигиена |
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| Online Access: | https://www.radhyg.ru/jour/article/view/932 |
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| author | P. S. Kuptsova L. N. Komarova E. R. Vypova |
| author_facet | P. S. Kuptsova L. N. Komarova E. R. Vypova |
| author_sort | P. S. Kuptsova |
| collection | DOAJ |
| description | In the modern world, such a situation has developed that the development of nuclear energy and the use of sources of ionizing radiation in various fields of activity have created a potential threat of radiation hazard to humans. In this connection, the actual direction is the study of ways to increase the radioresistance of human cells and tissues to the action of ionizing radiation. In addition, radioprotective compounds are also important in radiotherapy, since normal tissues of patients must be protected from radiation damage when using high doses of radiation in the treatment of malignant neoplasms. However, the radioprotectors currently used have some disadvantages. The aim of this work is to study the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of gamma radiation and radiation of 12C ions on tumor (SK-N-BE neuroblastoma) and normal (hTERT fibroblasts) human cells. The effect of radiation was evaluated according to the criteria of cell survival in culture, doubling time and clonogenic activity. It has been shown that the use of fumaric acid and 3-hydroxypyridine fumarate has a radioprotective effect on normal and tumor cells when they are irradiated with gamma radiation at doses of 1, 4, 6 and 10 Gy. The use of fumaric acid and 3-hydroxypyridine fumarate does not have a radioprotective effect on tumor cells when they are irradiated with 12C ions. A comparative analysis of the results of the action of the preparations with gamma rays and 12C ions showed a significant dependence of the manifestation of modifying properties on the quality of radiation. The study of cell doubling time showed that the presence of drugs in the control did not increase this indicator. On the contrary, under the action of gamma radiation at a dose of 10 Gy, the preparations reduced the doubling time of fibroblasts by more than two times and the doubling time of neuroblastoma cells by almost 1.5 times. The data obtained indicate that the antioxidant properties of the studied preparations open up new possibilities for modifying the action of ionizing radiation in the treatment of oncological diseases. The use of fumaric acid and 3-hydroxypyridine fumarate will reduce the radiation load on healthy cells, including reducing the effect of secondary products on healthy cells beyond the Bragg peak under the action of carbon ion therapy. |
| format | Article |
| id | doaj-art-12a495e7874b4b2babbfec9e9cfa8deb |
| institution | Kabale University |
| issn | 1998-426X |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Saint-Petersburg Research Institute of Radiation Hygiene after Professor P.V. Ramzaev |
| record_format | Article |
| series | Радиационная гигиена |
| spelling | doaj-art-12a495e7874b4b2babbfec9e9cfa8deb2025-08-20T03:43:50ZengSaint-Petersburg Research Institute of Radiation Hygiene after Professor P.V. RamzaevРадиационная гигиена1998-426X2023-04-01161323910.21514/1998-426X-2023-16-1-32-39784Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cellsP. S. Kuptsova0L. N. Komarova1E. R. Vypova2Obninsk Institute for Nuclear Power Engineering; A. Tsyb National Medical Research Radiological Centre of the Ministry of Health of the Russian FederationObninsk Institute for Nuclear Power EngineeringObninsk Institute for Nuclear Power EngineeringIn the modern world, such a situation has developed that the development of nuclear energy and the use of sources of ionizing radiation in various fields of activity have created a potential threat of radiation hazard to humans. In this connection, the actual direction is the study of ways to increase the radioresistance of human cells and tissues to the action of ionizing radiation. In addition, radioprotective compounds are also important in radiotherapy, since normal tissues of patients must be protected from radiation damage when using high doses of radiation in the treatment of malignant neoplasms. However, the radioprotectors currently used have some disadvantages. The aim of this work is to study the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of gamma radiation and radiation of 12C ions on tumor (SK-N-BE neuroblastoma) and normal (hTERT fibroblasts) human cells. The effect of radiation was evaluated according to the criteria of cell survival in culture, doubling time and clonogenic activity. It has been shown that the use of fumaric acid and 3-hydroxypyridine fumarate has a radioprotective effect on normal and tumor cells when they are irradiated with gamma radiation at doses of 1, 4, 6 and 10 Gy. The use of fumaric acid and 3-hydroxypyridine fumarate does not have a radioprotective effect on tumor cells when they are irradiated with 12C ions. A comparative analysis of the results of the action of the preparations with gamma rays and 12C ions showed a significant dependence of the manifestation of modifying properties on the quality of radiation. The study of cell doubling time showed that the presence of drugs in the control did not increase this indicator. On the contrary, under the action of gamma radiation at a dose of 10 Gy, the preparations reduced the doubling time of fibroblasts by more than two times and the doubling time of neuroblastoma cells by almost 1.5 times. The data obtained indicate that the antioxidant properties of the studied preparations open up new possibilities for modifying the action of ionizing radiation in the treatment of oncological diseases. The use of fumaric acid and 3-hydroxypyridine fumarate will reduce the radiation load on healthy cells, including reducing the effect of secondary products on healthy cells beyond the Bragg peak under the action of carbon ion therapy.https://www.radhyg.ru/jour/article/view/932radioprotectorsgamma radiation12с ionsfibroblastsneuroblastomasurvivalclonogenic analysisdoubling timefumaric acid3-oxypyridine fumarate |
| spellingShingle | P. S. Kuptsova L. N. Komarova E. R. Vypova Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cells Радиационная гигиена radioprotectors gamma radiation 12с ions fibroblasts neuroblastoma survival clonogenic analysis doubling time fumaric acid 3-oxypyridine fumarate |
| title | Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cells |
| title_full | Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cells |
| title_fullStr | Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cells |
| title_full_unstemmed | Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cells |
| title_short | Study of the radioprotective properties of fumaric acid and 3-hydroxypyridine fumarate under the action of ionizing radiation on human cells |
| title_sort | study of the radioprotective properties of fumaric acid and 3 hydroxypyridine fumarate under the action of ionizing radiation on human cells |
| topic | radioprotectors gamma radiation 12с ions fibroblasts neuroblastoma survival clonogenic analysis doubling time fumaric acid 3-oxypyridine fumarate |
| url | https://www.radhyg.ru/jour/article/view/932 |
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